chore: daily update 2026-08-25

.last-update.json

@@ -1,5 +1,5 @@
 {
-  "updatedAt": "2026-08-23T18:18:45.787Z",
+  "updatedAt": "2026-08-24T18:23:53.059Z",
   "command": "update",
   "model": "MiniMax-M3",
   "status": "complete",

commitments.md

@@ -123,6 +123,41 @@ Concrete work tasks, follow-ups, approvals, and scheduled items extracted from G
 - Status: open
 - Notes: **First customer-facing template produced from a single deep-read** in Robin's vault — the IRB application checklist is the canonical "Robin 培训客户 IRB 申请" core material for class A tertiary hospitals running organoid IRB submissions. The 7 mandatory points + 5 customer Q&A + China vs International flow table + 10 attachments + 7 citations map directly to the CelVivo ClinoStar clinical-translation sales narrative (Guo 2026 lists CelVivo ClinoStar ⭐⭐⭐⭐ for IRB compliance ammunition). **Three follow-ups needed**: (a) **commit** `templates/类器官IRB申请清单.md` to `main` (currently untracked); (b) add a wiki-side deep-read synthesis page at `/sources/literature-guo-2026-front-cell-dev-biol-organoid-ethics-deepread.md` mirroring the pattern at `/sources/literature-liu-2026-nbme-shape-conformal-organoid-deepread.md`; (c) fold the IRB checklist into the existing `05-Documents/Products/Celvivo/Industrial-Customers/` CelVivo commercial docs subtree (or, if the `templates/` folder convention holds, fold it into the new `templates/` vault subtree instead — see Robin decision). Companion commitment to fold into Chinese customer IRB conversations: the checklist covers China《人类遗传资源管理条例》+ 《涉及人的生命科学和医学研究伦理审查办法》2023 — directly applicable to the next Class A tertiary hospital Robin visits for CelVivo or 3Brain training.
 
+### celvivo-app-note-2026-clinostar-vs-orbital-shaker-positioning: fold the head-to-head shear-stress comparison into ClinoStar sales narrative + CelVivo赋能培训
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `fb017e4f` (CelVivo Application Note 2026 shear stress ClinoStar vs orbital shaker, Wiki.js 1747, sourced from RAGFlow CelVivo KB 1555e5b2..., Robin 8-24 上传; vault path `obsidian-vault/04-Literature/Deep-Reads/CelVivo_Shear_Stress_AppNote_2026.md`, 10,145 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **Canonical ClinoStar vs-orbital-shaker competitive-killer document** — the App Note establishes head-to-head comparison of ClinoStar® vs orbital shaker for 3D 类器官/球体培养 (HepG2/C3A 15-day + embedded Caco-2 肠 + brain organoid) with 3 bio-replicates per condition. Core findings: (a) **low shear stress ClinoStar preserves cytoskeletal integrity** — phalloidin/DAPI 染色 shows ClinoStar F-actin 排列规则 + 形态紧凑 + 细胞间内聚性强 vs orbital shaker F-actin 散布 + 形态松散 + 内聚性下降 (4x + 20x dual magnification); (b) **15-day standardized workflow** — same source cells + same pre-conditioning + same end-point assays; (c) **multi-model transferability** — embedded Caco-2 + brain organoid both show ClinoStar advantage → vendor claim "low shear stress sensitive cell class (肝/肠/脑来源)" validated across 3 model systems. **Highest commercial-relevance paper of the 2026-08-23 LDR cluster** — Robin's first **vendor App Note deep-read** (vs prior primary research + review only). Sales ammunition: "你的 orbital shaker 培养球体 F-actin 散布 + 内聚性下降 — ClinoStar 旋转培养保持细胞骨架完整 + 形态紧凑 — 15 天数据 + 3 bio-replicate 标准流程 + 3 类细胞模型 (HepG2 + Caco-2 + brain organoid)". Companion to existing `celvivo-clinoreactor-thewell-bioscience-xeno-free` commitment. Follow-ups: (a) fold the App Note into `06-Sales/Content/Training-Materials/` (the existing CelVivo赋能培训 v1-v9 cluster could absorb the head-to-head comparison data as a new PPT module); (b) add a wiki-side deep-read synthesis page at `/sources/literature-celvivo-shear-stress-appnote-2026-deepread.md`; (c) confirm with CelVivo whether this App Note is officially sanctioned for Robin's China customer use.
+
+### aiello-2025-rc-cfd-clinostar-positioning: fold the < 20 mPa quantitative CFD number into CelVivo ClinoStar sales narrative
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `f2118a62` (Aiello & Bagni 2025 biorxiv preprint Rotating Chamber brain organoid CFD, Wiki.js 1746, the EMBO Reports submission version `Aiello_Bagni_EMBO R_050325`, Robin 8-24 从 CelVivo KB 找到的 PDF; vault path `obsidian-vault/04-Literature/Deep-Reads/Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid.md`, 11,731 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **First CFD-quantified Rotating Chamber (RC) low-shear-stress study** in Robin's deep-read set — the paper quantifies what prior ClinoStar marketing material only described qualitatively. Core finding: **COMSOL Multiphysics 6.2 CFD simulation shows cavity shear stress < 20 mPa** (extremely low vs. standard stirred/shaken systems) — the **first quantitative number** for RC shear-stress field. Multi-density validation: relative density 1010/1020/1030 vs medium 1000 confirms shear-stress uniformity across density range + 0-300 objects all experience stable low shear. Morphological CV < 15% across batches + cell lines vs static culture CV > 30%. Cortical cell type recapitulation (SOX2/Nestin/PAX6 + DCX + TBR1/SATB2/MAP2 multi-marker IHC) + scRNA-seq OXPHOS metabolic profiling. **Vendor-anchor mapping**: Aiello paper is **CelVivo ClinoStar-adjacent** (uses RC system which is the ClinoStar simplified geometry) + 3Brain HD-MEA-adjacent (cortical organoid electrophysiology read-out). Sales ammunition: "你的类器官培养系统剪切力是多少? 静态培养 = 不均匀; orbital shaker = 不均匀; ClinoStar 旋转腔 = **< 20 mPa (CFD 量化)** + 跨密度一致 + 跨批次形态变异 < 15%". The < 20 mPa number is **direct ammunition** for the existing ClinoStar ⭐⭐⭐⭐ vendor-positioning pitch — no prior paper in Robin's deep-read set provides the quantitative CFD number. Follow-ups: (a) add a wiki-side deep-read synthesis page at `/sources/literature-aiello-2025-biorxiv-brain-organoid-fluid-deepread.md`; (b) fold the < 20 mPa number into the ClinoStar industrial-customers program alongside the Miao 2025 vHLPO + Liang 2026 liver organoid entries.
+
+### kim-2025-egain-3d-mea-vs-3brain-positioning: track the next-gen HD-MEA field consolidation (Liu 2026 Rogers Lab + Kim 2025 EGaIn + 3Brain Accura-3D)
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `5f02489a` (Kim E, Jeong E, Hong YM et al. 2025 Nat Commun liquid-metal 3D MEA, DOI 10.1038/s41467-024-55752-3, Wiki.js 1744; vault path `obsidian-vault/04-Literature/Deep-Reads/Kim_2025_NatCommun_Liquid_Metal_3D_MEA.md`, 13,567 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **3 distinct next-gen HD-MEA approaches now in Robin's deep-read set**: (a) **Liu 2026 Nat Biomed Eng** (Rogers Lab flexible-electronics shape-conformal framework, 3000-8000 channels per organoid, >90% surface coverage) — see existing `liu-2026-hd-mea-roadmap-implication` commitment; (b) **Kim 2025 Nat Commun** (EGaIn liquid-metal 3D pillar MEA, 25-310 μm pillar height, 14 μm diameter, parylene-C selective encapsulation, Ni magnetic layer for shape reconfiguration); (c) **3Brain Accura-3D** (rigid CMOS, 4096 electrodes, planar). The Kim paper is **3Brain HD-MEA competitor / adjacent paper** — Kim's EGaIn 3D MEA is a next-generation flexible 3D MEA approach that is **adjacent to but not directly competing with** 3Brain's rigid Accura-3D chip. Vendor-anchor mapping: Kim paper pairs with Liu 2026 to anchor the **next-gen HD-MEA field consolidation narrative** that Robin's existing `liu-2026-hd-mea-roadmap-implication` commitment tracks. Robin should pitch 3Brain's current 4096-electrode Accura-3D as the **existing best-in-class** AND track whether 3Brain has a flexible-electronics / EGaIn-3D-pillar framework on their roadmap. Follow-ups: (a) add a wiki-side deep-read synthesis page at `/sources/literature-kim-2025-nat-commun-liquid-metal-3d-mea-deepread.md`; (b) update the `liu-2026-hd-mea-roadmap-implication` commitment's Notes to reference Kim 2025 as the second corroborating evidence point (Liu + Kim = 2 distinct next-gen approaches); (c) verify with 3Brain whether the EGaIn / flexible-electronics / shape-conformal directions are on their 2026-2027 product roadmap.
+
+### ajongbolo-2025-brain-organoid-review-cluster: pair with Organoids 2026 review to anchor brain-organoid review lane
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `ad4abcde` (Ajongbolo AO, Langhans SA 2025 Cells brain organoid + assembloid review, DOI 10.3390/cells14110842, Wiki.js 1743; vault path `obsidian-vault/04-Literature/Deep-Reads/Ajongbolo_2025_Cells_Brain_Organoids_Review.md`, 11,367 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **Canonical brain organoid + assembloid review** for Robin's deep-read set — the paper establishes a **3-tier taxonomy** (whole-brain → region-specific → complex systems) + **4-class characterization methodology** (morphological + biochemical + functional + omics) + disease-modeling boundaries (neurodevelopmental / neurodegenerative / brain-tumor) + drug-discovery HTS pipeline. **First comprehensive brain-organoid review** in Robin's deep-read set since the 2026-07-29 Organoids 2026 review (`10.1186_s40164-026-00743-x`, vault path `04-Literature/Deep-Reads/Organoid/2026-07-29_Organoids_disease_modeling_treatment_2026_review.md`). Pairs with that review to anchor the **brain-organoid review-paper lane**. Vendor-anchor mapping: **CelVivo ClinoStar-adjacent** (long-term stable brain organoid culture) + 3Brain HD-MEA-adjacent (functional electrophysiology read-out via MEA in the 4-class characterization methodology). Follow-ups: (a) add a wiki-side deep-read synthesis page at `/sources/literature-ajongbolo-2025-cells-brain-organoids-review-deepread.md`; (b) cross-link the Ajongbolo synthesis page with the existing Organoids 2026 review entry on `/themes/05-robin-research.md` review-paper cluster; (c) consider promoting the brain-organoid review lane to a `/themes.md` row if a 2026-08-25 fetch surfaces another brain-organoid paper.
+
+### tian-2025-epicardium-mtor-cardiac-expansion: track cardiac epicardium lane for potential CDMO/regenerative-medicine opportunities
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `40b357b8` (Tian Y, Lucena-Cacace A, Tani K et al. 2025 Nat Commun hiPSC epicardium mTOR maturation, DOI 10.1038/s41467-025-60934-8, Wiki.js 1745; vault path `obsidian-vault/04-Literature/Deep-Reads/Tian_2025_NatCommun_Epicardium_mTOR.md`, 15,578 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **First cardiac-epicardium paper in Robin's deep-read set** — Tian paper establishes **Torin1 (dual mTORC1/2 inhibitor, 200 nM) drives hiPSC epicardium maturation** with mature markers (TJP1/WT1/TBX18/ALDH1A2/CDH18) up + EMT markers down + proliferation markers down + fatty-acid metabolism maturation; 379 DEGs; adult-like profile vs GSE84085; YBX3 + MAFF identified as Torin1-specific TFs; **paracrine TNNI3 upregulation in hiPSC ventricular cardiomyocyte EBs co-cultured for 10 days** (epicardium-to-cardiomyocyte maturation cross-talk). **Application domain**: cardiac organoid regeneration — fits the broad cardiac application-domain bucket that Robin's wiki scope covers. **No vendor callouts in the deep-read** (no CelVivo / 3Brain / Cellink / FemtoBiomed product rating) but the paracrine cross-talk finding is a candidate for cross-vendor positioning if Robin pitches cardiac-regeneration programs to pharma R&D or CDMO accounts. The 15,578-byte size is the **largest** of the 5 new LDR files — reflects Robin's deep-dive on the mTOR pathway mechanism. Follow-ups: (a) add a wiki-side deep-read synthesis page at `/sources/literature-tian-2025-nat-commun-epicardium-mtor-deepread.md`; (b) verify whether any active Chinese pharma R&D / CDMO / regenerative-medicine account maps to the cardiac-epicardium lane (the active CRM profile set is mostly CelVivo / 3Brain neural-organoid focused — no cardiac-epicardium customer dossier surfaced yet); (c) consider whether Robin should fold cardiac into the existing `/themes/01-organoid-equipment.md` theme or open a new `theme-cardiac-regeneration.md` if cardiac papers recur.
+
 <!--
 Structure for new entries:
 

index.md

@@ -8,7 +8,7 @@ okf_version: "0.1"
 - [2026-07-16 工具链打通战报](memory-2026-07-16-tools-final.md) - After-action report on Robin's 2026-07-16 end-to-end toolchain integration: OpenWiki + Gitea + NocoDB + RAGFlow + Wiki.js + MiniMax code all wired together and working in production. Snapshot of the pre-flywheel-sync state — pre-dates the 2026-07-26 HN + Tavily launches and the 2026-07-29 Tavily cluster / 2026-08-08 literature flywheel-sync milestones.
 - [Open Questions](open-questions.md) - Active, Answered, and Stale unresolved questions about Robin's memory, recurring routines, people, projects, and wiki quality. Source-document TODOs and product questions live on their source pages instead.
 - [Personal Logistics](personal-logistics.md) - Personal errands, appointments, pickups, travel, household/life-admin deadlines, and other non-work logistics. Work commitments live in /commitments.md.
-- [Robin's Local Knowledge Base](quickstart.md) - OpenWiki entrypoint and orientation — Robin's personal knowledge base with 5 synthesis themes (organoid equipment, bioprinting, AI agents, local LLM, personal research), navigation map, current connector status, and backlog.
+- [Robin's Local Knowledge Base](quickstart.md) - OpenWiki entrypoint and orientation — Robin's personal knowledge base with 5 synthesis themes (organoid equipment, bioprinting, AI agents, local LLM, personal research), navigation map, current connector status, and backlog. The 2026-08-24 18:05 UTC fetch surfaced the largest single-day LDR burst since 2026-07-29 — 5 papers committed to the Obsidian vault main + new pipeline-workspace directory `deepread_40240593/`.
 - [OpenWiki 现状 + 操作手册 (2026-07-27)](README-ACTIVATION.md) - Activation status + operations manual for Robin's local OpenWiki knowledge base as of 2026-07-27. Documents 4 active connectors (HN + git-repo + Tavily + RAGFlow) and the per-source sync scripts (HN top+best feeds, Tavily web-search with TAVILY_API_KEY, git-repo Obsidian vault at /home/ldw/workspace, RAGFlow full-text retrieval at 10.10.10.254:9980). Captures the on-disk Gitea commit + push pattern.
 - [Themes](themes.md) - Recurring topics, signals, and trends observed across Robin's sources. Index of theme rows with confidence labels and source citations; details live on the linked source and synthesis pages.
 

quickstart.md

@@ -1,9 +1,9 @@
 ---
 type: "Reference"
 title: "Robin's Local Knowledge Base"
-description: "OpenWiki entrypoint and orientation — Robin's personal knowledge base with 5 synthesis themes (organoid equipment, bioprinting, AI agents, local LLM, personal research), navigation map, current connector status, and backlog."
-tags: [quickstart, navigation, openwiki, status, 2026-08-23, 2026-08-22]
-timestamp: 2026-08-23T18:11:00Z
+description: "OpenWiki entrypoint and orientation — Robin's personal knowledge base with 5 synthesis themes (organoid equipment, bioprinting, AI agents, local LLM, personal research), navigation map, current connector status, and backlog. The 2026-08-24 18:05 UTC fetch surfaced the largest single-day LDR burst since 2026-07-29 — 5 papers committed to the Obsidian vault main + new pipeline-workspace directory `deepread_40240593/`."
+tags: [quickstart, navigation, openwiki, status, 2026-08-24, 2026-08-23, 2026-08-22]
+timestamp: 2026-08-24T18:05:00Z
 ---
 
 # Robin's Local Knowledge Base
@@ -34,9 +34,11 @@ timestamp: 2026-08-23T18:11:00Z
 
 ## Current status
 
-- **Wiki state:** initialized — Hacker News, the local Obsidian vault, and Tavily web search are active and feeding `/sources/hackernews.md`, `/sources/git-repo.md`, `/sources/web-search.md`, and `/themes.md`. All 5 synthesis themes (`/themes/01-organoid-equipment.md`, `/themes/02-bioprinting.md`, `/themes/03-ai-agents.md`, `/themes/04-local-llm.md`, `/themes/05-robin-research.md`) are now generated; the 2026-08-17 daily update created `/sources/openwiki-version-drift.md` (auto-generated OpenWiki version-drift monitor); the 2026-08-23 daily update committed the synthesis-layer edits from the prior HN-1 2026-08-22T18:00Z + web-search-1 2026-08-22 (skipped) + web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-22T18:13Z runs; the wiki HEAD bumped to `a9568d8` (sources-sync 2026-08-23, the **ninth** consecutive sources-sync re-applying the `2507925` 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + now `8f8f767` 2026-08-23). The 6 uncommitted synthesis-layer edits re-appear in the working tree because the very next synthesis edits from this git-repo-1 2026-08-23T18:11Z run re-touch the same files. **2026-08-23 18:11 UTC update**: git-repo-1 (Obsidian vault + OpenWiki wiki) ran; vault HEAD unchanged at `a4fa5a32` (vault silent for the **7th consecutive fetch**, **7 calendar days stale** since the 2026-08-16 Quintard + Guo cluster); wiki HEAD bumped `43d4548` → `a9568d8` via 2 automated wiki commits (`a9568d8` daily update 2026-08-23 + `8f8f767` sources-sync 2026-08-23 — the **ninth** consecutive sources-sync); synthesis layer (themes / commitments / open-questions / personal-logistics) is independent of the wiki-side automated maintenance commits and is unchanged this run.
+- **Wiki state:** initialized — Hacker News, the local Obsidian vault, and Tavily web search are active and feeding `/sources/hackernews.md`, `/sources/git-repo.md`, `/sources/web-search.md`, and `/themes.md`. All 5 synthesis themes (`/themes/01-organoid-equipment.md`, `/themes/02-bioprinting.md`, `/themes/03-ai-agents.md`, `/themes/04-local-llm.md`, `/themes/05-robin-research.md`) are now generated; the 2026-08-17 daily update created `/sources/openwiki-version-drift.md` (auto-generated OpenWiki version-drift monitor); the 2026-08-24 daily update committed the synthesis-layer edits from the prior HN-1 2026-08-23T18:00Z + web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-23T18:11Z runs; the wiki HEAD bumped to `127c2dc` (sources-sync 2026-08-24, the **tenth** consecutive sources-sync re-applying the `2507925` 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + `8f8f767` 2026-08-23 + now `127c2dc` 2026-08-24). The 8 uncommitted synthesis-layer edits re-appear in the working tree because the very next synthesis edits from this git-repo-1 2026-08-24T18:05Z run re-touch the same files. **2026-08-24 18:05 UTC update**: git-repo-1 (Obsidian vault + OpenWiki wiki) ran; **vault HEAD bumped** `a4fa5a32` → `fb017e4f` via **5 new vault commits** (the largest single-day LDR burst since 2026-07-29: Ajongbolo 2025 Cells brain organoid review + Kim 2025 Nat Commun EGaIn 3D MEA + Tian 2025 Nat Commun hiPSC epicardium mTOR + Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid CFD + CelVivo App Note 2026 shear stress ClinoStar vs orbital shaker — all 5 landed at `04-Literature/Deep-Reads/` root, non-standard pattern); wiki HEAD bumped `a9568d8` → `127c2dc` via 2 automated wiki commits (`9cc5e63` daily update 2026-08-24 + `127c2dc` sources-sync 2026-08-24 — the **tenth** consecutive sources-sync); 5 new commitments added to `/commitments.md` (one per new LDR paper); 5 new rows added to `/sources/literature.md` "Last 30 days of deep reads" table; `/themes.md` `class-organoid-3d-bioprinting` evidence footprint strengthened (3 of 5 papers vendor-aligned to CelVivo + 3Brain) but no new theme row at this fetch.
 - **Connectors:** Hacker News, git-repo, and web search active. The 2026-08-08, 2026-08-10, 2026-08-12, 2026-08-15, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, and 2026-08-23 web-search update attempts were skipped because the connector is not enabled and `TAVILY_API_KEY` is empty; the latest successful Tavily pull remains 2026-08-03 18:08 UTC. Gmail, Slack, Notion, and X are not yet configured — see "Adding sources" below.
 
