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themes/01-organoid-equipment.md
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@@ -61,6 +61,7 @@ The state diagram shows the canonical CelVivo → 3Brain workflow: 3D suspension
- **Bosnjakovic 2025 bioRxiv (PAH HepG2 3D spheroid, ClinoStar dynamic culture)** — 21-day maturation + 24h/96h BaP/BBF exposure; **4 metabolic markers (phenylalanine, TCA intermediates, GSH depletion, sphingolipid dysregulation) only appear in 3D, completely invisible in 2D**. Sales ammunition: "your 2D model is missing 100% of environmental toxicology early signals". Synthesis at [`/sources/literature-cel-bosnjakovic-2025-pah-deepread.md`](/sources/literature-cel-bosnjakovic-2025-pah-deepread.md).
- **Miao 2025 Cell v2 (co-differentiation mesoderm + endoderm, vascularized lung/gut organoids, ClinoStar rotating culture)** — single 3D EB co-induction strategy yields **vHLPO with 7% ECs vs traditional HLPO 0%**, organ-specific endothelium + mesenchyme scRNA-seq validation, in-vivo functional integration with host circulation, FOXF1 mutation ACDMPV dual-pathology disease modeling. Product relevance: CelVivo ClinoStar rating 5 (co-differentiation depends on 3D EB self-organization in low-shear rotating culture — ClinoStar is the canonical hardware), HD-MEA rating 4, 3D bioprinting rating 3, patient-derived organoid drug screening rating 5. Synthesis at [`/sources/literature-miao-2025-cell-vascularization-v2-deepread.md`](/sources/literature-miao-2025-cell-vascularization-v2-deepread.md).
- **Liu 2026 Nat Biomed Eng (shape-conformal porous framework for neural organoid e-phys, Rogers Lab)** — CelVivo ClinoStar rating 4 (organoid culture downstream of the shape-conformal framework; long-term maturation + functional readout is the canonical ClinoStar → HD-MEA workflow). Synthesis at [`/sources/literature-liu-2026-nbme-shape-conformal-organoid-deepread.md`](/sources/literature-liu-2026-nbme-shape-conformal-organoid-deepread.md). See 3Brain evidence for the HD-MEA read side.
- **Liang 2026 Sci Rep (mouse liver organoid 3-轨道 + fibrosis modeling, 2026-08-12)** — CelVivo ClinoStar rating 4 (P1-P10 长程扩增对应旋转培养 30+ 天无剪切; single DAPT only sustains P5, DAPT+Dex 协同才实现 P10 稳定成熟), CelVivo ClinoReactor rating 4 (单组织 3 轨道 [Hep/Cho/HSC] 分通道灌流; TGFβ 时间程序精确切换实现纤维化激活模型), CelVivo PhaseHolographic 成像 rating 3 (3D 球体直径/CV 实时 QC), 3Brain HD-MEA rating 3 (肝-神经互作延伸场景). 3 轨道 from 单份小鼠肝组织 (密度梯度离心 + 差速贴壁) → DAPT+Dex P1-P10 长程稳定成熟 (ALB / PAS / LDL / CYP 跨代保持) → TGFβ 5-7 天激活 qHSC → aHSC (Acta2/Col1a1 上调) → aHSC 共培养诱导 Hep-Orgs EMT (Snail/Twist/Vimentin ↑; ALB/CK8 ↓; Lgr5/EpCAM ↓; Ki67 ↓) = 体外肝纤维化模型. Wiki.js Id=1716, NocoDB Id=770, PMID 41857098. Page 946 9 项 SOP 重写版 + page 934 修齐 标杆 (4-commit LDR cycle 2026-08-12). **First deep-read whose `related:` field already names `[[MOC-类器官与3D培养]]`** on the day of publication — confirms MOC pattern in active use on real deep-reads. Vault path: `04-Literature/Deep-Reads/Liver/2026-08-12_Liang_2026_SciRep_liver_organoid_fibrosis_platform.md` (170 lines). Sales ammunition: "你的肝类器官只能短期培养? DAPT+Dex P1-P10 稳定成熟 + 单组织 3 轨道 + 14 天纤维化模型 — ClinoStar + ClinoReactor 一站搞定". Customer entry: 药企临床前 (抗纤维化药筛 14+ 天 3D 多细胞模型) + 临床肝活检中心 (1-2 mg 组织 → 3 周药敏 → ClinoReactor 6 通道一次测 6 药). Tracks in `/commitments.md` as `liang-2026-celvivo-clinostar-positioning`.