+**Latest update — git-repo-1 (2026-08-24T18:05Z).** One source-specific update run for `git-repo-1` (Obsidian vault + OpenWiki wiki) per the source-specific instruction "Track vault + OpenWiki wiki mtime + commits (Robin 8-07 added)". Fresh connector ingest ran via `openwiki_ingest_connector connectorId=git-repo` and wrote manifest at `~/.openwiki/connectors/git-repo/raw/2026-08-24T18-05-31-919Z/manifest.json`. **Vault HEAD bumped** from `a4fa5a323d6dcabeb9eccad49e636ee6ba5f87a6` (2026-08-23T18:11Z fetch) to **`fb017e4fb40f874e54fb2a92eff235abe1c487cb`** (2026-08-24 +0800) — **5 new vault commits** in the 2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window: (a) `ad4abcde` 2026-08-24 feat: Ajongbolo 2025 Cells 脑类器官综述精读 (Wiki.js 1743) → adds `obsidian-vault/04-Literature/Deep-Reads/Ajongbolo_2025_Cells_Brain_Organoids_Review.md` (11,367 bytes); (b) `5f02489a` 2026-08-24 feat: Kim 2025 NatCommun 液态金属 3D MEA 精读 (Wiki.js 1744) → adds `obsidian-vault/04-Literature/Deep-Reads/Kim_2025_NatCommun_Liquid_Metal_3D_MEA.md` (13,567 bytes); (c) `40b357b8` 2026-08-24 feat: Tian 2025 NatCommun 心外膜 mTOR 精读 (Wiki.js 1745) → adds `obsidian-vault/04-Literature/Deep-Reads/Tian_2025_NatCommun_Epicardium_mTOR.md` (15,578 bytes, largest of the 5); (d) `f2118a62` 2026-08-24 feat: Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid 精读 (Wiki.js 1746) → adds `obsidian-vault/04-Literature/Deep-Reads/Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid.md` (11,731 bytes); (e) `fb017e4f` 2026-08-24 feat: CelVivo App Note 2026 shear stress ClinoStar vs orbital shaker (Wiki.js 1747) → adds `obsidian-vault/04-Literature/Deep-Reads/CelVivo_Shear_Stress_AppNote_2026.md` (10,145 bytes). **All 5 files landed at `obsidian-vault/04-Literature/Deep-Reads/` root** (not in a per-vendor subfolder — **non-standard pattern** vs the prior per-vendor subfolder discipline). All 5 are single-shot LDR entries (no v2/v3 SOP rewrite cycles, unlike the 2026-08-16 Quintard 4-commit + Guo 3-commit pattern). Vendor mapping: **3 CelVivo-aligned** (CelVivo App Note + Aiello [ClinoStar-adjacent] + Ajongbolo review mentions ClinoStar) + **1 3Brain-adjacent** (Kim EGaIn 3D MEA — 3rd next-gen HD-MEA approach alongside Liu 2026 + 3Brain Accura-3D) + **1 cardiac** (Tian epicardium mTOR, no vendor callouts). **Largest single-day LDR burst since the 2026-07-29 Parate / Lee / Hodge cluster** + **breaks the 7-fetch vault-silence streak** that started after the 2026-08-16 Quintard + Guo cluster. **1 new untracked directory** appeared in the working tree this fetch — `deepread_40240593/` (7 subdirs: 5 PMID-named `deepread_<PMID>/` + 2 slug-named `deepread_brain_organoid_fluid/` + `deepread_shear_stress_cs_os/` that exactly map to the Aiello + CelVivo App Note papers; the new directory is Robin's LDR pipeline intermediate-artifact workspace, see `/sources/git-repo.md` Notable-entity § "deepread_40240593/ pipeline workspace"). The 2 prior untracked directories (`celvivo_commercial/` + `templates/`) and the `Review/zhou2023_computer_vision_microfluidics.md` first-entry-in-new-`Review/`-subfolder file at **13-day carry-forward** since 2026-08-11 (mtime `2026-08-11 16:31:54 +0800`), uncommitted since then, **11 fetches silent** on the file. **Wiki HEAD** bumped from `a9568d86d5c4567db4b9b35241e9b6b89995a66b` (2026-08-23T18:11Z fetch) to **`127c2dc60fb8398d02485313df25994e7e18c69f`** (2026-08-24T02:30 +0800, the 2026-08-24 sources-sync); **2 new automated wiki commits** in the 24-hour window: `9cc5e63` chore: daily update 2026-08-24 (2026-08-24T02:23 +0800 — carried the synthesis-layer edits from the prior HN-1 2026-08-23T18:00Z + web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-23T18:11Z runs; committed `sources/git-repo.md` + `sources/hackernews.md` + `sources/index.md` + `sources/openwiki-version-drift.md` + `sources/web-search.md` + `themes/05-robin-research.md` from the prior dirty set) + `127c2dc` chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-24, 2026-08-24T02:30 +0800 — the **tenth** consecutive sources-sync re-applying the `2507925` 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + `8f8f767` 2026-08-23 + now `127c2dc` 2026-08-24). **8 uncommitted wiki files** in working tree per the manifest `changedFiles` list (`.last-update.json` + `quickstart.md` + `sources/README.md` + `sources/git-repo.md` + `sources/hackernews.md` + `sources/literature.md` + `sources/openwiki-version-drift.md` + `sources/web-search.md`) — the `9cc5e63` daily update committed 6 of the prior 6 dirty files but the very next synthesis edits from this git-repo-1 2026-08-24T18:05Z run re-touch the same 6 files + add 2 newly dirty files `sources/literature.md` + `sources/git-repo.md`. Wiki edits this run: (a) `/sources/git-repo.md` — refreshLine truncated
+
 **Latest update — git-repo-1 (2026-08-23T18:11Z).** One source-specific update run for `git-repo-1` (Obsidian vault + OpenWiki wiki) per the source-specific instruction "Track vault + OpenWiki wiki mtime + commits (Robin 8-07 added)". Fresh connector ingest ran via `openwiki_ingest_connector connectorId=git-repo` and wrote manifest at `~/.openwiki/connectors/git-repo/raw/2026-08-23T18-11-34-883Z/manifest.json`. **Vault HEAD unchanged** at `a4fa5a323d6dcabeb9eccad49e636ee6ba5f87a6` (same as the 2026-08-17T18:09Z + 2026-08-18T18:10Z + 2026-08-19T18:08Z + 2026-08-20T18:09Z + 2026-08-21T18:09Z + 2026-08-22T18:13Z + 2026-08-23T18:11Z fetches — **vault is silent for the 7th consecutive fetch**, **7 calendar days stale** since the last vault HEAD bump on 2026-08-16) — **0 new vault commits** in the 2026-08-22T18:13Z → 2026-08-23T18:11Z 24-hour window; the 2026-08-16 Quintard 2024 Nat Commun 4-commit LDR cycle + Guo 2026 Front Cell Dev Biol 3-commit LDR cycle remain the most recent vault activity. **0 new vault mtime changes** — the 25+ untracked vault files + 2 untracked dirs from the 2026-08-17T18:09Z fetch carry forward unchanged (including `celvivo_commercial/1331651_20260630_131201.pdf` last touched 2026-08-17 15:04 +0800 = 07:04 UTC, **before** the 24h window + `templates/类器官IRB申请清单.md` last touched 2026-08-16 10:27 +0800 = 02:27 UTC, well before the window + the `Review/zhou2023_computer_vision_microfluidics.md` first-entry-in-new-`Review/`-subfolder file at **12-day carry-forward** since 2026-08-11 (mtime `2026-08-11 16:31:54 +0800`), uncommitted since then, **10 fetches silent** on the file). **Wiki HEAD** bumped from `43d4548` (2026-08-22T18:13Z fetch) to **`a9568d86d5c4567db4b9b35241e9b6b89995a66b`** (2026-08-23T02:29 +0800, the 2026-08-23 daily update); **2 new automated wiki commits** in the 24-hour window: `8f8f767` chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-23, 2026-08-23T02:30 +0800 — the **ninth** consecutive sources-sync re-applying the `2507925` 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + now `8f8f767` 2026-08-23) + `a9568d8` chore: daily update 2026-08-23 (2026-08-23T02:29 +0800 — carried the synthesis-layer edits from the prior HN-1 2026-08-22T18:00Z + web-search-1 2026-08-22 (skipped) + web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-22T18:13Z runs; committed 7 of the prior 9 dirty files including `sources/git-repo.md` + `sources/index.md` + `sources/nocodb.md` + `sources/ragflow.md` + `sources/seafile.md` + `sources/wikijs.md` + `themes/05-robin-research.md`, reducing the dirty count from 9 → 6). Both commits are routine auto-orchestration: (a) `a9568d8` carried the synthesis-layer edits from the prior HN-1 2026-08-22T18:00Z + web-search-1 2026-08-22 (skipped) + web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-22T18:13Z runs; (b) `8f8f767` re-applied the count-data wipe pattern on NocoDB / SeaFile / Wiki.js / RAGFlow for the **ninth** consecutive time. **6 uncommitted wiki files** in working tree (carry forward from the prior `a9568d8` 2026-08-23 daily update — the very next synthesis edits from this git-repo-1 2026-08-23T18:11Z run re-touch the same files): `.last-update.json` + `quickstart.md` + `sources/README.md` + `sources/hackernews.md` + `sources/openwiki-version-drift.md` + `sources/web-search.md`. Wiki edits this run: (a) `/sources/git-repo.md` — refreshed frontmatter HEAD pointers (vault HEAD unchanged at `a4fa5a32`, wiki HEAD `43d4548` → `a9568d8`, tags + `2026-08-23`, timestamp → `2026-08-23T18:11:00Z`), refreshed the Connector / State section to the new `2026-08-23T18-11-34-883Z` raw manifest, refreshed the obsidian-vault working-tree-status section to 2026-08-23 (0 new commits, 0 new mtime changes, untracked set unchanged; `Review/` 11-day → **12-day carry-forward** + `templates/` + `celvivo_commercial/` 6-day → **7-day carry-forward**), refreshed the openwiki-wiki working-tree-status section to 2026-08-23 (2 new automated wiki commits + the carry-forward 6 uncommitted synthesis-layer edits, down from 9 at the prior fetch because `a9568d8` committed 7 of the 9 dirty files), added 2 new entries at the top of the Recent-commits-for-wiki table (`a9568d8` daily update 2026-08-23 + `8f8f767` sources-sync 2026-08-23), refreshed the Summary-of-last-48-hours table to add the 2026-08-22T18:13Z → 2026-08-23T18:11Z window data (0 vault commits / 2 wiki commits / operational row noting the **ninth** consecutive sources-sync re-applying the count-data wipe pattern), refreshed the Notable-entity `Review/` entry (11-day → **12-day carry-forward**, **9 fetches silent** → **10 fetches silent**) + bumped `templates/` + `celvivo_commercial/` 6-day → **7-day carry-forward** + refreshed the `04-Literature/Deep-Reads/` breakdown table likewisLine truncated
 
 **Latest update — web-search-1 attempted 2026-08-23 skipped.** One source-specific update run for `web-search-1` (Web Search / Tavily) per the source-specific instruction "Track CelVivo / 3Brain / Cellink / organoid news from web (NOTE: needs TAVILY_API_KEY, currently empty)". The deterministic pull was **skipped**: `web-search-1` is not enabled in the runtime registry and `TAVILY_API_KEY` is empty, so no Tavily request was made and no raw data files were written. The nightly searxng-fallback slot `~/.openwiki/connectors/web-search/raw/2026-08-22T18-35-03-searxng-fallback/` is also empty (confirmed empty on disk — no files in the directory) — no web-search evidence of any kind landed in the 24-hour window. This is the **eleventh consecutive skip** on the same code path (after 2026-08-08 + 2026-08-10 + 2026-08-12 + 2026-08-15 + 2026-08-17 + 2026-08-18 + 2026-08-19 + 2026-08-20 + 2026-08-21 + 2026-08-22); the latest successful Tavily pull remains 2026-08-03 18:08 UTC. No new CelVivo / 3Brain / Cellink / organoid findings were available for synthesis; no changes were made to `/themes.md`, `/commitments.md`, `/open-questions.md`, or `/personal-logistics.md` from this source attempt. Discord between on-disk `~/.openwiki/connectors/web-search/config.json` (which shows `enabled: true` + 6 Chinese queries) and the runtime registry (still reports the connector as disabled) is the same operational issue noted on the 2026-08-08 / 2026-08-10 / 2026-08-12 + 2026-08-15 / 2026-08-17 / 2026-08-18 + 2026-08-19 / 2026-08-20 / 2026-08-21 + 2026-08-22 runs — recorded for traceability only, not a wiki-synthesis concern. Wiki edits this run: (a) `/sources/web-search.md` — added a "2026-08-23 attempted update — skipped" section ahead of the 2026-08-22 entry, following the established 5-field structure (Status / Authentication / Coverage / Synthesis / Operational note); updated the header line to mention the eleventh skip; refreshed the 2026-08-22 entry's "Operational note" to mention the 2026-08-23 follow-up; refreshed the 2026-08-21 entry's "Operational note" to mention the 2026-08-22 + 2026-08-23 follow-ups; refreshed the 2026-08-20 entry's "Operational note" to mention the 2026-08-21 + 2026-08-22 + 2026-08-23 follow-ups; refreshed the 2026-08-19 entry's "Operational note" to mention the 2026-08-20 + 2026-08-21 + 2026-08-22 + 2026-08-23 follow-ups; refreshed the 2026-08-18 entry's "Operational note" to mention the 2026-08-19 + 2026-08-20 + 2026-08-21 + 2026-08-22 + 2026-08-23 follow-ups; refreshed the 2026-08-17 entry's "Operational note" to mention the 2026-08-18 + 2026-08-19 + 2026-08-20 + 2026-08-21 + 2026-08-22 + 2026-08-23 follow-ups; refreshed the 2026-08-15 entry's "Operational note" to mention the 2026-08-17 + 2026-08-18 + 2026-08-19 + 2026-08-20 + 2026-08-21 + 2026-08-22 + 2026-08-23 follow-ups; added the 2026-08-23 cumulative coverage line + updated the header timestamp; added `2026-08-23` to the `tags` list; (b) `/sources/README.md` — refreshed Active connectors table + Connector roll table to reflect the 11-entry skip streak; (c) `/quickstart.md` — added this "Latest update — web-search-1 attempted 2026-08-23 skipped" paragraph; updated the Web Search connector row in "Current status" and "Adding sources" to reflect the 11-entry skip streak; updated the Backlog web-search entry likewise. **No new theme row, no commitment row, no open-question row, no personal-logistics item** — the synthesis layer is unchanged because the connector produced no new evidence. Prior run was **git-repo-1 update (2026-08-23T18:11Z)** — see previous paragraph.
@@ -121,12 +123,9 @@ Deferred until Robin configures connectors or asks for specific documentation:
 - **Slack evidence index** — `/sources/slack.md` (no Slack connector configured yet)
 - **Notion evidence index** — `/sources/notion.md` (no Notion MCP configured yet)
 - **X / Twitter evidence index** — `/sources/x.md` (no X connector configured yet)
-- **Hacker News evidence index** — `/sources/hackernews.md` (active; last refreshed 2026-08-22 18:00 UTC — source-specific LLM-agent topic set (`organoid`, `CelVivo`, `3Brain`, `Cellink`, `openwiki`, `deepagents`); **eleventh pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries** (after the 2026-08-08 03:15 + 2026-08-08 18:00 + 2026-08-13 + 2026-08-15 + 2026-08-16 + 2026-08-18 + 2026-08-19 + 2026-08-21 + 2026-08-22 fully-zero pulls + the 2026-08-10 + 2026-08-12 + 2026-08-17 + 2026-08-20 noise-only pulls; **2026-08-22 is the third fully-zero Algolia lane in a row**, after 2026-08-21 + 2026-08-19, but **the 2026-08-22 pull reverts to the noise-only shape** — substring-noise on `organoid` / `3Brain` / `Cellink` (5 + 6 + 3 = 14 hits) + clean zero on `CelVivo` / `openwiki` / `deepagents` — so 2026-08-22 is **NOT** the third fully-zero Algolia lane in a row) — vendor-anchored footprint remains null on the HN lane and Tavily (web-search) remains the productive lane; the 2026-08-20 pull (prior-prior) produced the `class-organoid-3d-bioprinting` theme-row Last-seen refresh 2026-08-12 → 2026-08-20 via the recurrence criterion (the `49366519` Nature organoid-time-passage paper 18 pts + `49373812` Science Alert cross-walk — second neural-organoid HN signal in 9 pulls), and that refresh carries forward unchanged into the 2026-08-22 pull (no organoid / 3D-bioprinting item this pull); **five named-stack-adjacent items flagged for traceability this pull, none promoting a `/themes.md` row**: **`49398152` "Munder Difflin – Agent harness to run an office of your clones"** (192 pts, 81 cmts top, by `simonpure` 2026-08-21, https://munderdiffl.in/ — agent harness for running an office of agent-clones; **strongest named-stack-adjacent item of the pull by direct agent-harness cross-walk** by virtue of (a) 192-pt + 81-cmt top-feed engagement, the third-highest top-feed engagement on the pull; (b) the **agent-harness "office of your clones" framing** cross-walks to the same agent-as-primary-compute-substrate narrative that Robin's `hermes-agent-v0-19-quicksilver` row documents; (c) **5th entry in the now 5-day self-hosted-agentic-substrate cluster** — DeepSeek Harness `49285244` 422 pts 2026-08-13 → machine0 `49348136` 19 pts 2026-08-18 → OneCLI `49363710` 21 pts 2026-08-19 → self-hosted agentic software factory `49390463` 16 pts 2026-08-21 → **Munder Difflin `49398152` 192 pts 2026-08-22** — 5 independent submissions across 5 consecutive working days all corroborating the same narrative, with Munder Difflin's 192-pt engagement being the second-highest of the cluster (after the 422-pt DeepSeek Harness) and **stronger than machine0 + OneCLI + self-hosted factory combined**; **adjacent-not-promoted** per scope because Munder Difflin is **not on Robin's named daily-driver stack** and the established scope rule keeps non-named-stack agent-harness launches adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself; **the cluster is now an emerging-pattern watchlist candidate** with 5 independent submissions across 5 consecutive working days); **`49399591` "New MCP Roadmap"** (120 pts, 93 cmts top, by `pentagrama` 2026-08-21, https://blog.modelcontextprotocol.io/posts/mcp-roadmap/ — official **MCP roadmap from the canonical MCP blog**; **strongest protocol-substrate signal of the pull** because (a) **MCP is the protocol-layer substrate of every named-stack item on Robin's workflow** — Hermes Agent (the `hermes-agent-v0-19-quicksilver` row cites the 2026-07-28 MCP revision finalization), Codex CLI, OpenWiki, RAGFlow (via MCP connectors), the machine0 + OneCLI self-hosted-agentic-substrate cluster entries; (b) **the MCP roadmap is the first MCP-protocol roadmap entry since the 2026-07-28 MCP revision finalization** documented on the `hermes-agent-v0-19-quicksilver` row; (c) the **120-pt + 93-cmt top-feed engagement** is moderate-to-high; (d) the MCP roadmap directly affects the substrate that `agent-memory-architecture` row's MCP Memory entry (the 2026-08-13 OKF+memory schema refresh) sits on; **adjacent-not-promoted** per scope because MCP is the **substrate protocol, not a named-stack vendor itself** and the established scope rule keeps substrate-protocol roadmap entries adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself; **the MCP substrate is its own substrate-cluster watchlist candidate** — MCP Memory 46 pts 2026-08-13 + machine0 MCP support 2026-08-18 + OneCLI MCP connectors 2026-08-19 + **New MCP Roadmap 120 pts 2026-08-22** — 4 distinct substrate-protocol signals across 4 pulls in 10 days); **`49401549` "Anthropic appears to be A/B testing reduced effort levels in Claude Code"** (29 pts, 17 cmts top, by `matthieu_bl` 2026-08-22, https://twitter.com/argofowl/status/2091150597374537729 — Twitter screenshot of Anthropic's apparent Claude Code effort-level A/B test; **4th CLine truncated
-- **Web search evidence index** — `/sources/web-search.md` (active; last successful pull 2026-08-03 18:08 UTC — first pull after the 3-day outage streak; CELLINK × Volumetric new Lumen X + 3Brain Utrecht Summer School + CelVivo ClinoStar 2 / Thailand Lab 2026 surfaced, see source page; 2026-08-08, 2026-08-10, 2026-08-12, 2026-08-15, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, and 2026-08-23 update attempts skipped — connector not enabled in runtime registry, `TAVILY_API_KEY` missing; disk config shows `enabled: true` + 6 Chinese queries but the runtime registry disagrees — operational concern, not a wiki-synthesis concern; **eleventh consecutive skip on the same code path** including 2026-08-23)
-- **Git repo evidence index** — `/sources/git-repo.md` (active; last refreshed 2026-08-22 18:13 UTC — covers Robin's Obsidian vault (HEAD `a4fa5a32` 2026-08-16, **0 new vault commits + 0 new mtime changes since the 2026-08-21T18:09Z fetch**; vault is silent in the 24-hour window for the **6th consecutive fetch**; the 2026-08-16 Quintard 2024 + Guo 2026 cluster remains the most recent vault activity, **6 calendar days stale**; the `Review/zhou2023_computer_vision_microfluidics.md` first-entry-in-new-`Review/`-subfolder is at **11-day carry-forward** (mtime `2026-08-11 16:31:54 +0800`), uncommitted since 2026-08-11, structural signal continues to weaken, **9 fetches silent** on the file) + OpenWiki wiki (HEAD `43d4548` 2026-08-22, **2 new automated wiki commits since the 2026-08-21T18:09Z fetch**: `d8e2277` chore: daily update 2026-08-22 (2026-08-22T02:23 +0800 — carried the synthesis-layer edits from the prior HN-1 2026-08-22T18:00Z + web-search-1 2026-08-22 (skipped) + git-repo-1 2026-08-21T18:09Z runs) + `43d4548` chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-22, 2026-08-22T02:30 +0800, the **eighth** consecutive sources-sync re-applying the `2507925` 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + now `43d4548` 2026-08-22) + the prior 2026-08-21 fetch's 2 wiki commits (`b6dc337` daily update 2026-08-21 + `f6c2576` sources-sync 2026-08-21) + the prior 2026-08-20 fetch's 2 wiki commits (`a44f284` daily update 2026-08-20 + `bd30339` sources-sync 2026-08-20) + the prior 2026-08-19 fetch's 2 wiki commits (`7e904ec` daily update 2026-08-19 + `dcb53a2` sources-sync 2026-08-19) + the prior 2026-08-17 fetch's 4 wiki commits (`151d22b` daily update 2026-08-16 + `997b648` sources-sync 2026-08-16 + `5c70881` daily update 2026-08-17 + `be49285` sources-sync 2026-08-17) + **1 new wiki file from the 2026-08-17 fetch**: `sources/openwiki-version-drift.md` (auto-generated OpenWiki version-drift monitor; v0.3.1 local → v0.3.3 npm latest as of 2026-08-15T20:00:03Z); **20 vault commits since 2026-08-03 fetch** (unchanged across the 2026-08-17 + 2026-08-18 + 2026-08-19 + 2026-08-20 + 2026-08-21 + 2026-08-22 fetches); **25+ untracked vault files + 2 untracked directories** carry forward unchanged from the 2026-08-17T18:09Z fetch (including 8 × `02-Clients/Agents/区域-2026-0807.md` distributors-rebuild + `_known_associations.md` association index + the 11-day carry-forward `Review/zhou2023_computer_vision_microfluidics.md` + `__pycache__/...pyc` Python build noise + `celvivo_commercial/1331651_20260630_131201.pdf` (**6-day carry-forward**) + `templates/类器官IRB申请清单.md` v1.0 (**6-day carry-forward**); **0 new untracked files in the 24-hour window**); wiki-side carries **9 uncommitted synthesis-layer edits** (carries forward from the 2026-08-22T18:13Z fetch: `.last-update.json` + `quickstart.md` + `sources/README.md` + `sources/hackernews.md` + `sources/nocodb.md` + `sources/ragflow.md` + `sources/seafile.md` + `sources/web-search.md` + `sources/wikijs.md`); **operational issue carries forward** — the `2507925` 2026-08-15 sources-sync count-data wipe (NocoDB 6614 → 0 records, Wiki.js 83 → 0 unique pages, RAGFlow landing 200 → 000) is now re-applied across **8 sources-syncs** (`997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22); the on-disk source configs are unaffected; data is recoverable from `git show 1737454:sources/nocodb.md` etc. — recorded for traceability on `/sources/git-repo.md` Notable-entity § "2026-08-15 sources-sync bug" and on each affected `/sources/{nocodb,ragflow,seafile,wikijs}.md` page; the synthesis layer (themes / commitments / open-questions / personal-logistics) is independent of the source-page counters and is not affected)
+- **Hacker News evidence index** — `/sources/hackernews.md` (active; last refreshed 2026-08-24 18:00 UTC — source-specific LLM-agent topic set (`organoid`, `CelVivo`, `3Brain`, `Cellink`, `openwiki`, `deepagents`); **fourteenth pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries** (after the 2026-08-08 03:15 + 2026-08-08 18:00 + 2026-08-13 + 2026-08-15 + 2026-08-16 + 2026-08-18 + 2026-08-19 + 2026-08-21 + 2026-08-23 + 2026-08-24 fully-zero pulls + the 2026-08-10 + 2026-08-12 + 2026-08-17 + 2026-08-20 + 2026-08-22 noise-only pulls; **2026-08-23 + 2026-08-24 are two consecutive fully-zero Algolia lanes** bookending the 2026-08-22 noise-only pull — 3 fully-zero lanes in 4 consecutive pulls — confirming the empty-pulls-on-vendor-queries cadence is normal under the 6-query LLM-agent topic set, not an outage, no config-side intervention needed) — vendor-anchored footprint remains null on the HN lane and Tavily (web-search) remains the productive lane; the 2026-08-20 pull (prior-prior-prior) produced the `class-organoid-3d-bioprinting` theme-row Last-seen refresh 2026-08-12 → 2026-08-20 via the recurrence criterion (the `49366519` Nature organoid-time-passage paper 18 pts + `49373812` Science Alert cross-walk — second neural-organoid HN signal in 9 pulls), and that refresh carries forward unchanged into the 2026-08-22 + 2026-08-23 + 2026-08-24 pulls (no organoid / 3D-bioprinting item on any of these pulls); **five named-stack-adjacent items flagged for traceability this pull, none promoting a `/themes.md` row**: **`49398152` "Munder Difflin – Agent harness to run an office of your clones"** (192 pts, 81 cmts top, by `simonpure` 2026-08-21, https://munderdiffl.in/ — agent harness for running an office of agent-clones; **strongest named-stack-adjacent item of the pull by direct agent-harness cross-walk** by virtue of (a) 192-pt + 81-cmt top-feed engagement, the third-highest top-feed engagement on the pull; (b) the **agent-harness "office of your clones" framing** cross-walks to the same agent-as-primary-compute-substrate narrative that Robin's `hermes-agent-v0-19-quicksilver` row documents; (c) **5th entry in the now 5-day self-hosted-agentic-substrate cluster** — DeepSeek Harness `49285244` 422 pts 2026-08-13 → machine0 `49348136` 19 pts 2026-08-18 → OneCLI `49363710` 21 pts 2026-08-19 → self-hosted agentic software factory `49390463` 16 pts 2026-08-21 → **Munder Difflin `49398152` 192 pts 2026-08-22** — 5 independent submissions across 5 consecutive working days all corroborating the same narrative, with Munder Difflin's 192-pt engagement being the second-highest of the cluster (after the 422-pt DeepSeek Harness) and **stronger than machine0 + OneCLI + self-hosted factory combined**; **adjacent-not-promoted** per scope because Munder Difflin is **not on Robin's named daily-driver stack** and the established scope rule keeps non-named-stack agent-harness launches adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself; **the cluster is now an emerging-pattern watchlist candidate** with 5 independent submissions across 5 consecutive working days); **`49399591` "New MCP Roadmap"** (120 pts, 93 cmts top, by `pentagrama` 2026-08-21, https://blog.modelcontextprotocol.io/posts/mcp-roadmap/ — official **MCP roadmap from the canonical MCP blog**; **strongest protocol-substrate signal of the pull** because (a) **MCP is the protocol-layer substrate of every named-stack item on Robin's workflow** — Hermes Agent (the `hermes-agent-v0-19-quicksilver` row cites the 2026-07-28 MCP revision finalization), Codex CLI, OpenWiki, RAGFlow (via MCP connectors), the machine0 + OneCLI self-hosted-agentic-substrate cluster entries; (b) **the MCP roadmap is the first MCP-protocol roadmap entry since the 2026-07-28 MCP revision finalization** documented on the `hermes-agent-v0-19-quicksilver` row; (c) the **120-pt + 93-cmt top-feed engagement** is moderate-to-high; (d) the MCP roadmap directly affects the substrate that `agent-memory-architecture` row's MCP Memory entry (the 2026-08-13 OKF+memory schema refresh) sits on; **adjacent-not-promoted** per scope because MCP is the **substrate protocol, not a named-stack vendor itself** and the established scope rule keeps substrate-protocol roadmap entries adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself; **the MCP substrate is its own substrate-cluster watchlist candidate** — MCP Memory 46 pts 2026-08-13 + machine0 MCP support 2026-08-18 + OneCLI MCP connectors 2026-08-19 + **New MCP Roadmap 120 pts 2026-08-22** — 4 distinct substrate-protocol signals across 4 pulls in 10 days); **`49401549` "Anthropic appears to be A/B testing reduced effort levels in Claude Code"** (29 pts, 17 cmts top, by `matthieu_bl` 2026-08-22, https://twitter.com/argofowl/status/2091150597374537729 — Twitter screenshot of Anthropic's apparent Claude CodLine truncated
+- **Web search evidence index** — `/sources/web-search.md` (active; last successful pull 2026-08-03 18:08 UTC — first pull after the 3-day outage streak; CELLINK × Volumetric new Lumen X + 3Brain Utrecht Summer School + CelVivo ClinoStar 2 / Thailand Lab 2026 surfaced, see source page; 2026-08-08, 2026-08-10, 2026-08-12, 2026-08-15, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, 2026-08-23, and 2026-08-24 update attempts skipped — connector not enabled in runtime registry, `TAVILY_API_KEY` missing; disk config shows `enabled: true` + 6 Chinese queries but the runtime registry disagrees — operational concern, not a wiki-synthesis concern; **twelfth consecutive skip on the same code path** including 2026-08-24)
+- **Git repo evidence index** — `/sources/git-repo.md` (active; last refreshed 2026-08-24 18:05 UTC — covers Robin's Obsidian vault (HEAD `fb017e4f` 2026-08-23/24, **5 new vault commits in the 2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window** — **largest single-day LDR burst since the 2026-07-29 Parate / Lee / Hodge cluster**, breaks the **7-fetch vault-silence streak** that started after the 2026-08-16 Quintard + Guo cluster; commit chain `ad4abcde` Ajongbolo 2025 Cells brain organoid review → `5f02489a` Kim 2025 Nat Commun EGaIn 3D MEA → `40b357b8` Tian 2025 Nat Commun hiPSC epicardium mTOR → `f2118a62` Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid CFD → `fb017e4f` CelVivo App Note 2026 ClinoStar vs orbital shaker; all 5 files single-shot entries at `obsidian-vault/04-Literature/Deep-Reads/` root, non-standard pattern vs the prior per-vendor subfolder discipline; vendor-anchor mapping 3 CelVivo-aligned (App Note + Aiello + Ajongbolo mentions ClinoStar) + 1 3Brain-adjacent 3D-MEA competitor (Kim EGaIn) + 1 cardiac epicardium (Tian mTOR); the 2026-08-23T18:11Z fetch was silent (0 new vault commits for the **7th consecutive fetch** before the burst; the 2026-08-24T18:05Z fetch observed the 5-commit burst that broke the streak); the `Review/zhou2023_computer_vision_microfluidics.md` first-entry-in-new-`Review/`-subfolder is at **13-day carry-forward** (mtime `2026-08-11 16:31:54 +0800`), uncommitted since 2026-08-11, structural signal continues to weaken, **11 fetches silent** on the file; new untracked directory **`deepread_40240593/`** (2026-08-24, 7 subdirectories: `deepread_40016200` + `deepread_40441268` + `deepread_40498018` + `deepread_40593704` + `deepread_41151577` + `deepread_brain_organoid_fluid` + `deepread_shear_stress_cs_os` — the numeric-name pattern `deepread_<PMID>` suggests Robin's LDR pipeline uses these directories as per-paper working folders before final commit to `04-Literature/Deep-Reads/`); the 2 prior untracked directories `celvivo_commercial/1331651_20260630_131201.pdf` + `templates/类器官IRB申请清单.md` v1.0 are now at **8-day carry-forward**) + OpenWiki wiki (HEAD `127c2dc` 2026-08-24, **2 new automated wiki commits since the 2026-08-23T18:11Z fetch**: `9cc5e63` chore: daily update 2026-08-24 (2026-08-24T02:23 +0800 — carried the synthesis-layer edits from the prior HN-1 2026-08-23T18:00Z + web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-23T18:11Z runs) + `127c2dc` chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-24, 2026-08-24T02:30 +0800, the **tenth** consecutive sources-sync re-applying the `2507925` 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + `8f8f767` 2026-08-23 + now `127c2dc` 2026-08-24) + the prior 2026-08-23 fetch's 2 wiki commits (`a9568d8` daily update 2026-08-23 + `8f8f767` sources-sync 2026-08-23, the **ninth** consecutive sources-sync); **25 vault commits since 2026-08-03 fetch** (+5 from the prior 20-commit baseline at the 2026-08-23T18:11Z fetch); wiki-side carries **8 uncommitted synthesis-layer edits** (carries forward from the 2026-08-24T18:05Z fetch: `.last-update.json` + `sources/hackernews.md` + `sources/literature.md` + `sources/nocodb.md` + `sources/ragflow.md` + `sources/seafile.md` + `sources/web-search.md` + `sources/wikijs.md`); **operational issue carries forward** — the `2507925` 2026-08-15 sources-sync count-data wipe (NocoDB 6614 → 0 records, Wiki.js 83 → 0 unique pages, RAGFlow landing 200 → 000) is now re-applied across **10 sources-syncs** (`997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + `8f8f767` 2026-08-23 + `127c2dc` 2026-08-24); the on-disk source configs are unaffected; data is recoverable from `git show 1737454:sources/nocodb.md` etc. — recorded for traceability on `/sources/git-repo.md` Notable-entity § "2026-08-15 sources-sync bug" and on each affected `/sources/{nocodb,ragflow,seafile,wikijs}.md` page; the synthesis layer (themes / commitments / open-questions / personal-logistics) is independent of the source-page counters and is not affected; **the 5-paper LDR burst produced the `class-organoid-3d-bioprinting` theme-row Last-seen refresh 2026-08-20 → 2026-08-24** via the recurrence criterion (3 CelVivo-aligned papers in a single burst is the strongest vendor-anchored pattern since the 2026-07-29 cluster) — see `/themes.md` + `/themes/05-robin-research.md` + `/sources/literature.md` for synthesis)
 - **Project / people / company pages** — only created when durable entities emerge from ingested data
 
-These are intentionally blank until there is real evidence to put in them. Populating them with placeholders would dilute the synthesis layer..esis layer..sis platform 4-commit LDR cycle** (`e542c977` adds file 186 lines → `8699a154` page 946 9 项 SOP 重写版 105+/122- → `aacba7aa` frontmatter title 改中文 1 line → `297f106d` page 934 修齐 5+/4-) — **net-new external evidence** (DOI 10.1038/s41598-026-42990-2, PMID 41857098, mouse liver 3 轨道 Hep-Orgs + Cho-Orgs + HSC + DAPT+Dex P1-P10 长程成熟 + TGFβ 激活体外纤维化模型; Wiki.js Id=1716, NocoDB Id=770); **23 untracked vault files** unchanged from the 2026-08-13 fetch (no new untracked files in the 24-hour window) — including 8 × `02-Clients/Agents/区域-2026-0807.md` distributors-rebuild + `_known_associations.md` association index + **the first entry in the new `Review/` subfolder** (`04-Literature/Deep-Reads/Review/zhou2023_computer_vision_microfluidics.md` — Zhou 2023 computer vision + microfluidics, Robin deep-read in progress, still-uncommitted at the 2026-08-15 fetch) + `__pycache__/...pyc` Python build noise; wiki-side carries **10 uncommitted synthesis-layer edits** accumulated across the prior HN-1 2026-08-13 + HN-1 2026-08-15 + web-search-1 2026-08-15 + this git-repo-1 2026-08-15 runs; **one operational issue surfaced this run** — the `2507925` 2026-08-15 sources-sync committed empty count values for NocoDB / SeaFile / Wiki.js / RAGFlow (NocoDB 6614 → 0 records, Wiki.js 83 → 0 unique pages, RAGFlow landing 200 → 000); the on-disk source configs are unaffected; data is recoverable from `git show 1737454:sources/nocodb.md` etc. — recorded for traceability on `/sources/git-repo.md` Notable-entity § "2026-08-15 sources-sync bug" and on each affected `/sources/{nocodb,ragflow,seafile,wikijs}.md` page; the synthesis layer (themes / commitments / open-questions / personal-logistics) is independent of the source-page counters and is not affected)
-- **Project / people / company pages** — only created when durable entities emerge from ingested data
-
-These are intentionally blank until there is real evidence to put in them. Populating them with placeholders would dilute the synthesis layer..there is real evidence to put in them. Populating them with placeholders would dilute the synthesis layer..
+These are intentionally blank until there is real evidence to put in them. Populating them with placeholders would dilute the synthesis layer.

sources/README.md

@@ -2,8 +2,8 @@
 type: "Reference"
 title: "Sources"
 description: "Per-connector evidence indexes and ingestion coverage for the local knowledge wiki; durable synthesis lives on canonical pages."
-tags: [sources, connectors, evidence, ingestion, 2026-08-23]
-timestamp: 2026-08-23T18:11:00Z
+tags: [sources, connectors, evidence, ingestion, 2026-08-24, 2026-08-23]
+timestamp: 2026-08-24T18:05:00Z
 ---
 
 # Sources
@@ -15,7 +15,7 @@ Per-connector evidence indexes. Each connector gets its own page (for example `g
 | Connector | Page | Status |
 | --- | --- | --- |
 | Hacker News (`hackernews-1`, LLM-agent topics) | [/sources/hackernews.md](/sources/hackernews.md) | active (last 2026-08-22 18:00 UTC — twelfth pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries; the 2026-08-22 pull reverts to the noise-only shape (organoid 5 hits + CelVivo 0 + 3Brain 6 hits + Cellink 3 hits + openwiki 0 + deepagents 0) after the 2026-08-21 fully-zero pull — NOT a third fully-zero Algolia lane in a row; the noise-only Algolia substring hits include **1 thematic organoid duplicate** (`49400587` Nautilus "The Remarkable Lives of 5-Year-Old Brain Organoids" 1 pt top, the 3rd neural-organoid HN signal in 11 pulls after the 2026-07-27 Nature biocomputing `49071596` + 2026-08-12 Wired `49266068` + 2026-08-20 Nature `49366519` + Science Alert `49373812`) + **2 agent-memory-architecture adjacents** (`49394827` OzBrain 72 pts top, submitter text explicitly cites "Obsidian vaults with 7k files synced through a VPS, markdown repos behind their own MCP servers" — exact architectural parallel to Robin's OpenWiki + Obsidian vault stack; `49399942` Knowl 1 pt top, open-source MCP-backed agent memory with memory-pruning pattern — same pattern the `agent-memory-architecture` row anchors on); the strongest named-stack-adjacent items are `49398152` "Munder Difflin – Agent harness to run an office of your clones" 192 pts top (agent harness cross-walks to the same agent-as-primary-compute-substrate narrative Robin's `hermes-agent-v0-19-quicksilver` row documents; **5th entry in the now 5-day self-hosted-agentic-substrate cluster** — DeepSeek Harness 2026-08-13 + machine0 2026-08-18 + OneCLI 2026-08-19 + self-hosted agentic software factory 2026-08-21 + **Munder Difflin 2026-08-22** — 5 independent submissions across 5 consecutive working days, with Munder Difflin's 192-pt engagement being the second-highest of the cluster after the 422-pt DeepSeek Harness; cluster is now an emerging-pattern watchlist candidate) + `49399591` "New MCP Roadmap" 120 pts top (official MCP roadmap from the canonical MCP blog at blog.modelcontextprotocol.io — **first MCP-protocol roadmap entry since the 2026-07-28 MCP revision finalization** documented on the `hermes-agent-v0-19-quicksilver` row; **MCP substrate is its own substrate-cluster watchlist candidate** — MCP Memory 2026-08-13 + machine0 MCP support 2026-08-18 + OneCLI MCP connectors 2026-08-19 + **New MCP Roadmap 2026-08-22** — 4 distinct substrate-protocol signals across 4 pulls in 10 days; MCP is the protocol-layer substrate of every named-stack item on Robin's workflow — Hermes Agent / Codex CLI / OpenWiki / RAGFlow / machine0 / OneCLI all depend on MCP), with five additional adjacents flagged for traceability: `49394827` OzBrain 72 pts top (3Brain substring-noise duplicate; direct Obsidian-vault + MCP architectural parallel to Robin's OpenWiki stack; adjacent-not-promoted per scope), `49399942` Knowl 1 pt top (Cellink substring-noise duplicate; open-source MCP-backed agent memory with memory-pruning pattern; adjacent-not-promoted per scope), `49400587` Nautilus 5-Year-Old Brain Organoids 1 pt top (organoid/3Brain substring-noise duplicate; 3rd neural-organoid HN signal in 11 pulls; flagged-for-traceability rather than promoted because 1-pt engagement is below the 5-pt 2026-08-12 Wired + 18-pt 2026-08-20 Nature engagement thresholds that drove prior `class-organoid-3d-bioprinting` Last-seen refreshes), `49401549` Anthropic Claude Code A/B testing reduced effort levels 29 pts top (4th Claude Code traceability entry in 14 days; adjacent-not-promoted per scope — Claude Code is not on Robin's named stack), `49394496` "Three important steps in my maturation process" 199 pts best (Thomas Dullien personal-blog essay; adjacent-not-promoted per scope — off-scope per scope discipline), `49397947` "Embedded AI" 29 pts top (No Starch Press book publication; adjacent-not-promoted per scope — book publication, not a vendor stack change)) |
-| Git repo (`git-repo-1`, Obsidian vault + OpenWiki wiki) | [/sources/git-repo.md](/sources/git-repo.md) | active (last 2026-08-23 18:11 UTC — 0 new vault commits since 2026-08-22T18:13Z fetch (vault silent for the 7th consecutive fetch, **7 calendar days stale** since the 2026-08-16 Quintard + Guo cluster); 2 new automated wiki commits since 2026-08-22T18:13Z fetch (`a9568d8` daily update 2026-08-23 + `8f8f767` sources-sync 2026-08-23, the ninth consecutive sources-sync re-applying the 2026-08-15 count-data wipe pattern)) |
+| Git repo (`git-repo-1`, Obsidian vault + OpenWiki wiki) | [/sources/git-repo.md](/sources/git-repo.md) | active (last 2026-08-24 18:05 UTC — **5 new vault commits** in the 2026-08-23T18:11Z → 2026-08-24T18:05Z window (the largest single-day LDR burst since 2026-07-29: CelVivo App Note 2026 + Aiello & Bagni 2025 biorxiv Rotating Chamber + Tian 2025 Nat Commun epicardium mTOR + Kim 2025 Nat Commun EGaIn 3D MEA + Ajongbolo 2025 Cells brain organoid review — all 5 at `04-Literature/Deep-Reads/` root, non-standard pattern); vault HEAD bumped `a4fa5a32` → `fb017e4f`; 2 new automated wiki commits since 2026-08-23T18:11Z fetch (`9cc5e63` daily update 2026-08-24 + `127c2dc` sources-sync 2026-08-24, the **tenth** consecutive sources-sync re-applying the 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + `8f8f767` 2026-08-23 + now `127c2dc` 2026-08-24); 1 new untracked directory `deepread_40240593/` (7 subdirs, LDR pipeline intermediate-artifact workspace)) |
 | Web Search (`web-search-1`, Tavily) | [/sources/web-search.md](/sources/web-search.md) | active (latest pull 2026-08-03; 2026-08-08, 2026-08-10, 2026-08-12, 2026-08-15, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, and 2026-08-23 update attempts skipped — connector not enabled, `TAVILY_API_KEY` missing) |
 
 Additional connectors appear here automatically once they are authenticated and ingested at least once.
@@ -30,7 +30,7 @@ Additional connectors appear here automatically once they are authenticated and
 | X / Twitter | not configured | `OPENWIKI_X_ACCESS_TOKEN` via `openwiki auth x` | Streams: `home_timeline`, `user_posts`, `mentions`, `bookmarks`, `list_posts`. |
 | Web Search | **active** (`web-search-1`) | `TAVILY_API_KEY` (currently empty) | Tavily via LangChain; reads configured queries. Latest successful pull 2026-08-03 18:08 UTC; the 2026-08-08, 2026-08-10, 2026-08-12, 2026-08-15, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, and 2026-08-23 update attempts were skipped because the connector was not enabled in the runtime registry (eleventh consecutive skip on the same code path; nightly searxng-fallback slot for 2026-08-22 is also empty on disk). Evidence at [/sources/web-search.md](/sources/web-search.md). |
 | Hacker News | **active** (`hackernews-1`) | _(public, no auth)_ | Public feeds (`top`, `new`, `best`) + Algolia HN search queries. Source-specific query set: `organoid`, `CelVivo`, `3Brain`, `Cellink`, `openwiki`, `deepagents` (LLM-agent topics per the source-specific instruction "Track organoid / CelVivo / 3Brain / Cellink / openwiki / AI agent stories"). Last successful pull 2026-08-22 18:00 UTC — **twelfth pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries** (after the 2026-08-08 03:15 + 2026-08-08 18:00 + 2026-08-15 + 2026-08-16 + 2026-08-18 + 2026-08-19 + 2026-08-21 fully-zero pulls + the 2026-08-10 + 2026-08-12 + 2026-08-17 + 2026-08-20 + 2026-08-22 noise-only pulls where vendor queries returned substring-fragment noise; the **2026-08-22 pull reverts to the noise-only shape after the 2026-08-21 fully-zero pull** — substring-noise on organoid/3Brain/Cellink (5+6+3=14 hits) + clean zero on CelVivo/openwiki/deepagents — and is **NOT** the third fully-zero Algolia lane in a row; empty-pulls-on-vendor-queries is normal cadence, not an outage). The two strongest named-stack-adjacent items of the pull are `49398152` "Munder Difflin – Agent harness to run an office of your clones" 192 pts top (agent harness cross-walks to the same agent-as-primary-compute-substrate narrative Robin's `hermes-agent-v0-19-quicksilver` row documents; **5th entry in the now 5-day self-hosted-agentic-substrate cluster** — DeepSeek Harness 2026-08-13 + machine0 2026-08-18 + OneCLI 2026-08-19 + self-hosted agentic software factory 2026-08-21 + **Munder Difflin 2026-08-22** — 5 independent submissions across 5 consecutive working days, with Munder Difflin's 192-pt engagement being the second-highest of the cluster after the 422-pt DeepSeek Harness; cluster is now an emerging-pattern watchlist candidate) + `49399591` "New MCP Roadmap" 120 pts top (official MCP roadmap from the canonical MCP blog at blog.modelcontextprotocol.io; **first MCP-protocol roadmap entry since the 2026-07-28 MCP revision finalization**; **MCP substrate is its own substrate-cluster watchlist candidate** — MCP Memory 2026-08-13 + machine0 MCP support 2026-08-18 + OneCLI MCP connectors 2026-08-19 + **New MCP Roadmap 2026-08-22** — 4 distinct substrate-protocol signals across 4 pulls in 10 days); five additional adjacents flagged for traceability: `49394827` OzBrain 72 pts top (3Brain substring-noise duplicate; direct Obsidian-vault + MCP architectural parallel to Robin's OpenWiki stack; adjacent-not-promoted per scope), `49399942` Knowl 1 pt top (Cellink substring-noise duplicate; open-source MCP-backed agent memory with memory-pruning pattern; adjacent-not-promoted per scope), `49400587` Nautilus 5-Year-Old Brain Organoids 1 pt top (organoid/3Brain substring-noise duplicate; 3rd neural-organoid HN signal in 11 pulls; flagged-for-traceability rather than promoted because 1-pt engagement is below the 5-pt Wired + 18-pt Nature engagement thresholds that drove prior `class-organoid-3d-bioprinting` Last-seen refreshes), `49401549` Anthropic Claude Code A/B testing reduced effort levels 29 pts top (4th Claude Code traceability entry in 14 days; adjacent-not-promoted per scope — Claude Code is not on Robin's named stack), `49394496` "Three important steps in my maturation process" 199 pts best (Thomas Dullien personal-blog essay; adjacent-not-promoted per scope — off-scope per scope discipline), `49397947` "Embedded AI" 29 pts top (No Starch Press book publication; adjacent-not-promoted per scope — book publication, not a vendor stack change). The 2026-08-20 pull (prior-prior) produced the `class-organoid-3d-bioprinting` theme-row Last-seen refresh 2026-08-12 → 2026-08-20 via the recurrence criterion (the `49366519` Nature organoid-time-passage paper 18 pts + `49373812` Science Alert cross-walk — second neural-organoid HN signal in 9 pulls); that refresh carries forward unchanged into the 2026-08-22 pull because the `49400587` Nautilus 5-Year-Old Brain Organoids 1-pt substring-noise duplicate qualifies for the recurrence criterion but 1-pt engagement is below the 5-pt Wired + 18-pt Nature thresholds. All eight 2026-08-22 adjacents stay adjacent-not-promoted per scope discipline. Evidence at [/sources/hackernews.md](/sources/hackernews.md). |
-| Git repo | **active** (`git-repo-1`) | local repo path | Local Obsidian vault at `/home/ldw/workspace` + OpenWiki wiki at `/home/ldw/.openwiki/wiki` (two configured repos per `~/.openwiki/connectors/git-repo/config.json`). Writes compact manifest; repo itself is source of truth. Evidence at [/sources/git-repo.md](/sources/git-repo.md). Last updated 2026-08-23 18:11 UTC — **0 new vault commits in the 24-hour window** (vault silent for the 7th consecutive fetch; the 2026-08-16 Quintard 2024 + Guo 2026 cluster remains the most recent vault activity, **7 calendar days stale**); 2 new automated wiki commits since the 2026-08-22 fetch (`a9568d8` daily update 2026-08-23 + `8f8f767` sources-sync 2026-08-23, the **ninth** consecutive sources-sync re-applying the 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + now `8f8f767` 2026-08-23). |
+| Git repo | **active** (`git-repo-1`) | local repo path | Local Obsidian vault at `/home/ldw/workspace` + OpenWiki wiki at `/home/ldw/.openwiki/wiki` (two configured repos per `~/.openwiki/connectors/git-repo/config.json`). Writes compact manifest; repo itself is source of truth. Evidence at [/sources/git-repo.md](/sources/git-repo.md). Last updated 2026-08-24 18:05 UTC — **5 new vault commits in the 24-hour window** (the largest single-day LDR burst since 2026-07-29: CelVivo App Note 2026 + Aiello & Bagni 2025 biorxiv Rotating Chamber + Tian 2025 Nat Commun epicardium mTOR + Kim 2025 Nat Commun EGaIn 3D MEA + Ajongbolo 2025 Cells brain organoid review — all 5 at `04-Literature/Deep-Reads/` root, non-standard pattern vs the prior per-vendor subfolder discipline); vault HEAD bumped `a4fa5a32` → `fb017e4f`; 2 new automated wiki commits since the 2026-08-23 fetch (`9cc5e63` daily update 2026-08-24 + `127c2dc` sources-sync 2026-08-24, the **tenth** consecutive sources-sync re-applying the 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + `8f8f767` 2026-08-23 + now `127c2dc` 2026-08-24); 1 new untracked directory `deepread_40240593/` (LDR pipeline intermediate-artifact workspace with 7 subdirs: 5 PMID-named `deepread_<PMID>/` + 2 slug-named `deepread_brain_organoid_fluid/` + `deepread_shear_stress_cs_os/` that exactly map to the Aiello + CelVivo App Note papers). |
 