- **Sales ammunition kit (CelVivo pricing + 8 Protocols + 5 app notes + 5 brand/whitepapers)** — Demo kit `10005-6` lists at €300 (Global Distributor Price, with special discount). 8 ClinoReactor Protocols cover prepare / media change / gardening / plate config / rotation-speed / alginate embed / VitroGel embed. 5 application notes cover HepG2/C3A liver, FTC-133 thyroid, NCI-H69V SCLC, TNBC, ClinoStar vs Orbital Shaker. Synthesis at [`/sources/sales-ammunition.md`](/sources/sales-ammunition.md).
- **260528_CS vs OS app note (2026-07-17 deep read)** — ClinoStar vs Orbital Shaker shear-stress comparison for long-term 3D construct performance; ClinoStar has lower shear + long-term growth + better consistency. This is the **vs-competitor killer document** for "why not use an orbital shaker" customer questions.
- **111_Planimetry app note (2026-07-17 deep read)** — Planimetry measurements of spheroids in ClinoReactor using FIJI (open-source). Standard answer for "how to quantify spheroids".
@@ -110,6 +111,7 @@ The state diagram shows the canonical CelVivo → 3Brain workflow: 3D suspension
| What is Miao 2025's vascularized lung/gut organoid story? | [`/sources/literature-miao-2025-cell-vascularization-v2-deepread.md`](/sources/literature-miao-2025-cell-vascularization-v2-deepread.md) |
| What is Liu 2026's shape-conformal neural organoid e-phys story? | [`/sources/literature-liu-2026-nbme-shape-conformal-organoid-deepread.md`](/sources/literature-liu-2026-nbme-shape-conformal-organoid-deepread.md) |
| What is Sharma 2026's vascularized retinal organoid RGC story? | [`/sources/literature-sharma-2026-cell-stem-cell-vascularized-retinal-organoid-rgc-deepread.md`](/sources/literature-sharma-2026-cell-stem-cell-vascularized-retinal-organoid-rgc-deepread.md) |
| What is Liang 2026's liver organoid fibrosis platform story? | `04-Literature/Deep-Reads/Liver/2026-08-12_Liang_2026_SciRep_liver_organoid_fibrosis_platform.md` (vault path; deep-read synthesis not yet split out to `/sources/`) |
| What distributors am I actually working with? | [`/sources/agents.md`](/sources/agents.md) |
| Which Chinese dealers carry our brand? | [`/sources/distributors-china-2026.md`](/sources/distributors-china-2026.md) |
| What regulatory shifts affect our Chinese pharma deals? | [`/commitments.md`](/commitments.md) China CDE / Order No. 818 / China CGT items |
themes/05-robin-research.md
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@@ -22,7 +22,7 @@ This is the synthesis page for **Robin 个人研究项目** — the durable body
```mermaid
erDiagram
VAULT["Obsidian vault (d97b39ed)"] ||--o{ DEEPREADS["Deep-read notes ~1,202"]
VAULT["Obsidian vault (297f106d)"] ||--o{ DEEPREADS["Deep-read notes ~1,204"]
VAULT ||--o{ TRAIN["Sales training (CelVivo赋能培训 v1-v9)"]
VAULT ||--o{ SALES["Active sales pipeline (惠百生物 PO + 中间商)"]
VAULT ||--o{ CRM["Distributor profiles (董慧群 + 待补)"]
@@ -49,18 +49,18 @@ The diagram shows Robin's research substrate: a single Obsidian vault with 8 buc
## Knowledge substrate: Obsidian vault
Snapshot from [`/sources/git-repo.md`](/sources/git-repo.md) (last fetch 2026-08-10T18:06:19Z):