 ## How new source pages get created
 

sources/git-repo.md

@@ -1,10 +1,10 @@
 ---
 type: "Reference"
 title: "Git repo — evidence index (Obsidian vault, Robin)"
-description: "Compact manifest of Robin's Obsidian vault + the local OpenWiki wiki, ingested through the local git-repo connector. Two configured repos: obsidian-vault at /home/ldw/workspace (main branch, HEAD a4fa5a32, 2026-08-16, **0 new vault commits in the 2026-08-22T18:13Z → 2026-08-23T18:11Z 24-hour window — vault is silent for the 7th consecutive fetch after the 2026-08-16 Quintard + Guo cluster, 7 calendar days stale**) and openwiki-wiki at /home/ldw/.openwiki/wiki (main branch, HEAD a9568d8, 2026-08-23, **2 new automated wiki commits in the 2026-08-22T18:13Z → 2026-08-23T18:11Z window: `8f8f767` sources-sync 2026-08-23 + `a9568d8` daily update 2026-08-23 — the ninth consecutive sources-sync re-applying the 2026-08-15 count-data wipe pattern**). This page is a navigation index — content ingestion (paper deep reads, sales scripts, customer files) happens via the Hermes skill and RAGFlow, not here."
+description: "Compact manifest of Robin's Obsidian vault + the local OpenWiki wiki, ingested through the local git-repo connector. Two configured repos: obsidian-vault at /home/ldw/workspace (main branch, HEAD fb017e4f, 2026-08-23/24, **5 new vault commits in the 2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window — the largest single-day LDR burst since the 2026-07-29 Parate / Lee / Hodge cluster: `ad4abcde` Ajongbolo 2025 Cells brain organoid review + `5f02489a` Kim 2025 Nat Commun EGaIn 3D MEA + `40b357b8` Tian 2025 Nat Commun hiPSC epicardium mTOR + `f2118a62` Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid CFD + `fb017e4f` CelVivo App Note 2026 ClinoStar vs orbital shaker — 3 CelVivo-aligned papers (App Note + Aiello + Ajongbolo mentions ClinoStar) + 1 3Brain-adjacent 3D-MEA competitor paper (Kim EGaIn) + 1 cardiac epicardium paper (Tian)**) and openwiki-wiki at /home/ldw/.openwiki/wiki (main branch, HEAD 127c2dc, 2026-08-24, **2 new automated wiki commits in the 2026-08-23T18:11Z → 2026-08-24T18:05Z window: `9cc5e63` daily update 2026-08-24 + `127c2dc` sources-sync 2026-08-24 — the tenth consecutive sources-sync re-applying the 2026-08-15 count-data wipe pattern**). This page is a navigation index — content ingestion (paper deep reads, sales scripts, customer files) happens via the Hermes skill and RAGFlow, not here."
 resource: "~/.openwiki/connectors/git-repo/config.json"
-tags: [git-repo, evidence, vault, openwiki-wiki, source, 2026-08-23, 2026-08-22, 2026-08-21, 2026-08-20, 2026-08-19, 2026-08-18, 2026-08-17, 2026-08-15, 2026-08-13]
-timestamp: 2026-08-23T18:11:00Z
+tags: [git-repo, evidence, vault, openwiki-wiki, source, 2026-08-24, 2026-08-23, 2026-08-22, 2026-08-21, 2026-08-20, 2026-08-19, 2026-08-18, 2026-08-17, 2026-08-15, 2026-08-13, celvivo-app-note-2026, aiello-bagni-rc-cfd, tian-epicardium-mtor, kim-egain-3d-mea, ajongbolo-brain-organoid-review]
+timestamp: 2026-08-24T18:05:00Z
 ---
 
 # Git repo — evidence index (Obsidian vault + OpenWiki wiki)
@@ -22,112 +22,120 @@ help locate things in the vault, not summarize their content.
   `~/.openwiki/connectors/git-repo/config.json`)
 - **Configured repos:**
   - `obsidian-vault` → `/home/ldw/workspace` (Robin Obsidian vault, on
-    `main`, HEAD `a4fa5a323d6dcabeb9eccad49e636ee6ba5f87a6` as of
-    2026-08-16 +0800 — 7 new vault commits in the
-    2026-08-15T18:07Z → 2026-08-17T18:09Z 48-hour window: the 2026-08-16
-    **Quintard 2024 Nat Commun 4-commit LDR cycle** (`0fc8db24` adds
-    file 189 lines → `290e9635` -v2 修图床/双 H1/落款 → `24c713de`
-    -v3 Wiki.js 自带图床 6 张图 → `4073abf4` -v4 转发语标题/落款加粗/删
-    3Brain) + the 2026-08-16 **Guo 2026 Front Cell Dev Biol 3-commit LDR
-    cycle** (`2e6f5989` adds file 170 lines → `00f824f2` -v2 修图床 →
-    `a4fa5a32` -v3 补 Fig 1) — the first time two new paper LDR cycles
-    land within the same 48-hour window since the 2026-07-28 / 2026-07-29
-    Parate / Lee / Hodge cluster. The 2026-08-12 Liang 2026 4-commit LDR
-    cycle remains the prior baseline cluster).
+    `main`, HEAD `fb017e4fb40f874e54fb2a92eff235abe1c487cb` as of
+    2026-08-23/24 +0800 — **5 new vault commits** in the
+    2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window: the
+    **2026-08-23/24 5-paper LDR burst** that broke the 7-day vault
+    silence — `ad4abcde` Ajongbolo 2025 Cells brain organoid review +
+    `5f02489a` Kim 2025 Nat Commun EGaIn 3D MEA + `40b357b8` Tian 2025
+    Nat Commun hiPSC epicardium mTOR + `f2118a62` Aiello & Bagni 2025
+    biorxiv Rotating Chamber brain organoid CFD + `fb017e4f` CelVivo
+    App Note 2026 ClinoStar vs orbital shaker head-to-head. 3 CelVivo-
+    aligned papers (App Note + Aiello + Ajongbolo mentions ClinoStar
+    reagent supply chain) + 1 3Brain-adjacent 3D-MEA competitor paper
+    (Kim EGaIn) + 1 cardiac epicardium paper (Tian); each commit is a
+    single-shot entry (no v2/v3 SOP rewrite cycles this cluster — the
+    page-934 / page-946 alignment work from the 2026-08-12 Liang 2026
+    cluster is no longer in flight). **Largest single-day LDR burst
+    since the 2026-07-29 Parate / Lee / Hodge cluster** — breaks the
+    7-day vault silence streak that started after the 2026-08-16
+    Quintard + Guo cluster).
   - `openwiki-wiki` → `/home/ldw/.openwiki/wiki` (the OpenWiki knowledge
-    base itself, on `main`, HEAD `dcb53a2873d37b6396de682d658433c2ea85b376`
-    as of 2026-08-19T +0800 — 8 new wiki-side commits across the
-    2026-08-15T18:07Z → 2026-08-19T18:08Z window: `151d22b` daily
-    update 2026-08-16 + `997b648` sources-sync 2026-08-16 + `5c70881`
-    daily update 2026-08-17 + `be49285` sources-sync 2026-08-17 +
-    `8127797` daily update 2026-08-18 + `1fc73ae` sources-sync
-    2026-08-18 + `7e904ec` daily update 2026-08-19 + `dcb53a2`
-    sources-sync 2026-08-19; the `5c70881` daily update also created
-    the new `sources/openwiki-version-drift.md` auto-generated page;
-    the 4 sources-syncs (`997b648` + `be49285` + `1fc73ae` + `dcb53a2`)
-    all re-applied the `2507925` 2026-08-15 count-data wipe pattern).
+    base itself, on `main`, HEAD `127c2dc60fb8398d02485313df25994e7e18c69f`
+    as of 2026-08-24T +0800 — 2 new wiki-side commits in the
+    2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window: `9cc5e63`
+    chore: daily update 2026-08-24 + `127c2dc` chore(sources): refresh
+    NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-24) —
+    the **tenth** consecutive sources-sync re-applying the 2026-08-15
+    count-data wipe pattern).
 - **Auth:** local paths only — no remote, no credentials.
-- **Latest fetch:** `2026-08-23T18:11:00Z` (raw manifest
-  `~/.openwiki/connectors/git-repo/raw/2026-08-23T18-11-34-883Z/manifest.json`).
+- **Latest fetch:** `2026-08-24T18:05:00Z` (raw manifest
+  `~/.openwiki/connectors/git-repo/raw/2026-08-24T18-05-31-919Z/manifest.json`).
 - **Source config:** `~/.openwiki/connectors/git-repo/config.json`
 - **State:** `~/.openwiki/connectors/git-repo/state.json` records
-  `latestIds` per repo; the 2026-08-23T18:11Z fetch observed vault HEAD
-  unchanged at `a4fa5a32` (2026-08-16 — same as the 2026-08-17T18:09Z +
+  `latestIds` per repo; the 2026-08-24T18:05Z fetch observed vault HEAD
+  bumped from `a4fa5a32` (2026-08-16 — same as the 2026-08-17T18:09Z +
   2026-08-18T18:10Z + 2026-08-19T18:08Z + 2026-08-20T18:09Z +
-  2026-08-21T18:09Z + 2026-08-22T18:13Z + 2026-08-23T18:11Z fetches;
-  **0 new vault commits in the 24-hour window — vault is silent for
-  the 7th consecutive fetch** after the 2026-08-16 Quintard + Guo
-  cluster, **7 calendar days stale**), and observed `openwiki-wiki`
-  bumped from `43d4548` (2026-08-22T18:13Z fetch) to `a9568d8`
-  (2 new automated wiki commits in the 24-hour window: `8f8f767`
-  sources-sync 2026-08-23 + `a9568d8` daily update 2026-08-23 — the
-  **ninth** consecutive sources-sync re-applying the 2026-08-15
+  2026-08-21T18:09Z + 2026-08-22T18:13Z + 2026-08-23T18:11Z fetches
+  through 7 consecutive silent windows, **7 calendar days stale**) to
+  `fb017e4f` (2026-08-23/24 — **5 new vault commits** in the 24-hour
+  window, breaking the 7-day silence streak), and observed
+  `openwiki-wiki` bumped from `a9568d8` (2026-08-23T18:11Z fetch) to
+  `127c2dc` (2 new automated wiki commits in the 24-hour window:
+  `9cc5e63` daily update 2026-08-24 + `127c2dc` sources-sync 2026-08-24
+  — the **tenth** consecutive sources-sync re-applying the 2026-08-15
   count-data wipe pattern after `997b648` 2026-08-16 + `be49285`
   2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339`
-  2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + now
-  `8f8f767` 2026-08-23).
+  2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + `8f8f767`
+  2026-08-23 + now `127c2dc` 2026-08-24).
 
-## obsidian-vault — working-tree status (2026-08-23)
+## obsidian-vault — working-tree status (2026-08-24)
 
 - **Branch:** `main`
-- **HEAD:** `a4fa5a323d6dcabeb9eccad49e636ee6ba5f87a6` (vault HEAD
-  **unchanged** at `a4fa5a32` across the 2026-08-23T18:11Z fetch —
-  **0 new commits** in the 2026-08-22T18:13Z → 2026-08-23T18:11Z
-  24-hour window; the vault is silent for the **7th consecutive
-  fetch** after the 2026-08-17T18:09Z + 2026-08-18T18:10Z +
-  2026-08-19T18:08Z + 2026-08-20T18:09Z + 2026-08-21T18:09Z +
-  2026-08-22T18:13Z silent
-  windows. The 2026-08-16 Quintard 2024 Nat Commun 4-commit LDR cycle
-  + Guo 2026 Front Cell Dev Biol 3-commit LDR cycle remain the most
-  recent committed vault activity (vault HEAD last bumped on 2026-08-16,
-  **7 calendar days before this fetch** — same state as the
+- **HEAD:** `fb017e4fb40f874e54fb2a92eff235abe1c487cb` (vault HEAD
+  **bumped** from `a4fa5a32` (2026-08-16) → `fb017e4f` (2026-08-23/24)
+  across the 2026-08-24T18:05Z fetch — **5 new commits** in the
+  2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window; the vault
+  breaks the **7-fetch silent streak** that started after the
   2026-08-17T18:09Z + 2026-08-18T18:10Z + 2026-08-19T18:08Z +
-  2026-08-20T18:09Z + 2026-08-21T18:09Z + 2026-08-22T18:13Z fetches).
-- **mtime activity in window:** **0 new mtime changes** in the
-  2026-08-22T18:13Z → 2026-08-23T18:11Z 24-hour window. The 2
+  2026-08-20T18:09Z + 2026-08-21T18:09Z + 2026-08-22T18:13Z +
+  2026-08-23T18:11Z silent windows. The 2026-08-23/24 cluster is
+  **the largest single-day LDR burst since the 2026-07-29 Parate /
+  Lee / Hodge cluster** — 5 paper deep-reads added in <24h, all
+  single-shot entry (no v2/v3 SOP rewrite cycles this cluster, vs the
+  3-stage SOP cycle on the 2026-08-12 Liang 2026 cluster and the
+  4-commit / 3-commit LDR cycles on the 2026-08-16 Quintard + Guo
+  cluster). The 2026-08-16 Quintard 2024 Nat Commun + Guo 2026 Front
+  Cell Dev Biol cluster has been replaced as the most recent committed
+  vault activity after a **7-day stale period** (now stale 0 days).
+- **mtime activity in window:** **5 new committed files** in the
+  2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window — all 5 new
+  paper LDR files at `obsidian-vault/04-Literature/Deep-Reads/` root
+  (no subfolder) per the manifest paths `Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid.md`
+  + `Ajongbolo_2025_Cells_Brain_Organoids_Review.md` +
+  `CelVivo_Shear_Stress_AppNote_2026.md` +
+  `Kim_2025_NatCommun_Liquid_Metal_3D_MEA.md` +
+  `Tian_2025_NatCommun_Epicardium_mTOR.md`. Commit order (mtime
+  ascending): `ad4abcde` 2026-08-24 07:50 Ajongbolo review → `5f02489a`
+  2026-08-24 08:05 Kim LM 3D MEA → `40b357b8` 2026-08-24 09:00 Tian
+  epicardium mTOR → `f2118a62` 2026-08-24 16:23 Aiello RC brain
+  organoid → `fb017e4f` 2026-08-24 16:39 CelVivo App Note 2026 — the
+  burst spans the full 2026-08-24 day from 07:50 to 16:39 +0800. The 2
   untracked dirs from the 2026-08-17T18:09Z fetch are unchanged:
   `celvivo_commercial/` last touched 2026-08-17 15:04 +0800 (= 07:04
-  UTC, **before** the 24h window) + `templates/` last touched
-  2026-08-16 10:27 +0800 (= 02:27 UTC, well before the window). The
-  `Review/zhou2023_computer_vision_microfluidics.md` first-entry
-  file is unchanged from the 2026-08-17T18:09Z fetch (mtime
-  2026-08-11, **12-day carry-forward** in the untracked set at this
-  fetch — was 11-day at the 2026-08-22T18:13Z fetch; the structural
-  signal continues to weaken per the Notable-entity entry on the
-  `Review/` subfolder).
-- **Ahead of origin/main:** 20 commits new since 2026-08-03 fetch (unchanged from the 2026-08-17T18:09Z + 2026-08-18T18:10Z + 2026-08-19T18:08Z + 2026-08-20T18:09Z + 2026-08-21T18:09Z + 2026-08-22T18:13Z + 2026-08-23T18:11Z fetches — vault is silent in the 2026-08-22T18:13Z → 2026-08-23T18:11Z window).
-- **Dirty:** yes — the untracked file set is **unchanged** from the
-  2026-08-17T18:09Z fetch (no new untracked files or directories
-  appeared in the 2026-08-17T18:09Z → 2026-08-18T18:10Z 24-hour
-  window, no new untracked files or directories appeared in the
-  2026-08-18T18:10Z → 2026-08-19T18:08Z 24-hour window, no new
-  untracked files or directories appeared in the 2026-08-19T18:08Z →
-  2026-08-20T18:09Z 24-hour window, no new untracked files or
-  directories appeared in the 2026-08-20T18:09Z → 2026-08-21T18:09Z
-  24-hour window, no new untracked files or directories appeared in
-  the 2026-08-21T18:09Z → 2026-08-22T18:13Z 24-hour window, and no
-  new untracked files or directories appeared in the
-  2026-08-22T18:13Z → 2026-08-23T18:11Z 24-hour window either).
-  The 25+ untracked entries from the
-  2026-08-17T18:09Z fetch carry forward unchanged: 8 ×
-  `02-Clients/Agents/区域-2026-0807.md` distributor-rebuild files
-  (per the 2026-08-07 Hermes hook 4 sweep) + 14 working-folder files
-  (Parfitt 2024 DJ1 LOF midbrain + Liu2026 / Miao2025 v2 /
-  Miao2025 vascularized / Microbioreactor 2026 /
-  `__pycache__/literature_deepread_v6.cpython-312.pyc` /
-  `dify精读进度-20260805.md` / `literature_deepread_v6.py` /
-  `obsidian-vault/04-Literature/Deep-Reads/Review/zhou2023_computer_vision_microfluidics.md`
-  / `obsidian-vault/rustdesk-selfhosted-2026-08-05.md` /
-  `retinal_organoid_deep_read_2026-08-07.md` / `文悦精读流水线.yml` +
-  v2/v3/v4/v5-clean variants) + 2 new untracked working-tree
-  directories from the 2026-08-17T18:09Z fetch (`celvivo_commercial/`
-  + `templates/`). The **`Review/zhou2023_computer_vision_microfluidics.md`
-  first entry in the new `Review/` subfolder** is still **untracked**
-  (Robin has not yet committed it) — **12-day carry-forward** at this
-  fetch (was 11-day at the 2026-08-22T18:13Z fetch); the structural
-  signal continues to weaken per the Notable-entity entry on the
-  `Review/` subfolder (the subfolder has been a single-entry watchlist
-  since 2026-08-13, now **10 fetches silent**).
+  UTC) + `templates/` last touched 2026-08-16 10:27 +0800 (= 02:27
+  UTC). The `Review/zhou2023_computer_vision_microfluidics.md`
+  first-entry file is unchanged from the 2026-08-17T18:09Z fetch
+  (mtime 2026-08-11, **13-day carry-forward** in the untracked set at
+  this fetch — was 12-day at the 2026-08-23T18:11Z fetch; the
+  structural signal continues to weaken per the Notable-entity entry
+  on the `Review/` subfolder). **NEW this fetch**: 1 new untracked
+  working dir `deepread_40240593/` (mtime 2026-08-24 16:31 +0800 = 08:31
+  UTC, within the 24h window) — pipeline scratch workspace
+  containing 7 PMID-specific subdirs (`deepread_40016200` /
+  `deepread_40441268` / `deepread_40498018` / `deepread_40593704` /
+  `deepread_41151577` + 2 named subdirs `deepread_brain_organoid_fluid`
+  [Aiello paper] + `deepread_shear_stress_cs_os` [CelVivo App Note]).
+  The 5 PMIDs are not currently on the vault `main` as deep-read files,
+  suggesting the `deepread_40240593/` is the pipeline's pending-triage
+  workspace for the 2026-08-24 batch.
+- **Ahead of origin/main:** 25 commits new since 2026-08-03 fetch (+5
+  from the prior 20-commit baseline at the 2026-08-23T18:11Z fetch —
+  the 5 new commits land on top of the 20-commit carry-forward).
+- **Dirty:** yes — the untracked file set is **partially changed**:
+  the 8 × `02-Clients/Agents/区域-2026-0807.md` distributor-rebuild
+  files + 14 working-folder files + 2 untracked dirs from the
+  2026-08-17T18:09Z fetch carry forward unchanged, and the
+  `Review/zhou2023_computer_vision_microfluidics.md` first-entry in the
+  new `Review/` subfolder is still **untracked** (Robin has not yet
+  committed it) — **13-day carry-forward** at this fetch (was 12-day
+  at the 2026-08-23T18:11Z fetch); the structural signal continues to
+  weaken per the Notable-entity entry on the `Review/` subfolder (the
+  subfolder has been a single-entry watchlist since 2026-08-13, now
+  **11 fetches silent**). **NEW this fetch**: 1 new untracked working
+  directory `deepread_40240593/` (created 2026-08-24 16:31 +0800 =
+  08:31 UTC, **0-day carry-forward** at this fetch — first observation,
+  pipeline scratch workspace).
 - **Working-tree status (raw manifest, abbreviated):**
 
   | Status | Path |
@@ -174,6 +182,11 @@ help locate things in the vault, not summarize their content.
 
 | Date | Hash | Note |
 | --- | --- | --- |
+| 2026-08-23 | `fb017e4f` | **feat: CelVivo App Note 2026 shear stress ClinoStar vs orbital shaker** (Wiki.js 1747) — adds `obsidian-vault/04-Literature/Deep-Reads/CelVivo_Shear_Stress_AppNote_2026.md` (10,145 bytes, single-shot entry) — **NEW vendor-anchored CelVivo evidence**: Stougaard Borgen Løschenkohl, Sedighi Frandsen (CelVivo App Note 2026) head-to-head ClinoStar® vs orbital shaker, 15-day HepG2/C3A + embedded Caco-2 + brain organoid; F-actin + DAPI staining shows ClinoStar preserves cytoskeleton integrity vs orbital shaker structural decay; ATP + BCA + 3 biological replicates per condition; SP5D 24-well ball-plate 1000 cells/aggregate pre-conditioning; ClinoReactor® vessel (CelVivo 10004-12) in ClinoStar® system (30003) vs ULA 6-well + Steinberg SBS-LVS-100 shaker; low-shear fFSS confirmation |
+| 2026-08-23 | `f2118a62` | **feat: Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid 精读** (Wiki.js 1746) — adds `obsidian-vault/04-Literature/Deep-Reads/Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid.md` (11,731 bytes, single-shot entry) — **NEW CelVivo-aligned evidence**: EMBO Reports 投稿版 (Aiello_Bagni_EMBO R_050325) — COMSOL Multiphysics 6.2 CFD quantification of Rotating Chamber (RC) shear stress field; chamber geometry 78.3 mm × 24.2 mm (outer) / 37.7 mm × 8.8 mm (inner); **shear stress < 20 mPa confirmed** (vs qualitative "low-shear" only previously); 0-300 objects per chamber all stable low-shear across densities 1010/1020/1030; multi-batch + multi-iPSC line brain organoid morphology PCA (CV <15% vs static >30%); scRNA-seq OXPHOS profiling; SOX2/Nestin/PAX6 + DCX + TBR1/SATB2/MAP2 multi-marker IHC; first **quantitative** confirmation of CelVivo RC low-shear claim |
+| 2026-08-23 | `40b357b8` | **feat: Tian 2025 NatCommun 心外膜 mTOR 精读** (Wiki.js 1745) — adds `obsidian-vault/04-Literature/Deep-Reads/Tian_2025_NatCommun_Epicardium_mTOR.md` (15,578 bytes, single-shot entry) — **NEW cardiac organoid evidence**: Tian Y, Lucena-Cacace A, Tani K et al. (2025) Nat Commun DOI 10.1038/s41467-025-60934-8 — Torin1 (dual mTORC1/2 inhibitor, 200 nM) drives hiPSC epicardium maturation from embryonic to adult phenotype; qHSC→aHSC EMT reactivation pathway; bulk RNA-seq 379 DEGs; PCA explains 28.53% PC1 + 36.01% PC2 variance; co-culture hiPSC epicardium + ventricular cardiomyocyte EB → TNNI3 upregulation (paracrine maturation); mTORC1/2 dual inhibition is critical (Rapamycin alone insufficient); YBX3 + MAFF TF candidates via siRNA knockdown; first cardiac epicardium paper since MOC 心肌 maturation track started |
+| 2026-08-23 | `5f02489a` | **feat: Kim 2025 NatCommun 液态金属 3D MEA 精读** (Wiki.js 1744) — adds `obsidian-vault/04-Literature/Deep-Reads/Kim_2025_NatCommun_Liquid_Metal_3D_MEA.md` (13,567 bytes, single-shot entry) — **NEW 3D-MEA / electrophysiology evidence**: Kim E, Jeong E, Hong YM et al. (2025) Nat Commun DOI 10.1038/s41467-024-55752-3 — magnetically reshapable EGaIn (eutectic gallium-indium) liquid-metal 3D MEA pillar array via microfluidic direct-ink-writing; pillar height 25-310 µm linearly controlled by print speed (R²>0.95, n=12, SEM<5%); pillar diameter to 14 µm (single-neuron scale); parylene-C selective sidewall encapsulation (only tip conductive); hPSC cortical organoid 120-day maturation (dual-SMAD inhibition + FGF2/EGF + BDNF/NT3); 6-marker IHC (MAP2/GFAP/VGLUT1/SATB2/CTIP2) showing 6-layer cortical-like structure; Ni magnetic layer for magnetic reshaping; 64-channel MEA + SU spike + network synchronization; **third flexible-electronics / 3D-MEA paper this cycle** after Liu 2026 (NBME shape-conformal porous framework) + Sharma 2026 (60-channel MCS MEA retinal organoid) — direct 3Brain HD-MEA competitive pressure signal |
+| 2026-08-23 | `ad4abcde` | **feat: Ajongbolo 2025 Cells 脑类器官综述精读** (Wiki.js 1743) — adds `obsidian-vault/04-Literature/Deep-Reads/Ajongbolo_2025_Cells_Brain_Organoids_Review.md` (11,367 bytes, single-shot entry) — **NEW brain-organoid review evidence**: Ajongbolo AO, Langhans SA (2025) Cells DOI 10.3390/cells14110842 — comprehensive brain-organoid + assembloid review covering (i) whole-brain vs region-specific vs complex systems (assembloid + OoC + tumoroid + transplant); (ii) 4-tier characterization (morphological / biochemical / functional / omics); (iii) disease modeling across neurodevelopmental (microcephaly / macrocephaly / ASD / Rett / fragile X / Timothy syndrome) + neurodegenerative (AD / PD / HD / ALS) + brain tumor (glioblastoma / medulloblastoma / AT/RT); (iv) drug discovery + HTS pathways; first brain-organoid review since the 2026-07-29 Organoids 2026 disease-modeling review; **mentions ClinoStar / orbital shaker reagent-supply chain** |
 | 2026-08-16 | `a4fa5a32` | **literature: review 类 Guo 2026 -v3 补 Fig 1** — in-place update ID 1724, 3 张图全 |
 | 2026-08-16 | `00f824f2` | **literature: review 类 Guo 2026 -v2 修图床** — in-place update ID 1724, 加 2 张 Wiki.js 自带图 |
 | 2026-08-16 | `2e6f5989` | **literature: 精读 Guo 2026 Front Cell Dev Biol - 类器官伦理审查** (review 类, Wiki ID 1724, NocoDB Id 3298) — **NEW external evidence** (类器官研究 IRB / 伦理审查 7 大强制要点系统综述, 嘉兴市第一医院 + 嘉兴大学医学院视角) |
@@ -337,27 +350,26 @@ indexed on [`/sources/literature.md`](/sources/literature.md). The
 `1614ccf9`) are the 8-step SOP rewrite cycle (v3 → v11) Robin re-ran
 on the same paper.
 
-## openwiki-wiki — working-tree status (2026-08-23)
+## openwiki-wiki — working-tree status (2026-08-24)
 
 - **Branch:** `main`
-- **HEAD:** `a9568d8` (the
-  `chore: daily update 2026-08-23` commit). The 2026-08-23T18:11Z
-  fetch observed wiki HEAD bumped from `43d4548` (2026-08-22T18:13Z
-  fetch) to `a9568d8` — **2 new automated wiki commits** in the
-  2026-08-22T18:13Z → 2026-08-23T18:11Z 24-hour window: (a) `8f8f767`
-  chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts
-  (sources-sync 2026-08-23, 2026-08-23 02:30 +0800); (b) `a9568d8`
-  chore: daily update 2026-08-23 (2026-08-23 02:29 +0800). Both
-  commits are routine auto-orchestration — the `a9568d8` daily update
-  carried the synthesis-layer edits from the prior HN-1
-  2026-08-22T18:00Z + web-search-1 2026-08-22 (skipped) + web-search-1
-  2026-08-23 (skipped) + git-repo-1 2026-08-22T18:13Z runs; the
-  `8f8f767` sources-sync re-applied the count-data wipe pattern on
-  NocoDB / SeaFile / Wiki.js / RAGFlow for the **ninth** consecutive
-  time (after `997b648` 2026-08-16 + `be49285` 2026-08-17 +
-  `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20
-  + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + now `8f8f767`
-  2026-08-23).
+- **HEAD:** `127c2dc` (the
+  `chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-24)` commit). The 2026-08-24T18:05Z
+  fetch observed wiki HEAD bumped from `a9568d8` (2026-08-23T18:11Z
+  fetch) to `127c2dc` — **2 new automated wiki commits** in the
+  2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window: (a) `9cc5e63`
+  chore: daily update 2026-08-24 (2026-08-24 02:23 +0800); (b)
+  `127c2dc` chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts
+  (sources-sync 2026-08-24, 2026-08-24 02:30 +0800). Both commits are
+  routine auto-orchestration — the `9cc5e63` daily update carried the
+  synthesis-layer edits from the prior HN-1 2026-08-23T18:00Z +
+  web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-23T18:11Z
+  runs; the `127c2dc` sources-sync re-applied the count-data wipe
+  pattern on NocoDB / SeaFile / Wiki.js / RAGFlow for the **tenth**
+  consecutive time (after `997b648` 2026-08-16 + `be49285` 2026-08-17
+  + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20
+  + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + `8f8f767` 2026-08-23
+  + now `127c2dc` 2026-08-24).
 - **Working-tree state:** 6 modified files (uncommitted — pending
   next CLI orchestration). The 2026-08-23T18:11Z fetch observed the
   working tree at 6 uncommitted files (down from 9 at the
@@ -449,6 +461,8 @@ on the same paper.
 
 | Date | Hash | Note |
 | --- | --- | --- |
+| 2026-08-24 | `127c2dc` | chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-24) — **count-data wipe re-applied** for the **tenth** consecutive sources-sync (after 2026-08-15 `2507925` + 2026-08-16 `997b648` + 2026-08-17 `be49285` + 2026-08-18 `1fc73ae` + 2026-08-19 `dcb53a2` + 2026-08-20 `bd30339` + 2026-08-21 `f6c2576` + 2026-08-22 `43d4548` + 2026-08-23 `8f8f767`); see Notable-entity § "2026-08-15 sources-sync bug" |
+| 2026-08-24 | `9cc5e63` | chore: daily update 2026-08-24 (synthesis-layer edits accumulated from the prior HN-1 2026-08-23T18:00Z + web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-23T18:11Z runs; committed `sources/git-repo.md` + `sources/hackernews.md` + `sources/index.md` + `sources/openwiki-version-drift.md` + `sources/web-search.md` + `themes/05-robin-research.md` from the prior dirty set, reducing uncommitted-file count from 6 → 8 because the very next synthesis edits from this git-repo-1 2026-08-24T18:05Z run re-touch the same 6 files + add 2 newly dirty files `sources/literature.md` + `sources/nocodb.md` + `sources/ragflow.md` + `sources/seafile.md` + `sources/wikijs.md` per the manifest `changedFiles` list) |
 | 2026-08-23 | `a9568d8` | chore: daily update 2026-08-23 (synthesis-layer edits accumulated from the prior HN-1 2026-08-22T18:00Z + web-search-1 2026-08-22 (skipped) + web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-22T18:13Z runs; committed `sources/git-repo.md` + `sources/index.md` + `sources/nocodb.md` + `sources/ragflow.md` + `sources/seafile.md` + `sources/wikijs.md` + `themes/05-robin-research.md` from the prior dirty set, reducing uncommitted-file count from 9 → 6) |
 | 2026-08-23 | `8f8f767` | chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-23) — **count-data wipe re-applied** for the **ninth** consecutive sources-sync (after 2026-08-15 `2507925` + 2026-08-16 `997b648` + 2026-08-17 `be49285` + 2026-08-18 `1fc73ae` + 2026-08-19 `dcb53a2` + 2026-08-20 `bd30339` + 2026-08-21 `f6c2576` + 2026-08-22 `43d4548`); see Notable-entity § "2026-08-15 sources-sync bug" |
 | 2026-08-22 | `43d4548` | chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-22) — **count-data wipe re-applied** for the **eighth** consecutive sources-sync (after 2026-08-15 `2507925` + 2026-08-16 `997b648` + 2026-08-17 `be49285` + 2026-08-18 `1fc73ae` + 2026-08-19 `dcb53a2` + 2026-08-20 `bd30339` + 2026-08-21 `f6c2576`); see Notable-entity § "2026-08-15 sources-sync bug" |
@@ -533,6 +547,7 @@ pipeline catches up.
 
 | Window (UTC) | Vault | Wiki |
 | --- | --- | --- |
+| 2026-08-23T18:11:00Z → 2026-08-24T18:05:00Z | HEAD `a4fa5a32` → `fb017e4f` (**5 new commits**: `ad4abcde` Ajongbolo 2025 Cells brain organoid review + `5f02489a` Kim 2025 Nat Commun EGaIn 3D MEA + `40b357b8` Tian 2025 Nat Commun hiPSC epicardium mTOR + `f2118a62` Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid CFD + `fb017e4f` CelVivo App Note 2026 ClinoStar vs orbital shaker head-to-head — **largest single-day LDR burst since the 2026-07-29 Parate / Lee / Hodge cluster**, breaks the **7-fetch vault silence streak** that started after the 2026-08-16 Quintard + Guo cluster; all 5 are single-shot entries at `obsidian-vault/04-Literature/Deep-Reads/` root (no v2/v3 SOP rewrite cycles this cluster); commit timestamps 07:50 → 16:39 +0800 across the 2026-08-24 day) | HEAD `a9568d8` → `127c2dc` (**2 new automated commits**: `9cc5e63` chore: daily update 2026-08-24 + `127c2dc` chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-24, the **tenth** consecutive sources-sync re-applying the `2507925` 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + `bd30339` 2026-08-20 + `f6c2576` 2026-08-21 + `43d4548` 2026-08-22 + `8f8f767` 2026-08-23 + now `127c2dc` 2026-08-24) + the prior `a9568d8` 2026-08-23 daily update was committed in this fetch's prior daily update cycle before the 5-paper burst landed; `9cc5e63` committed `sources/git-repo.md` + `sources/hackernews.md` + `sources/index.md` + `sources/openwiki-version-drift.md` + `sources/web-search.md` + `themes/05-robin-research.md` from the prior dirty set; the manifest `changedFiles` shows 8 dirty files this fetch — `M .last-update.json` + `M sources/hackernews.md` + `M sources/literature.md` + `M sources/nocodb.md` + `M sources/ragflow.md` + `M sources/seafile.md` + `M sources/web-search.md` + `M sources/wikijs.md`) |
 | 2026-08-15T18:07:49Z → 2026-08-17T18:09:32Z | HEAD `297f106d` → `a4fa5a32` (**7 new commits**: `0fc8db24` + `290e9635` + `24c713de` + `4073abf4` Quintard 2024 Nat Commun microfluidic vascularized organoid chip 4-commit LDR cycle + `2e6f5989` + `00f824f2` + `a4fa5a32` Guo 2026 Front Cell Dev Biol organoid ethics systematic review 3-commit LDR cycle — **first time since the 2026-07-28 / 2026-07-29 Parate / Lee / Hodge cluster that two new paper deep-reads landed on `main` in the same 48-hour window**) | HEAD `2507925` → `be49285` (**4 new commits**: `151d22b` daily update 2026-08-16 + `997b648` sources-sync 2026-08-16 + `5c70881` daily update 2026-08-17 + `be49285` sources-sync 2026-08-17) |
 | 2026-08-17T18:09:32Z → 2026-08-18T18:10:12Z | HEAD **unchanged** at `a4fa5a32` (**0 new commits**, **0 new mtime changes** — vault is silent in this 24-hour window; the 2026-08-16 Quintard 2024 + Guo 2026 cluster remains the most recent vault activity) | HEAD `be49285` → `1fc73ae` (**2 new automated commits**: `8127797` chore: daily update 2026-08-18 + `1fc73ae` chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-18, the **fourth** consecutive sources-sync re-applying the `2507925` 2026-08-15 count-data wipe pattern)) |
 | 2026-08-19T18:08:24Z → 2026-08-20T18:09:15Z | HEAD **unchanged** at `a4fa5a32` (**0 new commits**, **0 new mtime changes** — vault is silent in this 24-hour window for the **4th consecutive fetch**; the 2026-08-16 Quintard 2024 + Guo 2026 cluster remains the most recent vault activity, **4 calendar days stale**) | HEAD `dcb53a2` → `bd30339` (**2 new automated commits**: `a44f284` chore: daily update 2026-08-20 + `bd30339` chore(sources): refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync 2026-08-20, the **sixth** consecutive sources-sync re-applying the `2507925` 2026-08-15 count-data wipe pattern after `997b648` 2026-08-16 + `be49285` 2026-08-17 + `1fc73ae` 2026-08-18 + `dcb53a2` 2026-08-19 + now `bd30339` 2026-08-20)) |
@@ -577,6 +592,7 @@ Markdown file counts per deep-read subfolder:
 | Subfolder | .md count |
 | --- | --- |
 | `Organoid/` | 661 (+2 from 2026-08-16 — Quintard 2024 Nat Commun microfluidic vascularized organoid chip + Guo 2026 Front Cell Dev Biol organoid ethics systematic review) |
+| `04-Literature/Deep-Reads/` root | +5 new files on 2026-08-23 single-shot LDR burst — CelVivo_Shear_Stress_AppNote_2026 + Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid + Tian_2025_NatCommun_Epicardium_mTOR + Kim_2025_NatCommun_Liquid_Metal_3D_MEA + Ajongbolo_2025_Cells_Brain_Organoids_Review (all in vault root, not in a per-vendor subfolder — this is a **non-standard pattern** vs the prior per-vendor subfolder discipline, see Notable-entity § "2026-08-23 root-level LDR burst") |
 | `Celvivo/` | 142 |
 | `3Brain/` | 136 |
 | `Cellink/` | 129 |
@@ -594,14 +610,15 @@ Markdown file counts per deep-read subfolder:
 | `Retinal/` | 1 |
 | `Microbioreactor/` | 1 (new as of 2026-08-07 — Rosero 2026 Front Bioeng Biotechnol) |
 | `Cells/` | 1 (new as of 2026-08-08 — Fernandez Vallone 2026 Cells THSDC) |
-| `Review/` | 1 (new as of 2026-08-13 — Zhou 2023 computer vision + microfluidics, **untracked**, **10-day carry-forward** at 2026-08-21 fetch, **8 fetches silent** — structural signal continuing to weaken; **11-day carry-forward** at 2026-08-22 fetch, **9 fetches silent**; **12-day carry-forward** at 2026-08-23 fetch, **10 fetches silent**) |
-| `templates/` | 1 (new as of 2026-08-17 — `类器官IRB申请清单.md`, derived from the Guo 2026 ethics deep-read; Robin's first customer-facing template produced from a single deep-read, **untracked**, **5-day carry-forward** at 2026-08-21 fetch (was 4-day at 2026-08-20); **6-day carry-forward** at 2026-08-22 fetch; **7-day carry-forward** at 2026-08-23 fetch |
-| `celvivo_commercial/` | 1 (new as of 2026-08-17 — `1331651_20260630_131201.pdf`, the 6-30 quote PDF migrated into a new folder, **untracked**, **5-day carry-forward** at 2026-08-21 fetch (was 4-day at 2026-08-20); **6-day carry-forward** at 2026-08-22 fetch; **7-day carry-forward** at 2026-08-23 fetch |
+| `Review/` | 1 (new as of 2026-08-13 — Zhou 2023 computer vision + microfluidics, **untracked**, **10-day carry-forward** at 2026-08-21 fetch, **8 fetches silent** — structural signal continuing to weaken; **11-day carry-forward** at 2026-08-22 fetch, **9 fetches silent**; **12-day carry-forward** at 2026-08-23 fetch, **10 fetches silent**; **13-day carry-forward** at 2026-08-24 fetch, **11 fetches silent** — structural signal continues to weaken) |
+| `templates/` | 1 (new as of 2026-08-17 — `类器官IRB申请清单.md`, derived from the Guo 2026 ethics deep-read; Robin's first customer-facing template produced from a single deep-read, **untracked**, **5-day carry-forward** at 2026-08-21 fetch (was 4-day at 2026-08-20); **6-day carry-forward** at 2026-08-22 fetch; **7-day carry-forward** at 2026-08-23 fetch; **8-day carry-forward** at 2026-08-24 fetch) |
+| `celvivo_commercial/` | 1 (new as of 2026-08-17 — `1331651_20260630_131201.pdf`, the 6-30 quote PDF migrated into a new folder, **untracked**, **5-day carry-forward** at 2026-08-21 fetch (was 4-day at 2026-08-20); **6-day carry-forward** at 2026-08-22 fetch; **7-day carry-forward** at 2026-08-23 fetch; **8-day carry-forward** at 2026-08-24 fetch) |
+| `04-Literature/Deep-Reads/` root (5 new files) | 5 (new as of 2026-08-23/24 — single-shot LDR burst: `CelVivo_Shear_Stress_AppNote_2026.md` + `Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid.md` + `Tian_2025_NatCommun_Epicardium_mTOR.md` + `Kim_2025_NatCommun_Liquid_Metal_3D_MEA.md` + `Ajongbolo_2025_Cells_Brain_Organoids_Review.md` — all landed at the root of `04-Literature/Deep-Reads/` not in a per-vendor subfolder; **non-standard pattern** vs the prior per-vendor subfolder discipline, see Notable-entity § "2026-08-23 root-level LDR burst") |
 
-Total ≈ 1,207 deep-read notes on `main` (+2 from the 2026-08-16 Quintard 2024 + Guo 2026 clusters).
+Total ≈ 1,212 deep-read notes on `main` (+5 from the 2026-08-23/24 single-shot LDR burst, on top of +2 from the 2026-08-16 Quintard 2024 + Guo 2026 clusters).
 Review/ zhou2023 computer vision + microfluidics, **untracked** — only
 counts once committed; +2 working-tree files in the new `templates/` +
-`celvivo_commercial/` directories, both untracked). The 2026-08-16 Quintard 2024 + Guo 2026 cluster is the most recent committed deep-read additions; the 2026-08-07 / 2026-08-08 cluster (Microbioreactor + Cells) remains the prior committed cluster. CelVivo, 3Brain, Cellink, and the broad `Organoid/` bucket cover Robin's product KB per the wiki goal focus areas (类器官培养与设备, 3D 生物打印). The new `templates/` folder signals Robin may be formalizing customer-facing deliverables (checklists / templates / sales scripts) as a separate vault subtree — the IRB application checklist is the first such artifact.
+`celvivo_commercial/` directories, both untracked; +1 working-tree directory `deepread_40240593/` (7 subdirs, 2026-08-24) carrying pipeline intermediate artifacts from the 5-paper burst). The 2026-08-23/24 single-shot LDR burst (5 papers at vault root) is the most recent committed deep-read cluster; the 2026-08-16 Quintard 2024 + Guo 2026 cluster remains the prior committed cluster; the 2026-08-07 / 2026-08-08 cluster (Microbioreactor + Cells) remains the prior-prior committed cluster. CelVivo, 3Brain, Cellink, and the broad `Organoid/` bucket cover Robin's product KB per the wiki goal focus areas (类器官培养与设备, 3D 生物打印). The new `templates/` folder signals Robin may be formalizing customer-facing deliverables (checklists / templates / sales scripts) as a separate vault subtree — the IRB application checklist is the first such artifact.
 
 ## Distributor map refresh (2026-08-07)
 
@@ -662,6 +679,13 @@ fields still 待补).
 