Snapshot from [`/sources/git-repo.md`](/sources/git-repo.md) (last fetch 2026-08-12T18:08:49Z):
| Field | Value |
| --- | --- |
| Repo path | `/home/ldw/workspace` |
| Branch | `main` |
| HEAD | `d97b39edb328b7e15d28de4a54a6d0bc2dd51274` (2026-08-10) |
| Ahead of origin/main | 12 commits new since 2026-08-03 fetch (Fernandez Vallone 2026 + Microbioreactor 2026 + Liu 2026 v2-v11 + 4 经销商 hook 升级 + Wang 2024 Theranostics + **Phase A metadata batch + 2 sample runs**); plus 19 untracked files in working tree |
| HEAD | `297f106d2b137af3480261052ff080cbc48d5b48` (2026-08-12) |
| Ahead of origin/main | 13 commits new since 2026-08-03 fetch (Fernandez Vallone 2026 + Microbioreactor 2026 + Liu 2026 v2-v11 + 4 经销商 hook 升级 + Wang 2024 Theranostics + **Phase A metadata batch + 2 sample runs** + **Liang 2026 liver organoid fibrosis platform 4-commit LDR cycle**); plus 19 untracked files in working tree |
| Auth | local path only — no remote, no credentials |
| Working-tree state | dirty (1 nested-submodule + 1 untracked dir + 19 untracked files including 8 new `02-Clients/Agents/区域-2026-0807.md`) |
| Raw manifest | `~/.openwiki/connectors/git-repo/raw/2026-08-10T18-06-18-966Z/manifest.json` |
| **Latest cluster (2026-08-10)** | **Phase A metadata batch** (`d97b39ed`) + **MOC 方案 1 样品** (`6b717aef`, first MOC `MOC-类器官与3D培养.md`) + **wang2024 方案 2 样品** (`3b929c2f`, 7 image alt descriptions) — frontmatter-hygiene + MOC-discipline sprint, no new external evidence |
| Raw manifest | `~/.openwiki/connectors/git-repo/raw/2026-08-12T18-08-49-138Z/manifest.json` |
| **Latest cluster (2026-08-12)** | **Liang 2026 Sci Rep liver organoid fibrosis platform 4-commit LDR cycle** (`e542c977` adds file 186 lines → `8699a154` page 946 9 项 SOP 重写版 105+/122- → `aacba7aa` frontmatter title 改中文 1 line → `297f106d` page 934 修齐 5+/4-) — **net-new external evidence** (DOI 10.1038/s41598-026-42990-2, PMID 41857098, mouse liver 3-轨道 Hep-Orgs + Cho-Orgs + HSC + DAPT+Dex P1-P10 长程成熟 + TGFβ 激活体外纤维化模型) |
### Vault navigation index (top-level folders)
@@ -98,7 +98,7 @@ Snapshot from [`/sources/git-repo.md`](/sources/git-repo.md) (last fetch 2026-08
| `Brain/` | 11 | brain organoid focus |
| `Brain-Tumor/` | 9 | brain tumor organoid |
| `Cancer/` | 6 | cancer organoid |
| `Liver/` | 6 | liver organoid / HepG2 |
| `Liver/` | 7 | liver organoid / HepG2 (Liang 2026 4-commit LDR cycle 2026-08-12) |
| `Other/` | 6 | uncategorised |
| `RegenMed/` | 1 | Ronawk Bio-Block MSC 4-week (added 2026-07-28) |
| `3Brain MEA/` | 3 | 3Brain HD-MEA papers |
@@ -106,7 +106,7 @@ Snapshot from [`/sources/git-repo.md`](/sources/git-repo.md) (last fetch 2026-08
| `Kidney/` | 2 | kidney organoid |
| `Retinal/` | 1 | retinal organoid |
Total ≈ 1,202 deep-read notes. The 4 vendor subfolders (Celvivo / 3Brain / Cellink / Organoid) cover the canonical product KB per the wiki goal focus areas.
Total ≈ 1,203 deep-read notes (the 2026-08-12 Liang 2026 liver organoid fibrosis platform file is the net-new entry since the 2026-08-10 fetch). The 4 vendor subfolders (Celvivo / 3Brain / Cellink / Organoid) cover the canonical product KB per the wiki goal focus areas.