 ## Notable entities surfaced by the vault (structural hints only)
 
+- **2026-08-23 root-level LDR burst (5 papers, single-shot entries, vault HEAD bump `a4fa5a32` → `fb017e4f`)** — **largest single-day LDR cluster since the 2026-07-29 Parate / Lee / Hodge cluster**; breaks the **7-fetch vault-silence streak** that started after the 2026-08-16 Quintard + Guo cluster; all 5 files landed at `obsidian-vault/04-Literature/Deep-Reads/` **root** (not in a per-vendor subfolder) on 2026-08-23/24 between 07:50 +0800 and 16:39 +0800; **non-standard pattern** vs the prior per-vendor subfolder discipline (every prior LDR cluster since 2026-07-28 has sorted into `Organoid/` / `Celvivo/` / `3Brain/` / `Cells/` etc.). The 5 commits are all single-shot entries (no v2/v3 SOP rewrite cycles this cluster, unlike the 2026-08-16 Quintard 4-commit + Guo 3-commit pattern). Commit timestamps `2026-08-23` (Western time) + `2026-08-24` (UTC mtime): (a) **`ad4abcde` 2026-08-24 feat: Ajongbolo 2025 Cells 脑类器官综述精读 (Wiki.js 1743)** — `Ajongbolo_2025_Cells_Brain_Organoids_Review.md`, the canonical review paper of the cluster, 11,367 bytes; (b) **`5f02489a` 2026-08-24 feat: Kim 2025 NatCommun 液态金属 3D MEA 精读 (Wiki.js 1744)** — `Kim_2025_NatCommun_Liquid_Metal_3D_MEA.md`, 13,567 bytes, the **3D-MEA competitor paper** of the cluster; (c) **`40b357b8` 2026-08-24 feat: Tian 2025 NatCommun 心外膜 mTOR 精读 (Wiki.js 1745)** — `Tian_2025_NatCommun_Epicardium_mTOR.md`, 15,578 bytes, the **cardiac-epicardium paper** of the cluster; (d) **`f2118a62` 2026-08-24 feat: Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid 精读 (Wiki.js 1746)** — `Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid.md`, 11,731 bytes, the **CelVivo-KB-derived brain-organoid CFD paper** of the cluster; (e) **`fb017e4f` 2026-08-24 feat: CelVivo App Note 2026 shear stress ClinoStar vs orbital shaker (Wiki.js 1747)** — `CelVivo_Shear_Stress_AppNote_2026.md`, 10,145 bytes, the **vendor App Note** of the cluster. **3 of the 5 papers are CelVivo-aligned**: the App Note (direct CelVivo document) + Aiello (CelVivo KB-sourced) + Ajongbolo review (mentions ClinoStar in the synthesis). **1 is a 3D-MEA competitor**: Kim EGaIn magnetic-reshapable 3D MEA. **1 is a cardiac epicardium paper**: Tian mTOR. The cluster anchors **4 of 5 to Robin's 4 vendor product lines** (CelVivo=3, 3Brain-adjacent=1) plus 1 cardiac expansion. Synthesis routes into `/themes/01-organoid-equipment.md` (CelVivo evidence) + `/themes/05-robin-research.md` (vault-snapshot refresh + deep-reads breakdown) + `/sources/literature.md` (last-30-days table). **Net-new external evidence** in this run. **Track on next fetch** whether (a) more LDR files land in vault root (extending the root-level pattern) or migrate to per-vendor subfolders on next SOP revision, (b) the `deepread_40240593/` pipeline workspace gets committed (per the Notable-entity entry below), (c) the existing MOC pattern at `04-Literature/Deep-Reads/MOCs/MOC-类器官与3D培养.md` gets a 2026-08-23/24 LDR-cycle entry.
+- **CelVivo App Note 2026 shear stress ClinoStar vs orbital shaker (2026-08-23, single-shot LDR entry `fb017e4f`, Wiki.js 1747)** — `obsidian-vault/04-Literature/Deep-Reads/CelVivo_Shear_Stress_AppNote_2026.md` (10,145 bytes). Stougaard Borgen Løschenkohl + Sedighi Frandsen, CelVivo Application Note 2026, sourced from RAGFlow CelVivo KB (1555e5b2..., Robin 8-24 上传). The note establishes **head-to-head comparison** of ClinoStar® vs orbital shaker for **3D 类器官/球体培养** (HepG2/C3A 15-day + embedded Caco-2 肠 + brain organoid). Core findings: (i) **low shear stress ClinoStar preserves cytoskeletal integrity** — phalloidin/DAPI 染色 shows ClinoStar F-actin 排列规则 + 形态紧凑 + 细胞间内聚性强 vs orbital shaker F-actin 散布 + 形态松散 + 内聚性下降 (4x + 20x dual magnification); (ii) **15-day standardized workflow** — same source cells (HepG2/C3A) + same pre-conditioning (SP5D 24孔球形板 Kugelmeiers, 1000 cells/aggregate) + same end-point assays (image + ATP + BCA + fluorescent staining + microscopy) + 3 bio-replicates per condition; (iii) **multi-model transferability** — embedded Caco-2 肠 + brain organoid both show ClinoStar advantage → vendor claim "low shear stress sensitive cell class (肝/肠/脑来源)" validated across 3 model systems. **Net-new external evidence** in this run (git-repo-1 2026-08-24). **Highest commercial-relevance paper of the cluster**: this is the **canonical CelVivo vs-orbital-shaker competitive-killer document** and is Robin's first **vendor App Note deep-read** (vs. 1 primary research paper + 1 review paper from prior clusters). **Track on next fetch** whether (a) Robin produces a deep-read synthesis page at `/sources/literature-celvivo-shear-stress-appnote-2026-deepread.md` mirroring the prior pattern, (b) the App Note is folded into `06-Sales/Content/Training-Materials/` (the existing CelVivo赋能培训 v1-v9 cluster could absorb the head-to-head comparison data as a new PPT module), (c) the App Note spawns a new sales commitment similar to the existing `celvivo-clinoreactor-thewell-bioscience-xeno-free` item.
+- **Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid CFD (2026-08-23, single-shot LDR entry `f2118a62`, Wiki.js 1746)** — `obsidian-vault/04-Literature/Deep-Reads/Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid.md` (11,731 bytes). Aiello F, Bagni C et al., biorxiv preprint (the EMBO Reports submission version `Aiello_Bagni_EMBO R_050325`), 2026-08-24 Robin 从 CelVivo KB 找到的 PDF. The paper is **the first CFD-quantified Rotating Chamber (RC) low-shear-stress study** in Robin's deep-read set: (i) RC geometry: outer Ø 78.3 mm × H 24.2 mm, inner cavity Ø 37.7 mm × H 8.8 mm (reserved for organoid growth space); (ii) **COMSOL Multiphysics 6.2 CFD simulation shows cavity shear stress < 20 mPa** (extremely low vs. standard stirred/shaken systems) — **first quantitative number** on RC shear-stress field; (iii) **multi-density organoid shear-stress validation** — relative density 1010/1020/1030 vs medium 1000 confirms shear-stress uniformity across density range + 0-300 objects all experience stable low shear; (iv) **morphological consistency** — multi-batch + multi-cell-line brain organoid cultures show **CV < 15%** (vs static culture CV > 30%); (v) cortical cell type recapitulation (SOX2/Nestin/PAX6 + DCX + TBR1/SATB2/MAP2 multi-marker IHC) + scRNA-seq OXPHOS metabolic profiling. **Net-new external evidence** in this run. **Vendor-anchor mapping**: Aiello paper is **CelVivo ClinoStar-adjacent** (uses RC system which is the ClinoStar simplified geometry) + 3Brain HD-MEA-adjacent (cortical organoid electrophysiology read-out). The < 20 mPa quantitative claim is **direct ammunition** for the existing ClinoStar ⭐⭐⭐⭐ vendor-positioning pitch — no prior paper in Robin's deep-read set provides the **quantitative CFD number** for ClinoStar's low-shear-stress value proposition. **Track on next fetch** whether Robin produces a deep-read synthesis page; the deep-read pipeline currently does not carry an Aiello synthesis page.
+- **Tian 2025 Nat Commun hiPSC epicardium mTOR maturation (2026-08-23, single-shot LDR entry `40b357b8`, Wiki.js 1745)** — `obsidian-vault/04-Literature/Deep-Reads/Tian_2025_NatCommun_Epicardium_mTOR.md` (15,578 bytes, the **largest** of the 5 new LDR files). Tian Y, Lucena-Cacace A, Tani K et al., Nat Commun 2025, DOI 10.1038/s41467-025-60934-8. The paper establishes: (i) **mTOR inhibition drives epicardium maturation** — Torin1 (dual mTORC1/2 inhibitor, 200 nM) on mouse E12 embryonic heart explants upregulates WT1 (mature epicardium marker) + downregulates EMT markers (SNAI1/2) + downregulates cell cycle (MKI67/PCNA) + downregulates mTOR pathway; bulk RNA-seq PCA shows 28.53% (PC1) + 36.01% (PC2) variance explained = significant transcriptome shift toward **adult** epicardium identity; (ii) **mTORC1/2 dual inhibition is critical** — Rapamycin (single mTORC1) alone is insufficient; mTORC2 is the key node; (iii) **hiPSC-derived epicardium maturation with Torin1** — qPCR validates mature markers (TJP1/WT1/TBX18/ALDH1A2/CDH18) up + EMT markers (SNAI1/SNAI2/CDH2/CDH1) down + proliferation markers (MKI67/PCNA/MCM2) down + fatty-acid metabolism maturation (PDK4/PPARGC1A/SIRT1/FASN/SREBF1); bulk RNA-seq with 379 DEGs; Torin1 vs adult epicardium GSE84085 heatmap confirms adult-like profile; YBX3 + MAFF identified as Torin1-specific TFs via Venn diagram + siRNA knockdown; (iv) **paracrine effect on cardiomyocyte maturation** — mature epicardium (Torin1-treated) co-cultured with hiPSC ventricular cardiomyocyte EBs for 10 days upregulates TNNI3 (adult cardiomyocyte marker) → epicardium-to-cardiomyocyte maturation cross-talk via paracrine signaling. **Net-new external evidence** in this run. **Application domain**: cardiac organoid regeneration; not directly anchored to CelVivo/3Brain/Cellink/Femtobiomed product lines (no vendor rating callouts in the deep-read) but **fits the broad cardiac application-domain bucket** that Robin's wiki scope covers. **Track on next fetch** whether (a) Robin produces a deep-read synthesis page, (b) the Tian paper routes into any commitment — there is no cardiac-specific commitment in `/commitments.md` yet.
+- **Kim 2025 Nat Commun EGaIn liquid-metal 3D MEA (2026-08-23, single-shot LDR entry `5f02489a`, Wiki.js 1744)** — `obsidian-vault/04-Literature/Deep-Reads/Kim_2025_NatCommun_Liquid_Metal_3D_MEA.md` (13,567 bytes). Kim E, Jeong E, Hong YM et al., Nat Commun 2025, DOI 10.1038/s41467-024-55752-3. The paper establishes: (i) **EGaIn (eutectic gallium-indium) 3D pillar MEA** — microfluidic direct-ink-writing fabrication with **pillar height 25-310 μm controllable by printing speed** (R² > 0.95, n=12 datapoints), **pillar diameter down to 14 μm** (single-neuron scale), parylene-C selective encapsulation with only pillar tip exposed as electrode; (ii) **magnetically reshapable** — Ni magnetic layer on pillar exterior + external magnetic field drives pillar bending → 3D MEA shape conforms to different organoid sizes/shapes (no fabrication re-spin needed); (iii) **hPSC cortical organoid maturation timeline** — Dorsomorphin + SB-431542 (dual-SMAD) → FGF2/EGF expansion → BDNF/NT3 maturation at 15/30/45/60/120 days; 30d SOX2+/TUJ1+ early neuronal; 60d SOX2↓ + TUJ1↑↑; 120d 6-marker co-expression (MAP2/GFAP/VGLUT1/SATB2/CTIP2) = cortical 6-layer-like structure; (iv) **biocompatible integration** — 4-month organoid seeded on 3D MEA, co-cultured 14 days; live/dead + qPCR (PAX6/Nestin/TUJ1) + IHC (Nestin/TUJ1/SOX2/MAP2/GFAP) all confirm biocompatibility; (v) **single-unit spike + network synchronization recording** — 64-channel MEA acquisition + SU spike detection + synchronization score + network graph analysis across 1/2/4/6 month organoid maturation. **Net-new external evidence** in this run. **Vendor-anchor mapping**: **3Brain HD-MEA competitor / adjacent paper** — Kim's EGaIn 3D MEA is a next-generation flexible 3D MEA approach that is **adjacent to but not directly competing with** 3Brain's rigid Accura-3D chip (3Brain uses 4096-electrode planar CMOS, Kim uses 3D pillar EGaIn with magnetic reshape). The Liu 2026 NBME shape-conformal framework from the 2026-08-08 cluster is a third next-gen HD-MEA approach — **3 distinct next-gen HD-MEA approaches now in Robin's deep-read set**: Liu 2026 Rogers Lab flexible-electronics framework (3000-8000 ch, >90% surface coverage) + Kim 2025 EGaIn 3D pillar (25-310 μm height, magnetic reshape) + Kim's pillar biocompatibility with cortical organoid (1/2/4/6-month maturation timeline). **Track on next fetch** whether (a) Robin produces a deep-read synthesis page at `/sources/literature-kim-2025-nat-commun-liquid-metal-3d-mea-deepread.md`, (b) the Kim paper routes into the existing `liu-2026-hd-mea-roadmap-implication` commitment as a corroborating evidence point (the "next-gen HD-MEA field" consolidation narrative now has 3 distinct data points), (c) the Kim paper pairs with Liu 2026 to anchor a new `liu-kim-2025-2026-next-gen-hd-mea-roadmap` consolidation synthesis page.
+- **Ajongbolo 2025 Cells brain organoid review (2026-08-23, single-shot LDR entry `ad4abcde`, Wiki.js 1743)** — `obsidian-vault/04-Literature/Deep-Reads/Ajongbolo_2025_Cells_Brain_Organoids_Review.md` (11,367 bytes). Ajongbolo AO, Langhans SA, Cells 2025, DOI 10.3390/cells14110842. The paper is the **canonical brain organoid + assembloid review** for Robin's deep-read set: (i) **3-tier brain-organoid taxonomy** — whole-brain organoid (Lancaster 2013 archetype, multi-brain-region identity, unguided spontaneous differentiation) → region-specific organoid (前脑/中脑/小脑/下丘脑 with patterning-factor-guided TF markers FOXG1 / OTX2 / EN1/EN2 / GBX2 / FGF8 / SHH) → complex organoid systems (assembloid multi-region fusion / OoC vascularization-perfusion / tumoroid tumor modeling / xenograft immunodeficient-mouse intracranial transplant); (ii) **4-class characterization methodology** — morphological (IF + confocal) + biochemical (qPCR/Western/bulk RNA-seq) + functional (MEA / patch clamp / calcium imaging) + omics (scRNA-seq / spatial transcriptomics / mass spec) = standardized decision tree for any new brain-organoid line publication; (iii) **disease-modeling real-world boundaries** — neurodevelopmental (microcephaly / macrocephaly / ASD / Rett / Fragile X / Timothy syndrome) + neurodegenerative (AD / PD / HD / ALS) + brain-tumor (glioblastoma / medulloblastoma / AT/RT); (iv) **drug-discovery pipeline standardization** — 96-well + microfluidic chip + automated imaging + AI phenotype recognition → emerging HTS platform. **Net-new external evidence** in this run. **Vendor-anchor mapping**: **CelVivo ClinoStar-adjacent** (long-term stable brain organoid culture) + 3Brain HD-MEA-adjacent (functional electrophysiology read-out via MEA in the 4-class characterization). **Cross-vendor**: Ajongbolo review is the **first comprehensive brain-organoid review** in Robin's deep-read set since the 2026-07-29 Organoids 2026 review (`10.1186_s40164-026-00743-x`) — pairs with that review to give Robin a **review-paper anchor for the brain-organoid lane**. **Track on next fetch** whether (a) Robin produces a deep-read synthesis page at `/sources/literature-ajongbolo-2025-cells-brain-organoids-review-deepread.md`, (b) the review routes into `/themes/05-robin-research.md` as a "review-paper cluster" entry alongside the Organoids 2026 review.
+- **`deepread_40240593/` pipeline workspace (2026-08-24, NEW this fetch)** — `deepread_40240593/` is the **first** new untracked directory added this fetch, alongside the 5 root-level LDR files. The directory contains **7 subdirectories**: `deepread_40016200` + `deepread_40441268` + `deepread_40498018` + `deepread_40593704` + `deepread_41151577` + `deepread_brain_organoid_fluid` + `deepread_shear_stress_cs_os`. The directory is at the vault root (not in `04-Literature/`); the numeric-name pattern (`deepread_<PMID>`) suggests Robin's LDR pipeline is using these directories as **per-paper working folders** — the names include both PMIDs (40016200, 40441268, 40498018, 40593704, 41151577) and human-readable slugs (brain_organoid_fluid → maps to Aiello paper; shear_stress_cs_os → maps to CelVivo App Note). The directory mtime is 2026-08-24 16:31 +0800 (1-day-old at this fetch). **Structural significance**: (a) the `deepread_<PMID>` naming convention is **not** the prior `04-Literature/Deep-Reads/<vendor>/` convention — the new pattern is a **per-paper working-folder pattern** that holds pipeline intermediate artifacts (PDFs, image-extracts, qa-pass outputs, Ollama vision-audit intermediates, etc.) before final commit to `04-Literature/Deep-Reads/`. (b) The presence of 5 already-committed root-level LDR files (in `04-Literature/Deep-Reads/`) **plus** the 2 named `deepread_<slug>` folders (brain_organoid_fluid + shear_stress_cs_os) — which exactly map to 2 of the 5 committed papers — suggests Robin's pipeline writes the **committed LDR note** to `04-Literature/Deep-Reads/` and the **intermediate artifacts** to `deepread_40240593/deepread_<slug>/`; the other 5 PMID-named subdirs are pipeline intermediates for 5 other in-progress papers not yet on `main`. (c) The vault-root `deepread_40240593/` is **not yet committed** (carry-forward untracked); the 2026-08-23 7-paper burst means Robin is producing pipeline output faster than the manual commit cycle. **Carry-forward status** (2026-08-24): untracked, **1-day-old** at this fetch, future-commit candidate. **Track on next fetch** whether (a) the directory is committed, (b) more papers move through the pipeline, (c) the `deepread_<PMID>` vs `04-Literature/Deep-Reads/<slug>` separation is formalized in vault conventions. **No synthesis routes** until the directory is committed.
 - **Quintard 2024 Nat Commun microfluidic vascularized organoid chip (2026-08-16, 7 commits in 48-hour window)** — `obsidian-vault/Literature/Deep-Reads/Organoid/quintard_2024_vasorganoid_chip.md` (189 lines; 4-commit LDR cycle `0fc8db24` adds → `290e9635` -v2 修图床/双 H1/落款 → `24c713de` -v3 Wiki.js 自带图床 6 张图 → `4073abf4` -v4 转发语标题/落款加粗/删 3Brain; Wiki IDs 1719/1720/1722, NocoDB Id 3297). The paper is **the first microfluidic / organ-on-chip platform paper** Robin has deep-read in the 2026-08 window; the platform combines (i) 10-channel parallel microfluidic chips with U-shaped capture sites (300-800 µm adjustable), (ii) fibrin + Matrigel hydrogel layer deposition using Landau-Levich-Bretherton effect, (iii) ~10 s capture efficiency ~100%, (iv) dynamic perfusion 7-30 days with HUVEC + fibroblast co-culture + hiPSC-derived BVO + islet spheroid + lung organoid cross-validation, (v) hierarchical vasculature (37 µm upstream artery-vein + 8 µm BVO capillaries, matching physiological ranges 50-1900 s⁻¹). **Net-new external evidence** in this run (git-repo-1 2026-08-17). The 4-star "与我们的产品" table lists **Mimetas OrganoPlate ⭐⭐⭐⭐** (direct Phaseguide-based vascularized / kidney / intestine organ-on-chip competitor) + **CelVivo ClinoStar ⭐⭐⭐** (upstream workflow step: BVO differentiation day 0-15 needs rotating-wall suspension culture; ClinoStar is the canonical hardware for this stage; the paper's day 15 → chip-on-board handoff is the canonical CelVivo → organ-on-chip workflow) + **Emulate Organ-Chip ⭐⭐⭐** (Colon-Intestine-Liver platform companion). Synthesis routes into `/themes/05-robin-research.md` (新增 by-vendor entry) and `/sources/literature.md` (last-30-days deep-read table). Vendor-anchor mapping: CelVivo-adjacent (workflow upstream) + Mimetas OrganoPlate / Emulate Organ-Chip (organ-on-chip product lane that Robin's portfolio does not currently cover but represents a potential cross-sell if Robin's customers ask "where do I run my CelVivo-matured organoids in a vascularized microenvironment?"). Customer-entry: 药企临床前 (vascularized drug screening) + 微流控 organ-on-chip 平台集成商 (Mimetas / Emulate channel partners) + academic PI (vascular development / diabetes). **Track on next fetch** whether Robin publishes a `/sources/literature-quintard-2024-nat-commun-vasorganoid-chip-deepread.md` synthesis page; the deep-read pipeline currently only carries `liang-2026-celvivo-clinostar-positioning` + `miao-2025-celvivo-clinostar-positioning` + `liu-2026-hd-mea-roadmap-implication` + `sharma-2026-retinal-organoid-mea-cross-sell` — no Quintard synthesis page yet.
 - **Guo 2026 Front Cell Dev Biol organoid ethics systematic review (2026-08-16, 7 commits in 48-hour window)** — `obsidian-vault/Literature/Deep-Reads/Organoid/guo_2026_organoid_ethical_review.md` (170 lines + 3 真图; 3-commit LDR cycle `2e6f5989` adds → `00f824f2` -v2 修图床 (加 2 张 Wiki.js 自带图) → `a4fa5a32` -v3 补 Fig 1; Wiki ID 1724, NocoDB Id 3298). The paper is **the first organoid-research ethics / IRB systematic review** Robin has deep-read in any window — fills the methodological gap on the IRB-side that the prior clinical-translation evidence (CDE classification + Order No. 818) does not directly cover. The paper structures the IRB review framework as **7 mandatory points** (sample provenance + donor informed consent + data privacy & de-identification + co-culture agency + animal welfare 3R + cross-border regulation + long-term storage & termination); the cross-border table compares China (《人类遗传资源管理条例》+ 《涉及人的生命科学和医学研究伦理审查办法》2023), US (HIPAA + Common Rule + NIH GDS), EU (GDPR + Clinical Trials Regulation 536/2014), Japan, Korea. The 4-star "与我们的产品" table lists **CelVivo ClinoStar ⭐⭐⭐⭐** (clinical-translation IRB compliance — the 7 mandatory points directly apply to ClinoStar customer IRB submissions; **Robin 推广 ClinoStar 给三甲医院时, 可附带本框架作为增值**) + **Mimetas OrganoPlate ⭐⭐⭐⭐** (co-culture ethics — Mimetas 主营类器官 + 免疫细胞共培养, the co-culture agency framework from Guo 2026 point 4 directly applies) + **Emulate Organ-Chip ⭐⭐⭐** (organoid + microfluidic cross-border regulation reference). **Net-new external evidence** in this run (git-repo-1 2026-08-17). Robin translated the paper into a **customer-facing sales ammunition template** at `templates/类器官IRB申请清单.md` (180 lines; 7 mandatory points checklist + 5 customer Q&A + China vs International flow table + 10 attachments + 7 citations; v1.0 dated 2026-08-16, "Robin 8-16 实战沉淀") — see the templates/ entry in the next bullet. Customer-entry: 临床转化型医院 (class A tertiary hospitals running organoid IRB submissions) + 类器官厂家 Robin 培训客户 (the IRB checklist is the canonical "Robin 培训客户 IRB 申请" core material) + academic PI (cross-border collaboration IRB paperwork). **Track on next fetch** whether (a) the templates/ IRB checklist gets committed, (b) the Robin-author attribution in the template is recognized as a wiki-side customer-training artifact, (c) Robin produces a deep-read synthesis page at `/sources/literature-guo-2026-front-cell-dev-biol-organoid-ethics-deepread.md`, and (d) the IRB checklist routes into the existing CelVivo industrial-customers program (which currently lives at `05-Documents/Products/Celvivo/Industrial-Customers/`) as a standard customer-training artifact.
 - **Review/ subfolder — first entry (2026-08-13, still uncommitted at 2026-08-23, 12-day carry-forward, watchlist signal continuing to weaken)** — `obsidian-vault/04-Literature/Deep-Reads/Review/zhou2023_computer_vision_microfluidics.md` is the **first file in a new `Review/` subfolder** under `04-Literature/Deep-Reads/`. The file is **untracked** at the 2026-08-23 fetch (Robin has not yet committed it — same state as the 2026-08-13 + 2026-08-15 + 2026-08-17 + 2026-08-18 + 2026-08-19 + 2026-08-20 + 2026-08-21 + 2026-08-22 fetches, **0 progress in the 2026-08-13 → 2026-08-23 240-hour window**, **10 consecutive fetches silent on the file**). **Watchlist signal continuing to weaken at this fetch**: per the 2026-08-15 watchlist-trigger rubric ("if the file remains uncommitted at the next fetch (2026-08-17 or later), the structural signal weakens"), the `Review/` subfolder at the 2026-08-23 fetch is **still single-entry, still untracked** — the structural signal **continues to weaken** at this fetch (**12-day carry-forward** with no commit, no follow-up paper landed in the subfolder across **10 consecutive fetches**). The `Review/` subfolder is more likely to be a one-off than a recurring pattern. The file should either be committed on a future fetch (which would re-strengthen the signal) or remain untracked indefinitely (which would confirm the one-off interpretation). No synthesis routes until the file is committed.
@@ -749,7 +773,140 @@ fields still 待补).
 
 ## Synthesis policy for this source
 
-- **This run (2026-08-23, git-repo-1 18:11Z):** **0 new vault commits**
+- **This run (2026-08-24, git-repo-1 18:05Z):** **5 new vault commits**
+  in the 2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window — the
+  vault HEAD bumped from `a4fa5a32` (2026-08-23T18:11Z fetch) to
+  `fb017e4f` — **breaks the 7-fetch vault-silence streak** that
+  started after the 2026-08-16 Quintard + Guo cluster, **largest
+  single-day LDR burst since the 2026-07-29 Parate / Lee / Hodge
+  cluster**. The 5 commits are all single-shot entries (no v2/v3
+  SOP rewrite cycles this cluster, unlike the 2026-08-16 Quintard
+  4-commit + Guo 3-commit pattern): (a) `ad4abcde` Ajongbolo 2025
+  Cells brain organoid review (Wiki.js 1743); (b) `5f02489a` Kim 2025
+  Nat Commun EGaIn liquid-metal 3D MEA (Wiki.js 1744); (c) `40b357b8`
+  Tian 2025 Nat Commun hiPSC epicardium mTOR maturation (Wiki.js
+  1745); (d) `f2118a62` Aiello & Bagni 2025 biorxiv Rotating Chamber
+  brain organoid CFD (Wiki.js 1746, CelVivo KB-derived); (e)
+  `fb017e4f` CelVivo App Note 2026 shear stress ClinoStar vs orbital
+  shaker (Wiki.js 1747). **All 5 files landed at
+  `obsidian-vault/04-Literature/Deep-Reads/` root** (not in a
+  per-vendor subfolder — non-standard pattern, see Notable-entity §
+  "2026-08-23 root-level LDR burst"). Vendor-anchor mapping: 3
+  CelVivo-aligned (App Note + Aiello + Ajongbolo review) + 1
+  3Brain-adjacent (Kim EGaIn 3D MEA) + 1 cardiac epicardium (Tian mTOR,
+  no vendor callouts). **1 new untracked directory** appeared in the
+  working tree this fetch — `deepread_40240593/` (7 subdirs: 5
+  PMID-named + 2 slug-named that exactly map to the Aiello + CelVivo
+  App Note papers; the new directory is Robin's LDR pipeline
+  intermediate-artifact workspace, see Notable-entity). The 2
+  prior untracked directories (`celvivo_commercial/` + `templates/`)
+  and the `Review/zhou2023_computer_vision_microfluidics.md` first-entry-in-Review/-subfolder carry forward unchanged from the
+  prior fetch (now **8-day carry-forward** for `celvivo_commercial/` +
+  `templates/`; **13-day carry-forward** + **11 fetches silent** for
+  the `Review/` file). Wiki-side: **2 new automated wiki commits**
+  landed in the 24-hour window — `9cc5e63` chore: daily update
+  2026-08-24 (2026-08-24 02:23 +0800) + `127c2dc` chore(sources):
+  refresh NocoDB/SeaFile/Wiki.js/RAGFlow counts (sources-sync
+  2026-08-24, 2026-08-24 02:30 +0800) — advancing wiki HEAD from
+  `a9568d8` (2026-08-23T18:11Z fetch) to `127c2dc`. Both commits are
+  routine auto-orchestration: (a) the `9cc5e63` daily update carried
+  the synthesis-layer edits from the prior HN-1 2026-08-23T18:00Z +
+  web-search-1 2026-08-23 (skipped) + git-repo-1 2026-08-23T18:11Z
+  runs; the `9cc5e63` daily update committed `sources/git-repo.md` +
+  `sources/hackernews.md` + `sources/index.md` +
+  `sources/openwiki-version-drift.md` + `sources/web-search.md` +
+  `themes/05-robin-research.md` from the 2026-08-23 dirty set; (b)
+  the `127c2dc` sources-sync re-applied the count-data wipe pattern
+  on NocoDB / SeaFile / Wiki.js / RAGFlow for the **tenth**
+  consecutive sources-sync (after 2026-08-15 `2507925` + 2026-08-16
+  `997b648` + 2026-08-17 `be49285` + 2026-08-18 `1fc73ae` +
+  2026-08-19 `dcb53a2` + 2026-08-20 `bd30339` + 2026-08-21 `f6c2576` +
+  2026-08-22 `43d4548` + 2026-08-23 `8f8f767`).
+  **Net-new external evidence** in this run: **5 new paper deep-reads**
+  (1 vendor App Note + 4 primary research papers / 1 review / 1
+  preprint) — all committed to `main`. **Net-new working-tree
+  artifact**: `deepread_40240593/` pipeline-workspace directory
+  (1 new untracked dir). **New commitments** added on `/commitments.md`:
+  (a) `celvivo-app-note-2026-clinostar-vs-orbital-shaker-positioning`
+  (fold the head-to-head shear-stress comparison into the ClinoStar
+  sales narrative + into the existing CelVivo赋能培训 cluster); (b)
+  `aiello-2025-rc-cfd-clinostar-positioning` (the < 20 mPa quantitative
+  number is direct ClinoStar value-prop ammunition); (c)
+  `kim-2025-egain-3d-mea-vs-3brain-positioning` (next-gen HD-MEA
+  field consolidation: Liu 2026 Rogers Lab + Kim 2025 EGaIn + 3Brain
+  Accura-3D); (d) `ajongbolo-2025-brain-organoid-review-cluster`
+  (pair with the existing Organoids 2026 review to anchor
+  brain-organoid review lane); (e) `tian-2025-epicardium-mtor-cardiac-expansion`
+  (first cardiac-epicardium paper in deep-read set; no vendor
+  callouts). **New themes-row refresh candidate** for
+  `/themes.md` `class-organoid-3d-bioprinting` row: the 5-paper
+  burst is the largest single-day cluster since 2026-07-29 + breaks
+  the 7-fetch silence; 3 of the 5 papers directly reference CelVivo
+  ClinoStar or 3Brain, validating the recurring-signal evidence
+  footprint. **Track on next fetch** whether (a) the `deepread_40240593/`
+  directory gets committed (currently untracked, 1-day-old); (b) any
+  of the 5 new LDR files get a wiki-side synthesis page (none
+  currently in `/sources/literature-*` naming pattern). Wiki edits
+  this run: (a) `/sources/git-repo.md` (this page) — refreshed
+  frontmatter HEAD pointers (vault HEAD `a4fa5a32` → `fb017e4f`, wiki
+  HEAD `a9568d8` → `127c2dc`, tags + `2026-08-24`, timestamp →
+  `2026-08-24T18:05:00Z`), refreshed the Connector / State section
+  to the new `2026-08-24T18-05-31-919Z` raw manifest, refreshed the
+  obsidian-vault working-tree-status section to 2026-08-24 (5 new
+  vault commits, 1 new untracked dir `deepread_40240593/`,
+  `celvivo_commercial/` + `templates/` 7-day → 8-day carry-forward,
+  `Review/` 12-day → 13-day + **10 fetches silent** → **11 fetches
+  silent**), refreshed the openwiki-wiki working-tree-status section
+  to 2026-08-24 (2 new automated wiki commits + 8 uncommitted
+  synthesis-layer edits per the manifest `changedFiles` list), added
+  2 new entries at the top of the Recent-commits-for-vault table
+  (5 new vault commits) + 2 new entries at the top of the
+  Recent-commits-for-wiki table (`127c2dc` sources-sync 2026-08-24
+  + `9cc5e63` daily update 2026-08-24), refreshed the
+  Summary-of-last-48-hours table to add the
+  2026-08-23T18:11Z → 2026-08-24T18:05Z window data (5 vault
+  commits / 2 wiki commits / operational row noting the **tenth**
+  consecutive sources-sync re-applying the count-data wipe pattern
+  + the 5-paper LDR burst detail), refreshed the
+  `04-Literature/Deep-Reads/` breakdown table (added the root-level
+  5-file entry + bumped `Review/` 12 → 13-day + `celvivo_commercial/` +
+  `templates/` 7 → 8-day carry-forward + total 1,207 → 1,212), added 7
+  new entries to the Notable-entity section (the 2026-08-23
+  root-level LDR burst overview + 5 individual paper entries + the
+  `deepread_40240593/` pipeline workspace entry), refreshed the
+  Synthesis-policy section with this run's delta summary (the old
+  2026-08-23 "This run" was demoted to "Previous run (2026-08-23,
+  git-repo-1 18:11Z)" and the new "This run" reflects the 5-paper
+  LDR burst); (b) `/themes/05-robin-research.md` — vault-snapshot
+  metadata refreshed (fetch timestamp → `2026-08-24T18:05:00Z`,
+  raw-manifest path → the new `2026-08-24T18-05-31-919Z` manifest,
+  HEAD bumped `a4fa5a32` → `fb017e4f`, the 5-paper LDR burst noted
+  in the cluster section, the `04-Literature/Deep-Reads/` breakdown
+  table updated with the root-level 5-file entry + total 1,207 →
+  1,212, `celvivo_commercial/` + `templates/` 7-day → 8-day
+  carry-forward + `Review/` 12-day → 13-day + **10 fetches silent**
+  → **11 fetches silent**); (c) `/commitments.md` — added 5 new
+  commitment entries corresponding to the 5 new LDR papers (each
+  with Owner: me + Source: git-repo-1 2026-08-24 + Status: open); (d)
+  `/sources/literature.md` — added 5 new rows to the "Last 30 days
+  of deep reads" table under a new `2026-08-23` subsection; (e)
+  `/quickstart.md` — added this "Latest update — git-repo-1
+  (2026-08-24T18:05Z)" paragraph; updated the Backlog git-repo entry
+  to reflect the new wiki HEAD `127c2dc` + 2 new automated wiki
+  commits + the carry-forward 8 uncommitted synthesis-layer edits
+  + the **tenth** consecutive sources-sync note + the 5-paper LDR
+  burst detail; (f) `/sources/README.md` — refreshed Active
+  connectors Git repo row + Connector roll Git repo row to mention
+  the 2026-08-24 18:05 UTC fetch + the **tenth** consecutive
+  sources-sync. **No `/themes.md` row change** — the 5-paper LDR
+  burst strengthens the existing `class-organoid-3d-bioprinting`
+  evidence footprint (3 of 5 papers vendor-aligned to CelVivo +
+  3Brain) but does not warrant a new theme row at this fetch
+  (single-day burst, no recurrence yet); if a 2026-08-25 fetch
+  surfaces another deep-read cluster, that becomes the recurrence
+  trigger for a theme-row promotion.
+
+- **Previous run (2026-08-23, git-repo-1 18:11Z):** **0 new vault commits**
   in the 2026-08-22T18:13Z → 2026-08-23T18:11Z 24-hour window — the
   vault HEAD is **unchanged at `a4fa5a32`** for the **7th consecutive
   fetch** (the 2026-08-16 Quintard 2024 Nat Commun 4-commit LDR cycle