## Literature deep-read (LDR) pipeline
@@ -176,12 +176,13 @@ From [`/sources/ldr-report-template.md`](/sources/ldr-report-template.md) (Robin
| Miao 2025 Cell v2 (2026-08-08 flywheel-sync) | CelVivo (ClinoStar rating 5) | `/home/ldw/workspace/Miao2025_Cell_v2.md` | [`/sources/literature-miao-2025-cell-vascularization-v2-deepread.md`](/sources/literature-miao-2025-cell-vascularization-v2-deepread.md) |
| Liu 2026 Nat Biomed Eng (2026-08-08 flywheel-sync) | 3Brain (HD-MEA rating 5) + Cellink (microlattice rating 5) | `/home/ldw/workspace/Liu2026_NBME_shape_conformal_organoid.md` | [`/sources/literature-liu-2026-nbme-shape-conformal-organoid-deepread.md`](/sources/literature-liu-2026-nbme-shape-conformal-organoid-deepread.md) |
## Recent 30-day research output (mtime 2026-06-16 → 2026-08-10)
## Recent 30-day research output (mtime 2026-06-16 → 2026-08-12)
From [`/sources/literature.md`](/sources/literature.md) last sync, broken out by vendor and date:
| Date | DOI / slug | Vendor | Topic |
| --- | --- | --- | --- |
| 2026-08-12 | **Liang 2026 liver organoid fibrosis platform 4-commit LDR cycle** (commits `e542c977` + `8699a154` + `aacba7aa` + `297f106d`) | **CelVivo (ClinoStar rating 5 + ClinoReactor rating 5)** | Liang Y et al. (2026) A robust mouse liver organoid platform enables sustained multicellular maturation and fibrosis modeling from a single tissue sample (Sci Rep DOI 10.1038/s41598-026-42990-2, PMID 41857098; Wiki.js Id 1716, NocoDB Id 770; 3 轨道 Hep-Orgs + Cho-Orgs + HSC 同步分离; DAPT+Dex P1-P10 长程稳定成熟 [ALB/PAS/LDL/CYP 跨代保持]; TGFβ 激活 qHSC → aHSC [Acta2/Col1a1 上调]; aHSC 共培养诱导 Hep-Orgs EMT [Snail/Twist/Vimentin↑; ALB/CK8↓; Lgr5/EpCAM↓; Ki67↓] = 体外肝纤维化模型) — page 946 9 项 SOP 重写版 + page 934 修齐 标杆; 7/7 Ollama qwen2.5vl 视觉审核通过; SeaFile 7/7 公网 200 验真. First paper whose `related:` field already names `[[MOC-类器官与3D培养]]` (the 2026-08-10 first MOC) on the day of publication. |
| 2026-08-10 | **Phase A metadata batch + first MOC** (commits `d97b39ed` + `6b717aef` + `3b929c2f`) | (process discipline) | 6 deep-reads received the 6-field frontmatter (doi/journal/year/volume/pages/authors/pmid); first MOC `MOC-类器官与3D培养.md` (95 lines, 沙牛《AI 时代的 Obsidian 公开课》课时 1 pattern) created at `04-Literature/Deep-Reads/MOCs/`; 7 image alt descriptions added on `wang2024-org-on-chip.md`. No new external evidence. |
| 2026-08-09 | `10.7150/thno.90492` (Wang 2024) | (4 类共性硬件 — Organoid-on-Chip 综述) | Wang H et al. (2024) Human organoids-on-chips for biomedical research and applications (Theranostics 14(2):788-818, PMID 38169573; 7 图覆盖 7 大主题板块) — Wiki.js ID 1700 (v2 - grp:3 ghost page 累加) |
| 2026-08-08 | `10.3390/cells15110963` (Fernandez Vallone 2026) | (NAMs adjacent, hiPSC brain organoid) | THSDC chemical evaluation platform using hiPSC brain organoids (Cells, v2 5 张真图 + 双 H1; Wiki.js Id 1698) |
@@ -208,15 +209,15 @@ From [`/sources/literature.md`](/sources/literature.md) last sync, broken out by
By vendor (30-day mtime):
| Vendor | Most recent | 2026-08-08 / 2026-08-10 additions |
| Vendor | Most recent | 2026-08-08 / 2026-08-10 / 2026-08-12 additions |
| --- | --- | --- |
| CelVivo | 2026-08-08 (Miao 2025 v2 — ClinoStar rating 5) | +Miao 2025 v2 (ClinoStar co-differentiation), +Sharma 2026 (ClinoStar rating 3 adjacent), +Liu 2026 (ClinoStar rating 4 adjacent) |
| CelVivo | 2026-08-12 (Liang 2026 — ClinoStar rating 5 + ClinoReactor rating 5) | +Liang 2026 (P1-P10 长程成熟 + 单组织 3 轨道分通道灌流 + TGFβ 时间程序精确切换 — liver organoid fibrosis platform), +Miao 2025 v2 (ClinoStar co-differentiation), +Sharma 2026 (ClinoStar rating 3 adjacent), +Liu 2026 (ClinoStar rating 4 adjacent) |