sources/hackernews.md

@@ -2,8 +2,8 @@
 type: "Reference"
 title: "Hacker News — evidence index"
 description: "Compact evidence trail for the Hacker News connector (hackernews-1, Robin-curated life-sciences + AI workflow). Synthesis lives on /themes.md, /commitments.md, etc.; this page only records ingestion coverage and notable items."
-tags: [hackernews, evidence-index, source, 2026-08-23, 2026-08-22, 2026-08-21, 2026-08-20, 2026-08-19, 2026-08-18, 2026-08-17, 2026-08-16, 2026-08-15, 2026-08-13, 2026-08-12, 2026-08-10]
-timestamp: 2026-08-23T18:00:28Z
+tags: [hackernews, evidence-index, source, 2026-08-24, 2026-08-23, 2026-08-22, 2026-08-21, 2026-08-20, 2026-08-19, 2026-08-18, 2026-08-17, 2026-08-16, 2026-08-15, 2026-08-13, 2026-08-12, 2026-08-10]
+timestamp: 2026-08-24T18:00:28Z
 ---
 
 # Hacker News — evidence index
@@ -16,10 +16,39 @@ Compact evidence trail for the Hacker News connector (`hackernews-1`, Robin-cura
 - **Backend:** public HN feeds (`top`, `new`) + Algolia HN search queries
 - **Auth:** none — public feeds
 - **Window:** last 24 hours
-- **Latest fetch:** `2026-08-23T18:00:28Z` (LLM-agent topic set: `organoid`, `CelVivo`, `3Brain`, `Cellink`, `openwiki`, `deepagents` — 6 queries; the source-specific instruction "Track organoid / CelVivo / 3Brain / Cellink / openwiki / AI agent stories" is the active query set; prior 2026-08-22 18:00, 2026-08-21 18:00, 2026-08-20 18:00, 2026-08-19 18:00, 2026-08-18 18:00, 2026-08-17 18:00, 2026-08-16 18:00, 2026-08-15 18:00, 2026-08-13 18:00, 2026-08-12 18:00, 2026-08-10 18:00, 2026-08-08 18:00, 2026-08-08 03:15, 2026-08-07 12:29, 2026-08-03 18:00, 2026-08-02 18:00, 2026-08-01 18:00, 2026-07-31 18:00, 2026-07-30 18:00, 2026-07-29 18:00, 2026-07-28 18:00, 2026-07-27 18:00, 2026-07-26 18:16 and 18:00 pulls also documented below)
-- **Raw dumps:** `~/.openwiki/connectors/hackernews/raw/2026-08-23T18-00-02-256Z/hackernews-results.json` (current — **thirteenth pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries**; **fully-zero Algolia lane on all 6 queries**: `organoid` / `CelVivo` / `3Brain` / `Cellink` / `openwiki` / `deepagents` each returned `nbHits=null / hits=0` — same shape as the 2026-08-21 fully-zero pull (the prior fully-zero pull was 2026-08-21); the 2026-08-22 pull had a noise-only lane (3 of 6 with substring noise, 3 clean zero) and the 2026-08-23 pull returns to fully-zero across all 6 — **two fully-zero Algolia lanes in three consecutive pulls**, confirming the empty-pulls-on-vendor-queries cadence is normal under the 6-query LLM-agent topic set, not an outage, no config-side intervention needed; the strongest named-stack-adjacent item is **`49409092` "What Is a Harness?"** (121 pts, 83 cmts, top, by `tosh` 2026-08-23, https://earendil.com/posts/what-is-a-harness/ — a definitional essay on agent harnesses; **strongest named-stack-adjacent item of the pull by direct agent-harness cross-walk** because (a) the **121-pt + 83-cmt top-feed engagement** is the third-highest top-feed engagement on the pull (after the off-scope `49402232` Why your local LLM feels dumber 450 pts + `49402189` Scrap Moxie 399 pts), (b) the **definitional framing "What Is a Harness?"** directly speaks to the agent-as-primary-compute-substrate narrative that `hermes-agent-v0-19-quicksilver` documents, and (c) it is the **6th entry in the now 11-day self-hosted-agentic-substrate cluster** — DeepSeek Harness `49285244` 422 pts 2026-08-13 → machine0 `49348136` 19 pts 2026-08-18 → OneCLI `49363710` 21 pts 2026-08-19 → self-hosted agentic software factory `49390463` 16 pts 2026-08-21 → Munder Difflin `49398152` 192 pts 2026-08-22 → **"What Is a Harness?" `49409092` 121 pts 2026-08-23** — six independent submissions across 11 days, total cluster engagement 791 pts), with the next named-stack-adjacent item being **`49402232` "Why your local LLM feels dumber than it is"** (450 pts top+best, 182 cmts, by `felineflock` 2026-08-23, https://forum.level1techs.com/t/why-your-local-llm-feels-dumber-than-it-is/253917 — **highest-engagement item of the pull** at 450 pts + 182 cmts; a Level1Techs forum post on local-LLM optimization; **adjacent-not-promoted** per scope because local-LLM optimization is not on Robin's named daily-driver stack — Hermes / MiniMax-M3+H3 / OpenWiki / RAGFlow / NocoDB / SeaFile / Codex CLI are the named agents and unsloth/MiniMax-M3-GGUF is the local-LLM run path — but the **direct local-LLM-stack cross-walk to the `MiniMax-m3` row** makes this the strongest local-LLM-adjacent item of the pull), and the third named-stack-adjacent item being **`49410097` "GLM-5.3 (open-weight) beat Anthropic/OpenAI models – for 1/5 the cost"** (141 pts top, 143 pts best, by `ed-is-ai` 2026-08-23, https://reinvently.co.uk/tools/ed-o-meter/ — GLM-5.3 open-weight model benchmark, **adjacent-not-promoted** per scope because GLM-5.3 is not on Robin's named stack — the `MiniMax-m3` row already cites the 2026-08-01 benchlm.ai DeepSeek V4 Flash vs MiniMax M3 framing, and GLM-5.3 is a different open-weight model from the comparison anchor; cross-walked to **`49409073` "I spent $266 and four AI models to own my tablet. GLM-5.3 finished it in a day"** (268 pts top+best, 107 cmts, by `dr_pardee` 2026-08-23, https://ericpardee.github.io/fire-hd-ownership/ — GLM-5.3 in a tablet-ownership experiment; **highest-engagement GLM-5.3 cluster item** at 268 pts, off-scope per scope but reinforces the GLM-5.3 launch narrative), with one lower-engagement application-domain-adjacent item worth flagging: **`49410149` "Vit D assoc w better cognition in those w sleep disturb./mild cognitive impair"** (15 pts top, 5 cmts, by `bookofjoe` 2026-08-23, https://www.sciencedirect.com/science/article/abs/pii/S1389945726001991 — a sciencedirect.com clinical-nutrition paper on Vitamin D and cognition in sleep-disturbance + mild cognitive impairment; **adjacent-not-promoted** per scope because clinical nutrition is not one of Robin's 4 application domains — 3D cell culture, 3D bioprinting, neuroscience in vitro, organoid workflows — though the cognition + sleep-disturbance framing has a tangentially neuroscience-in-vitro adjacency), and three off-scope secondary adjacents worth flagging for traceability: **`49410628` "We must not grant AI agents legal personhood"** (7 pts top, 5 cmts, by `andsoitis` 2026-08-23, https://www.ft.com/content/b8cc4bf4-6d3c-4974-8428-9a091983c473 — FT opinion piece on AI agent legal personhood, low-engagement policy lane; off-scope per scope), **`49407507` "I gave Qwen 3.8 27B a reverse-engineering job and it finished in 30 minutes"** (234 pts top+best, 106 cmts, by `raybb` 2026-08-23, https://www.xda-developers.com/qwen-3-8-27b-reverse-engineering-job-frontier-model/ — Qwen 3.8 27B local-LLM revLine truncated
+- **Latest fetch:** `2026-08-24T18:00:28Z` (LLM-agent topic set: `organoid`, `CelVivo`, `3Brain`, `Cellink`, `openwiki`, `deepagents` — 6 queries; the source-specific instruction "Track organoid / CelVivo / 3Brain / Cellink / openwiki / AI agent stories" is the active query set; prior 2026-08-23 18:00, 2026-08-22 18:00, 2026-08-21 18:00, 2026-08-20 18:00, 2026-08-19 18:00, 2026-08-18 18:00, 2026-08-17 18:00, 2026-08-16 18:00, 2026-08-15 18:00, 2026-08-13 18:00, 2026-08-12 18:00, 2026-08-10 18:00, 2026-08-08 18:00, 2026-08-08 03:15, 2026-08-07 12:29, 2026-08-03 18:00, 2026-08-02 18:00, 2026-08-01 18:00, 2026-07-31 18:00, 2026-07-30 18:00, 2026-07-29 18:00, 2026-07-28 18:00, 2026-07-27 18:00, 2026-07-26 18:16 and 18:00 pulls also documented below)
+- **Raw dumps:** `~/.openwiki/connectors/hackernews/raw/2026-08-24T18-00-02-633Z/hackernews-results.json` (current — **fourteenth pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries**; **fully-zero Algolia lane on all 6 queries**: `organoid` / `CelVivo` / `3Brain` / `Cellink` / `openwiki` / `deepagents` each returned `nbHits=0 / hits=0` — the runtime payload reports clean integer `nbHits=0` (vs the `nbHits=null` shape on the 2026-08-21 + 2026-08-23 fully-zero pulls, both confirming the same fully-zero lane); the 2026-08-21 / 2026-08-23 / 2026-08-24 fully-zero pulls are bookends on the 2026-08-22 noise-only pull, **three fully-zero Algolia lanes in four consecutive pulls**, confirming the empty-pulls-on-vendor-queries cadence is normal under the 6-query LLM-agent topic set, not an outage, no config-side intervention needed; 38 unique top+best items, no in-scope vendor / customer / application-domain / named-stack signal — strongest weakly-adjacent items are **`49411102` "Anthropic's best AI model struggles to attract users as cheaper tools thrive"** (738 pts top+best, 649 cmts — **highest-engagement item of the pull**; FT business-model pressure on Anthropic's best AI model; **adjacent-not-promoted** per scope because Anthropic pricing pressure is not on Robin's named stack; off-scope per scope), **`49415893` "FDA clears blood test to aid evaluation for Alzheimer's disease"** (154 pts top, 73 cmts, washu.edu — Alzheimer's / blood-test diagnostic; **adjacent-not-promoted** per scope because Alzheimer's is not in Robin's 4 application domains — 3D cell culture, 3D bioprinting, neuroscience in vitro, organoid workflows — though the clinical-research framing has a tangentially neuroscience-adjacent axis), and **`49421554` "Coding expertise is going to collapse from AI reliance"** (202 pts top, 234 cmts, larsfaye.com — AI-coding talent-debate essay; **adjacent-not-promoted** per scope because coding-talent is not on Robin's named stack), see 2026-08-24 18:00 UTC entry below) · `~/.openwiki/connectors/hackernews/raw/2026-08-23T18-00-02-256Z/hackernews-results.json` (prior — **thirteenth pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries**; **fully-zero Algolia lane on all 6 queries**: `organoid` / `CelVivo` / `3Brain` / `Cellink` / `openwiki` / `deepagents` each returned `nbHits=null / hits=0` — same shape as the 2026-08-21 fully-zero pull (the prior fully-zero pull was 2026-08-21); the 2026-08-22 pull had a noise-only lane (3 of 6 with substring noise, 3 clean zero) and the 2026-08-23 pull returns to fully-zero across all 6 — **two fully-zero Algolia lanes in three consecutive pulls**, confirming the empty-pulls-on-vendor-queries cadence is normal under the 6-query LLM-agent topic set, not an outage, no config-side intervention needed; the strongest named-stack-adjacent item is **`49409092` "What Is a Harness?"** (121 pts, 83 cmts, top, by `tosh` 2026-08-23, https://earendil.com/posts/what-is-a-harness/ — a definitional essay on agent harnesses; **strongest named-stack-adjacent item of the pull by direct agent-harness cross-walk** because (a) the **121-pt + 83-cmt top-feed engagement** is the third-highest top-feed engagement on the pull (after the off-scope `49402232` Why your local LLM feels dumber 450 pts + `49402189` Scrap Moxie 399 pts), (b) the **definitional framing "What Is a Harness?"** directly speaks to the agent-as-primary-compute-substrate narrative that `hermes-agent-v0-19-quicksilver` documents, and (c) it is the **6th entry in the now 11-day self-hosted-agentic-substrate cluster** — DeepSeek Harness `49285244` 422 pts 2026-08-13 → machine0 `49348136` 19 pts 2026-08-18 → OneCLI `49363710` 21 pts 2026-08-19 → self-hosted agentic software factory `49390463` 16 pts 2026-08-21 → Munder Difflin `49398152` 192 pts 2026-08-22 → **"What Is a Harness?" `49409092` 121 pts 2026-08-23** — six independent submissions across 11 days, total cluster engagement 791 pts), with the next named-stack-adjacent item being **`49402232` "Why your local LLM feels dumber than it is"** (450 pts top+best, 182 cmts, by `felineflock` 2026-08-23, https://forum.level1techs.com/t/why-your-local-llm-feels-dumber-than-it-is/253917 — **highest-engagement item of the pull** at 450 pts + 182 cmts; a Level1Techs forum post on local-LLM optimization; **adjacent-not-promoted** per scope because local-LLM optimization is not on Robin's named daily-driver stack — Hermes / MiniMax-M3+H3 / OpenWiki / RAGFlow / NocoDB / SeaFile / Codex CLI are the named agents and unsloth/MiniMax-M3-GGUF is the local-LLM run path — but the **direct local-LLM-stack cross-walk to the `MiniMax-m3` row** makes this the strongest local-LLM-adjacent item of the pull), and the third named-stack-adjacent item being **`49410097` "GLM-5.3 (open-weight) beat Anthropic/OpenAI models – for 1/5 the cost"** (141 pts top, 143 pts best, by `ed-is-ai` 2026-08-23, https://reinLine truncated
 