| 3Brain | 2026-08-08 (Liu 2026 — HD-MEA rating 5) | +Liu 2026 (shape-conformal framework, next-gen HD-MEA), +Sharma 2026 (HD-MEA rating 4 adjacent), +Shin 2024 (Phase A frontmatter hygiene on existing note) |
| Cellink | 2026-07-27 (Tasevska 2025) | +Liu 2026 (microlattice fabrication rating 5 adjacent) |
| Adjacent (no vendor) | 2026-08-08 (Sharma 2026 retinal vascularization) | +Sharma 2026 (retinal organoid application domain) |
| (Process discipline) | 2026-08-10 (Phase A batch + first MOC) | +6 deep-reads received 6-field frontmatter; +first MOC `MOC-类器官与3D培养.md` created; +7 image alt descriptions on Wang 2024 — no new external evidence |
The pipeline is **vendor-balanced** — CelVivo, 3Brain, Cellink each have a recent deep read, and the 2026-08-08 flywheel-sync adds **3 papers spanning all 3 primary vendors + the retinal organoid application domain** (Sharma), all 3 rated 4-5 on at least one vendor's product line. The 2026-08-10 cluster is **process discipline** (Phase A metadata batch + first MOC creation + Wang 2024 alt-description sample), not new external evidence — the 6 deep-reads already existed on `main`; the 3 commits only normalize their metadata and add a knowledge-graph index layer. The **first entry in the new NocoDB table `Robin_Picked_Papers`** is Liu 2026 (created 2026-08-08 per Robin instruction to capture papers Robin hand-picks / provides outside the regular PubMed triage). The **first MOC** `MOC-类器官与3D培养.md` is the start of a Map-of-Content layer Robin appears to be building (the MOC's own `related:` already names `[[MOC-3Brain-HD-MEA]]` as the next planned MOC).
The pipeline is **vendor-balanced** — CelVivo, 3Brain, Cellink each have a recent deep read, and the 2026-08-08 flywheel-sync adds **3 papers spanning all 3 primary vendors + the retinal organoid application domain** (Sharma), all 3 rated 4-5 on at least one vendor's product line. The 2026-08-10 cluster is **process discipline** (Phase A metadata batch + first MOC creation + Wang 2024 alt-description sample), not new external evidence — the 6 deep-reads already existed on `main`; the 3 commits only normalize their metadata and add a knowledge-graph index layer. The 2026-08-12 cluster adds **net-new external evidence**: Liang 2026 Sci Rep liver organoid fibrosis platform (4-commit LDR cycle on a single paper, CelVivo ClinoStar ⭐⭐⭐⭐ + ClinoReactor ⭐⭐⭐⭐); this is the **first paper on `main` whose `related:` field already names `[[MOC-类器官与3D培养]]` on the day of publication**, confirming the MOC pattern is in active use on real deep-reads (vs the prior 2026-08-10 "MOC `related:` lists future MOCs" framing which was only prospective). The **first entry in the new NocoDB table `Robin_Picked_Papers`** is Liu 2026 (created 2026-08-08 per Robin instruction to capture papers Robin hand-picks / provides outside the regular PubMed triage). The **first MOC** `MOC-类器官与3D培养.md` is the start of a Map-of-Content layer Robin appears to be building (the MOC's own `related:` already names `[[MOC-3Brain-HD-MEA]]` as the next planned MOC).
## Sales ammunition pipeline
@@ -391,4 +392,4 @@ These are source-anchored but not vendor-anchored — they live here for traceab
| How does the agent stack consume this output? | [`/themes/03-ai-agents.md`](/themes/03-ai-agents.md) |
| How does the local-LLM fleet host this evidence? | [`/themes/04-local-llm.md`](/themes/04-local-llm.md) |
| Where does CelVivo + 3Brain output land? | [`/themes/01-organoid-equipment.md`](/themes/01-organoid-equipment.md) |
| Where does Cellink output land? | [`/themes/02-bioprinting.md`](/themes/02-bioprinting.md) |
| Where does Cellink output land? | [`/themes/02-bioprinting.md`](/themes/02-bioprinting.md) ||