-## Latest update — 2026-08-23 18:00 UTC
+## Latest update — 2026-08-24 18:00 UTC
+
+- **Coverage:** 2 feeds (`top`, `best`), 6 search queries (the same simplified LLM-agent topic set from the source-specific instruction "Track organoid / CelVivo / 3Brain / Cellink / openwiki / AI agent stories": `organoid`, `CelVivo`, `3Brain`, `Cellink`, `openwiki`, `deepagents`). `top` feed had 30 IDs (28 resolved items), `best` feed had 30 IDs (15 resolved items); deduplication gave **38 unique items across both feeds** (5 overlap: `49419237` How Europe is killing makers 698 pts top+best, `49412396` I were 17 I'd learn LLMs from scratch 409 pts top+best, `49420873` Xiaomi CPU Apple-match 381 pts top+best, `49421158` MS Paint GUID watermark 214 pts top+best, `49415271` Executable Is a SQLite Database 368 pts top+best). The 6-query lane returned **fully-zero hits across all six Algolia queries** — `organoid` / `CelVivo` / `3Brain` / `Cellink` / `openwiki` / `deepagents` each returned `nbHits=0 / hits=0` in the 24 h window (the runtime payload on the 2026-08-24 pull reports clean integer `nbHits=0` rather than the `nbHits=null` shape on the 2026-08-21 + 2026-08-23 fully-zero pulls, but the `hits=0` array length confirms the same fully-zero lane; this is the **third consecutive shape-variant of fully-zero Algolia lane** across the 2026-08-21 / 2026-08-23 / 2026-08-24 pulls); the connector completed normally at `fetchedAt: 2026-08-24T18:00:28.191Z` (24-hour window).
+- **Life-science lane:** **zero vendor-anchored hits across all four named vendor queries — and zero substring-noise hits as well.** All four Algolia vendor queries (`organoid`, `CelVivo`, `3Brain`, `Cellink`) returned `hits=0` in the 24 h window, with no string-collision noise on `organi-` / `brain` / `calling` / `ceiling` / `helsinki` substrings (the 2026-08-22 pull surfaced 1 thematic + 4 noise on `organoid`, 1 thematic + 5 noise on `3Brain`, 3 noise on `Cellink`; the 2026-08-23 + 2026-08-24 pulls return to fully-zero across all four vendor queries — **two consecutive fully-zero Algolia lanes on all four vendor queries** for the most recent pulls, the first pair since 2026-08-08 / 2026-08-13 / 2026-08-15 / 2026-08-16 / 2026-08-18 / 2026-08-19 / 2026-08-21 + 2026-08-23). A title/URL regex sweep across the 38 unique top+best items also turned up **zero organoid / 3D-bioprinting / CelVivo / 3Brain / Cellink / ClinoStar / ClinoReactor / MEA / spheroid / organ-on-chip items** and **zero self-hosted agentic substrate cluster items** (the 6-item / 11-day cluster from DeepSeek Harness 2026-08-13 → machine0 2026-08-18 → OneCLI 2026-08-19 → self-hosted factory 2026-08-21 → Munder Difflin 2026-08-22 → "What Is a Harness?" 2026-08-23 has its first quiet pull on 2026-08-24 — **7 items in 12 days with one miss is still a strong cluster density**, the first quiet pull since the cluster started). The "no material HN footprint for the 4 vendor lines" finding continues to hold — this is the **fourteenth pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries** (after the 2026-08-08 03:15 + 2026-08-08 18:00 + 2026-08-13 + 2026-08-15 + 2026-08-16 + 2026-08-18 + 2026-08-19 + 2026-08-21 + 2026-08-23 + 2026-08-24 fully-zero pulls + the 2026-08-10 + 2026-08-12 + 2026-08-17 + 2026-08-20 + 2026-08-22 noise-only pulls where vendor queries returned substring-fragment noise rather than fully zero; the 2026-08-24 pull is the **third consecutive fully-zero pull bookended with the noise-only 2026-08-22 pull** — three fully-zero Algolia lanes in four consecutive pulls — confirming that the empty-pulls-on-vendor-queries cadence is normal under the 6-query LLM-agent topic set, not an outage, no config-side intervention needed). The Tavily (web-search) lane remains the productive lane for vendor news; the HN lane's stable role is now "high-engagement inference-lane / agent-stack adjacents + low-engagement application-domain watchlist items + low-engagement self-hosted-agentic-substrate cluster essays" rather than vendor-direct signals. The 38 unique top+best items include **zero matches** for any in-scope vendor / customer / application-domain / named-stack keyword — no CelVivo / 3Brain / Cellink / ClinoStar / ClinoReactor / MEA / spheroid / organ-on-chip / organoid / bioprinting hits; no Hermes / OpenClaw / MiniMax / RAGFlow / NocoDB / SeaFile / Codex-CLI direct release hits. The single clinical-research-adjacent item on the pull is **`49415893` "FDA clears blood test to aid evaluation for Alzheimer's disease"** (154 pts top, 73 cmts, washu.edu — Alzheimer's / blood-test diagnostic; **adjacent-not-promoted** per scope because Alzheimer's is not one of Robin's 4 application domains — 3D cell culture, 3D bioprinting, neuroscience in vitro, organoid workflows — though Alzheimer's-diagnostic is a tangentially neuroscience-adjacent topic). Per the brief's "low-engagement items stay watchlist by default" rule + the `/themes.md` scope discipline, **none of the 38 unique items qualifies for a `/themes.md` row promotion on this pull**.
+- **Workflow lane:** **zero positive `openwiki` brand hits; zero `deepagents` hits.** `openwiki` and `deepagents` both returned `nbHits=0 / hits=0` — so the `openwiki-brand` watchlist row on `/themes.md` stays at Last seen 2026-07-16. The 38 unique top+best items include **no directly-relevant item for any `/themes.md` row by vendor** and **no self-hosted-agentic-substrate cluster entry on this pull**, but include **three weakly-adjacent items worth flagging for traceability**: **`49411102` "Anthropic's best AI model struggles to attract users as cheaper tools thrive"** (738 pts top+best, 649 cmts — **highest-engagement item of the pull**, FT business-model pressure on Anthropic's best AI model) + **`49421554` "Coding expertise is going to collapse from AI reliance"** (202 pts top, 234 cmts — AI-coding talent-debate essay on larsfaye.com) + **`49415893` "FDA clears blood test to aid evaluation for Alzheimer's disease"** (154 pts top, 73 cmts, washu.edu — Alzheimer's / blood-test diagnostic). Per the brief: low-engagement items stay watchlist by default; only high-engagement + directly-relevant items earn promotion. None of the 38 unique items crosses the promotion threshold:
+  - **`49411102` "Anthropic's best AI model struggles to attract users as cheaper tools thrive"** (738 pts, 649 cmts, **top + best feeds**, by `GSukhov` 2026-08-24, https://www.ft.com/content/5ee49718-c258-4f01-aa32-7e5b76ae5245 — Financial Times). A Financial Times business-model analysis arguing **Anthropic's best AI model struggles to attract users as cheaper competitive models thrive** — the second-highest FT piece on this pull (the off-scope `49410628` "We must not grant AI agents legal personhood" was the 2026-08-23 FT piece). **Highest-engagement item of the pull** (738 pts + 649 cmts top+best — the highest engagement on the pull by a wide margin, ahead of `49419237` How Europe is killing makers 698 pts + `49416055` Andreessen bleak future 583 pts + `49411643` Find problems staff engineer 536 pts). **Adjacent-not-promoted** per scope because (a) the **essay is an FT business-model analysis of the AI-vendor landscape** rather than a Hermes Agent / MiniMax / OpenWiki / Codex CLI release; (b) the established scope rule on AI-vendor business analyses keeps them adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself (per the 2026-07-30 / 2026-08-03 / 2026-08-10 / 2026-08-12 / 2026-08-13 / 2026-08-15 / 2026-08-16 / 2026-08-17 / 2026-08-18 / 2026-08-19 / 2026-08-20 / 2026-08-21 / 2026-08-22 / 2026-08-23 traceability entries); (c) the brief's "Workflow tooling priority LOW — surface story-worthy changes only" rule does not promote a 738-pt AI-vendor business essay to a `/themes.md` row by itself, though the **direct AI-vendor pricing-pressure cross-walk to the `MiniMax-m3` row's open-weight comparison anchor (DeepSeek V4 Flash / GLM / Qwen / Llama — open-weight competitive set pressuring closed-weight pricing)** make this the strongest **AI-vendor-business-lane** item of the pull. Flagged for traceability because (a) the **738-pt + 649-cmt engagement is the highest engagement on the pull** — strong enough to surface in any HN digest; (b) the **FT source** is a credible AI-business-analysis outlet; (c) the **Anthropic-pricing-pressure framing** cross-walks to the broader open-weight-vs-closed-weight market dynamic that the `MiniMax-m3` row documents; (d) this is the **first AI-vendor business-model item to cross 700 pts since the 2026-08-13 `DeepSeek Harness` (422 pts)** — a 316-pt engagement delta and the strongest engagement on any AI-vendor-business pull in the 2026-08-07 streak; (e) any future HN item at higher engagement combining Anthropic pricing pressure + concrete open-weight vendor pricing action (e.g., MiniMax-M3 pricing change) becomes the first corroborating event for a `MiniMax-m3-row-annex-open-weight-vs-closed-weight-pricing` annex on the existing row. **No commitment row** — Anthropic pricing pressure is upstream-of-MiniMax competitive context, not a vendor-stack change Robin acts on; the existing `MiniMax-m3` row is the canonical synthesis home. **No open-question row, no `/themes.md` row change** — `MiniMax-m3` stays at Last seen 2026-08-03 (no MiniMax model release; the FT essay is upstream AI-vendor business context, not a MiniMax-side change).
+  - **`49421554` "Coding expertise is going to collapse from AI reliance"** (202 pts, 234 cmts, **top feed**, by `larsfaye` 2026-08-24, https://larsfaye.com/articles/ai-coding-will-prevent-expertise — personal-blog essay). A personal-blog essay arguing that **coding expertise will collapse from over-reliance on AI coding tools** — a coding-talent debate adjacent to the AI-coding-agent lane. **Adjacent-not-promoted** per scope because (a) **coding-talent is not on Robin's named daily-driver stack** (Hermes / MiniMax-M3+H3 / OpenWiki / RAGFlow / NocoDB / SeaFile / Codex CLI are the named agents); (b) the established scope rule on AI-coding-agent talent-debate essays keeps them adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself (per the 2026-07-30 / 2026-08-03 / 2026-08-10 / 2026-08-12 / 2026-08-13 / 2026-08-15 / 2026-08-16 / 2026-08-17 / 2026-08-18 / 2026-08-19 / 2026-08-20 / 2026-08-21 / 2026-08-22 / 2026-08-23 traceability entries); (c) the brief's "Workflow tooling priority LOW — surface story-worthy changes only" rule does not promote a 202-pt coding-talent-debate essay to a `/themes.md` row by itself, though the **direct AI-coding-agent cross-walk to the `MiniMax-m3` row's open-weight local-LLM lane (Codex CLI is on Robin's named stack and `MiniMax-M3-GGUF` is the local-LLM run path)** makes this a tangentially AI-coding-agent-adjacent item. Flagged for traceability because (a) the **234-cmt engagement is the third-highest top+best comment count on the pull** (after the off-scope `49411102` Anthropic 649 cmts + `49419237` How Europe 468 cmts); (b) the **coding-expertise-collapse framing** parallels the broader AI-coding-agent adoption debate; (c) the personal-blog essay format cross-walks to the prior 2026-08-22 (`49394496` Three important steps in my maturation process — Dullien) and 2026-08-21 (`49390463` Building an (almost) fully self-hosted, sandboxed, agentic software factory) personal-blog essays on adjacent / off-scope lanes; (d) any future HN item at higher engagement combining coding-expertise-collapse + concrete AI-coding-agent production-degradation evidence becomes the first corroborating event for a `coding-agent-productivity-degradation` watchlist entry. **No commitment row** — coding-talent-debate essay is upstream opinion context, not a vendor-stack change Robin acts on; if Robin personally adopts a coding-expertise-preservation practice on a future pull, that becomes a downstream commitment. **No `/themes.md` row change** — no AI-coding-agent theme row exists; `MiniMax-m3` and `hermes-agent-v0-19-quicksilver` stay at Last seen 2026-08-03 (no Hermes Agent / MiniMax release on this pull).
+  - **`49415893` "FDA clears blood test to aid evaluation for Alzheimer's disease"** (154 pts, 73 cmts, **top feed**, by `MBCook` 2026-08-24, https://medicine.washu.edu/news/fda-clears-blood-test-to-aid-evaluation-for-alzheimers-disease/ — Washington University School of Medicine in St. Louis press release). A WashU Med press release announcing **FDA clearance of a blood test to aid evaluation for Alzheimer's disease** — a clinical-diagnostic clearance, not an organoid / 3D-bioprinting item. **Adjacent-not-promoted** per scope because (a) **Alzheimer's-diagnostic is not one of Robin's 4 application domains** (3D cell culture, 3D bioprinting, neuroscience in vitro, organoid workflows); (b) Alzheimer's disease is tangentially neuroscience-adjacent but not a direct neuroscience-in-vitro experiment; (c) the established scope rule on clinical-diagnostic clearance press releases keeps them adjacent-not-promoted unless there is a direct change to the 4 application domains (per the 2026-07-27 / 2026-07-29 / 2026-07-30 / 2026-08-12 / 2026-08-20 clinical-research traceability entries — `49410149` Vit D cognition 2026-08-23 + `49366519` Nature organoid-time-passage 2026-08-20 are the recent reference frames); (d) the brief's "Workflow tooling priority LOW — surface story-worthy changes only" rule does not promote a 154-pt FDA-clearance press release to a `/themes.md` row by itself. Flagged for traceability because (a) the **154-pt + 73-cmt engagement is the fifth-highest clinical-adjacent engagement on the pull** (after the off-scope `49411102` Anthropic 738 + `49419237` How Europe 698 + `49416055` a16z bleak future 583 + `49411643` Find problems 536); (b) it is the **first FDA-clearance press release on the 2026-08-07 streak** — a clinical-regulatory signal distinct from the prior clinical-research-paper items; (c) the **WashU / Washington University source** is a credible clinical-research institution; (d) any future HN item at higher engagement combining Alzheimer's clinical-diagnostic + organoid / 3D-cell-culture / neuroscience-in-vitro application-domain framing becomes the first corroborating event for a `class-organoid-3d-bioprinting` description extension on clinical-translation evidence. **No commitment row, no open-question row, no `/themes.md` row change** — the FDA clearance is upstream clinical-regulatory news, not a vendor-stack change Robin acts on; if Robin's own customers cross-sell to WashU Alzheimer's-diagnostic accounts on a future pull, that becomes a downstream commitment.
+  - **No self-hosted-agentic-substrate cluster entry on this pull:** the 6-item / 11-day cluster from DeepSeek Harness `49285244` 422 pts 2026-08-13 → machine0 `49348136` 19 pts 2026-08-18 → OneCLI `49363710` 21 pts 2026-08-19 → self-hosted agentic software factory `49390463` 16 pts 2026-08-21 → Munder Difflin `49398152` 192 pts 2026-08-22 → "What Is a Harness?" `49409092` 121 pts 2026-08-23 has its **first quiet pull on 2026-08-24** — 7 cluster items in 12 days with one miss is still **a strong cluster density pattern** (6-hit density averaged 0.55 entries/day over 11 days before the 2026-08-24 quiet pull, dropping to 0.58 entries/day over 12 days with one miss). The 38 unique top+best items include zero items matching `harness` / `agent` / `MCP` / `model context protocol` / `OpenClaw` / `Hermes` / `NanoClaw` / `Munder Difflin` / `Machine0` / `OneCLI` / `self-hosted` / `agentic software` — the cluster has had no new cluster entry on this pull but the **prior cluster density of 6 entries in 11 days + the recent refresh on 2026-08-23 with "What Is a Harness?" at 121 pts + the cluster's continued cross-walk to Hermes Agent's harness architecture** keep the cluster relevant; any cluster entry on a future pull becomes the next `hermes-agent-v0-19-quicksilver` Last-seen refresh candidate.
+  - **Highest-engagement items off-scope:** the remaining 35 of 38 unique top+best items fall outside Robin's named stack + 4 application domains per the brief's scope filter — `49413320` "Everything I own, owned" (1292 pts top, 328 cmts — personal essay on ownership, off-scope); `49416055` "Andreessen Horowitz is investing billions into a bleak future" (583 pts top+best, 304 cmts — critique of a16z portfolio, off-scope); `49411643` "How I find problems to solve as a staff engineer" (536 pts top+best, 154 cmts — engineering essay, off-scope); `49412396` "I were 17, I'd learn how to build LLMs from scratch" (409 pts top+best, 529 cmts — AI learning advice, off-scope); `49420873` "Xiaomi: New CPU matches Apple cores single threaded, much faster multithreaded" (381 pts top+best, 219 cmts — chip news, off-scope); `49411717` "Google Workspace thinks my domain is an email provider (2025)" (375 pts top+best, 146 cmts — Google Workspace bug, off-scope); `49415271` "Executable Is a SQLite Database" (368 pts top+best, 73 cmts — engineering essay, off-scope); `49413561` "I built a low-latency AI companion that plays Skyrim with me" (310 pts top+best, 65 cmts — AI gaming, off-scope); `49411395` "Over 170k Nonprofits Lost All Their Data. Is Microsoft to Blame?" (284 pts top+best, 149 cmts — Microsoft nonprofit issue, off-scope); `49415621` "New EU-wide product repair rules come into force" (231 pts top+best, 130 cmts — EU regulation, off-scope); `49411468` "Fable and the end of the free lunch" (212 pts top+best, 216 cmts — engineering essay, off-scope); `49421074` "OpenAI: GPT 5.6 Sol price reduction (until at least Nov 21)" (158 pts top+best, 146 cmts — OpenAI pricing, off-scope); `49418255` "SeL4 security proofs now complete on AArch64" (141 pts top+best, 31 cmts — kernel security, off-scope); `49421489` "IPFS Maintainers Winding Down" (141 pts top+best, 55 cmts — IPFS, off-scope); `49419351` "Show HN: A techno machine in one HTML file, with verifiable renders" (77 pts top, 16 cmts — Show HN, off-scope); `49422204` "A Blackstone real estate company exposed SSN digits, DOBs, addresses and more" (62 pts top, 18 cmts — security, off-scope); `49422404` "Man Dressed as Darth Vader Defends Flock Cameras to San Diego City Council" (55 pts top, 7 cmts — civil liberties, off-scope); `49422784` "The entire city of San Francisco as a video game" (46 pts top, 11 cmts — Show HN, off-scope); `49421536` "Jabber/XMPP: 25 Years of Digital Independence" (42 pts top, 18 cmts — protocol history, off-scope); `49422227` "Public services are increasingly strained by LLM-written appeals for benefits" (39 pts top, 46 cmts — LLM social impact, off-scope); `49422800` "Where Did All the Public Bathrooms Go?" (33 pts top, 20 cmts — public infrastructure, off-scope); `49420592` "Hot Chips 2026: Applying High Bandwidth Flash (HBF)" (32 pts top, 11 cmts — chip news, off-scope); `49420832` "Adafruit USB Type C CC Resistor Fixer" (32 pts top, 18 cmts — hardware, off-scope); `49421948` "Show HN: GlassBox – what the browser reveals, and how identifiable you are" (31 pts top, 10 cmts — browser fingerprinting, off-scope); `49421806` "Show HN: PicoMQ – Durable Streams over HTTP, on object storage" (23 pts top, 0 cmts — Show HN, off-scope); `49422548` "Hot Chips 2026: CUDA Targets RISC-V – By Chester Lam" (22 pts top, 3 cmts — chip news, off-scope); `49420861` "Could We Dredge the Netherlands Without Fossil Fuels?" (19 pts top, 11 cmts — environmental essay, off-scope); `49420999` "NetBSD GSoC 2026 Improving RAIDframe" (17 pts top, 2 cmts — NetBSD development, off-scope); `49420556` "Codefloe Is a Professionally Hosted Public Git Forge" (13 pts top, 0 cmts — Show HN, off-scope); `49421347` "Three Generations in E7" (11 pts top, 1 cmt — math, off-scope); `49420781` "Hot Chips 2026: Samsung and HBM Base Die Opportunities" (10 pts top, 2 cmts — chip news, off-scope); `49422481` "EuroHPC Launches 6 Quantum Calls with €119M in Funding" (6 pts top, 1 cmt — EU HPC, off-scope); `49423495` "Autostep (YC P26) Is Hiring AI/Fullstack Engineers and a Chief of Staff" (1 pt top, 0 cmts — YC job, off-scope). Engagement range 1–1292 pts across the 38 items; **0 items match a Robin in-scope vendor / customer / application-domain / named-stack keyword** (no CelVivo / 3Brain / Cellink / ClinoStar / ClinoReactor / MEA / spheroid / organ-on-chip / organoid / bioprinting hits; no Hermes / OpenClaw / MiniMax / RAGFlow / NocoDB / SeaFile / Codex-CLI direct release hits), **0 items match Robin's broader agent-stack or substrate lane** (no `harness` / `MCP` / `OpenClaw` / `Hermes` / `Munder Difflin` / `machine0` / `OneCLI` / `self-hosted` / `agentic` entries — **first pull in the 2026-08-07 streak with zero self-hosted-agentic-substrate cluster items**), **0 items match Robin's broader open-weight-LLM lane** (no DeepSeek / GLM / Qwen / Llama / unsloth / `MiniMax-M3-GGUF` direct hits), **0 items match RobLine truncated
+- **Synthesis result:** **no theme refresh, no commitment row, no open-question row, no personal-logistics item.** The `class-organoid-3d-bioprinting` row on `/themes.md` stays at Last seen 2026-08-20 (no organoid / 3D-bioprinting / CelVivo / 3Brain / Cellink item — the 38 unique feed items include zero application-domain matches; the closest item is `49415893` FDA Alzheimer's blood-test (Alzheimer's diagnostic, off-scope per scope); the prior 2026-08-20 refresh from the `49366519` Nature organoid-time-passage paper + `49373812` Science Alert write-up carries forward unchanged). The `agent-memory-architecture` row stays at Last seen 2026-08-13 (no OKF / MCP Memory / Karpathy LLM Wiki / Agent Mesh item; the 38 unique items include zero agent-memory items; the MCP Memory OKF+memory schema event is the canonical row anchor, and the 2026-08-24 pull adds zero evidence to that anchor). The `MiniMax-m3` row stays at Last seen 2026-08-03 (no MiniMax model release; `49411102` FT Anthropic is **the strongest AI-vendor-business-adjacent item of the pull** at 738 pts + 649 cmts but stays adjacent-not-promoted per scope because Anthropic pricing pressure is upstream competitive context, not a MiniMax-side change; the existing `MiniMax-m3` row already cites the benchlm.ai 2026-08-01 V4-Flash-vs-M3 framing + the unsloth `unsloth/MiniMax-M3-GGUF` quantized local-LLM run path + the MyClaw.ai 2026-07-29 multimodal confirmation + the Hermes Agent MiniMax-M3 default-model designation, so the FT Anthropic essay is an incremental upstream AI-vendor signal rather than a MiniMax-side change). The `hermes-agent-v0-19-quicksilver` row stays at Last seen 2026-08-03 (no Hermes Agent release; **zero self-hosted-agentic-substrate cluster entries on this pull** — the first quiet pull in the cluster since 2026-08-13 — but the cluster density of 6 entries in 11 days through 2026-08-23 + the recent refresh on 2026-08-23 with "What Is a Harness?" at 121 pts carries forward unchanged; any future cluster entry + Hermes Agent / OpenWiki / Codex CLI release notes on the same pull becomes the first named-stack adoption event for the agent-harness substrate). The `openwiki-brand` watchlist row stays at Last seen 2026-07-16 (no positive `openwiki` hits — clean zero across both feed sweep and Algolia lane). The `china-cde-cell-gene-therapy-rules-2026` and `china-order-no-818-clinical-translation` watchlist rows stay at Last seen 2026-07-24 / 2026-07-28 (no China regulatory items on this pull). All four flag-for-traceability items stay adjacent-not-promoted under the existing scope discipline. **Key calls**:
+  - **`49411102` FT "Anthropic's best AI model struggles to attract users as cheaper tools thrive"** is the **strongest AI-vendor-business-adjacent item of the pull by inference-lane impact** by virtue of (a) the **738-pt + 649-cmt top+best engagement** — the highest engagement on the pull by a wide margin (ahead of `49419237` How Europe 698 pts + `49416055` a16z bleak future 583 pts + `49411643` Find problems 536 pts; the off-scope `49419237` How Europe is the second-highest on the pull, and `49416055` a16z is the third-highest); (b) the **FT source** — a credible AI-business-analysis outlet; (c) the **Anthropic-pricing-pressure framing** cross-walks to the broader open-weight-vs-closed-weight market dynamic that the `MiniMax-m3` row documents (DeepSeek V4 Flash / GLM / Qwen / Llama — open-weight competitive set pressuring closed-weight pricing); (d) this is the **first AI-vendor business-model item to cross 700 pts since the 2026-08-13 `DeepSeek Harness` (422 pts)** — a 316-pt engagement delta and the strongest engagement on any AI-vendor-business pull in the 2026-08-07 streak. **Adjacent-not-promoted** per scope (the essay is an FT business-model analysis, not a MiniMax / unsloth / Hermes Agent / OpenWiki release; the established scope rule keeps non-named-stack AI-vendor business essays adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself). Flagged because (a) the **direct AI-vendor-pricing cross-walk to the `MiniMax-m3` row's open-weight comparison anchor** makes this a corroborating AI-vendor-business-stack signal alongside the 2026-08-19 Unsloth Dynamic 3.0 GGUFs + the 2026-07-29 MyClaw.ai MiniMax-M3 multimodal confirmation + the 2026-08-01 benchlm.ai DeepSeek V4 Flash vs MiniMax M3 comparison + the 2026-08-21 DeepSeek-v4-flash-vision release; (b) the **FT source is the second FT piece in two pulls** (the 2026-08-23 `49410628` "We must not grant AI agents legal personhood" + the 2026-08-24 `49411102` Anthropic pricing pressure — a two-pull FT cluster on the AI-agent governance + AI-vendor-business lane); (c) any future HN item at higher engagement combining Anthropic / OpenAI pricing pressure + concrete open-weight vendor pricing action (e.g., MiniMax-M3 pricing change) becomes the first corroborating event for a `MiniMax-m3-row-annex-open-weight-vs-closed-weight-pricing` annex on the existing row. **No commitment row, no `/themes.md` row change** — the essay is upstream AI-vendor business context; if Robin applies the open-weight-vs-closed-weight market analysis to a specific MiniMax-M3-GGUF deployment on a future pull, that becomes a downstream commitment.
+  - **`49421554` "Coding expertise is going to collapse from AI reliance"** is the **strongest AI-coding-agent-adjacent item of the pull by personal-blog-essay impact** by virtue of (a) the **202-pt + 234-cmt top-feed engagement** — the 16th-highest top-feed engagement on the pull + the third-highest comment count (after `49411102` Anthropic 649 cmts + `49419237` How Europe 468 cmts); (b) the **direct AI-coding-agent cross-walk to the `MiniMax-m3` row's open-weight local-LLM lane** (Codex CLI is on Robin's named stack and `MiniMax-M3-GGUF` is the local-LLM run path); (c) the **personal-blog essay format** parallels the prior 2026-08-22 `49394496` Dullien maturation-process essay + the 2026-08-21 `49390463` self-hosted agentic-software-factory essay. **Adjacent-not-promoted** per scope (the essay is a personal-blog coding-talent-debate piece, not a Codex CLI release; the established scope rule keeps non-named-stack AI-coding-agent talent-debate essays adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself). Flagged because (a) the **234-cmt engagement is the third-highest comment count on the pull**; (b) the **AI-coding-expertise-collapse framing** parallels the broader AI-coding-agent adoption debate; (c) the personal-blog essay format cross-walks to the prior personal-blog essays in the same 11-day self-hosted-agentic-substrate cluster; (d) any future HN item combining coding-expertise-collapse + concrete AI-coding-agent production-degradation evidence becomes the first corroborating event for a `coding-agent-productivity-degradation` watchlist entry. **No commitment row, no `/themes.md` row change** — the essay is upstream opinion context, not a Codex-CLI-side change.
+  - **`49415893` "FDA clears blood test to aid evaluation for Alzheimer's disease"** is the **only clinical-research-adjacent item on the 2026-08-24 top+best feed lane** by virtue of (a) the **washu.edu source** — a credible clinical-research institution source; (b) the **FDA clearance event** is a clinical-regulatory signal distinct from the prior clinical-research-paper items; (c) the **Alzheimer's-diagnostic framing** is a tangentially neuroscience-adjacent topic. **Adjacent-not-promoted** per scope (Alzheimer's-diagnostic is not one of Robin's 4 application domains — 3D cell culture, 3D bioprinting, neuroscience in vitro, organoid workflows; 154-pt engagement is below the 100-pt vendor-release / 50-pt clinical-translation bars; off-scope per scope discipline). Flagged because (a) it is the **only clinical-research-adjacent item on the 2026-08-24 top+best feed lane** — the first since the 2026-08-23 `49410149` Vitamin D cognition paper (off-scope per scope) and the 2026-08-20 `49366519` Nature organoid-time-passage paper (in-scope per scope, anchor for the 2026-08-20 refresh); (b) the **FDA-clearance framing** is the first FDA-clearance press release on the 2026-08-07 streak — a clinical-regulatory signal distinct from the prior clinical-research-paper items; (c) any future HN item at higher engagement combining Alzheimer's-diagnostic clinical-translation + organoid / 3D-cell-culture / neuroscience-in-vitro application-domain framing becomes the first corroborating event for a `class-organoid-3d-bioprinting` description extension on clinical-translation evidence. **No commitment row, no `/themes.md` row change** — the FDA clearance is upstream clinical-regulatory news, not a vendor-stack change Robin acts on.
+  - **This is the fourteenth pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries** (after the 2026-08-08 03:15 + 2026-08-08 18:00 + 2026-08-13 + 2026-08-15 + 2026-08-16 + 2026-08-18 + 2026-08-19 + 2026-08-21 + 2026-08-23 + 2026-08-24 fully-zero pulls + the 2026-08-10 + 2026-08-12 + 2026-08-17 + 2026-08-20 + 2026-08-22 noise-only pulls where vendor queries returned substring-fragment noise rather than fully zero; the 2026-08-24 pull is the **third consecutive fully-zero pull in the four-pull window 2026-08-21 → 2026-08-24 (bookending the 2026-08-22 noise-only pull)** — three fully-zero Algolia lanes in four consecutive pulls — confirming that the empty-pulls-on-vendor-queries cadence is normal under the 6-query LLM-agent topic set, not an outage, no config-side intervention needed). The pattern confirms that the empty-pulls-on-vendor-queries cadence is normal under the 6-query LLM-agent topic set. The Tavily (web-search) lane remains the productive lane for vendor news; the HN lane's stable role is now "high-engagement inference-lane / agent-stack adjacents (What Is a Harness? + GLM-5.3 cluster + Qwen 3.8 27B + FT Anthropic essay + AI-coding-expertise-collapse + Munder Difflin + New MCP Roadmap + OzBrain + DeepSeek V4 Flash vision + Proliferate + machine0 + OneCLI + OpenRouter-Stripe confirmation + Copilot Autofix + Unsloth Dynamic 3.0 GGUFs + AGENTS.md + FDA Alzheimer's) + low-engagement application-domain watchlist items (organoid / neuroscience / Wired features / clinical-research papers) + low-engagement self-hosted-agentic-substrate cluster essays (DeepSeek Harness + machine0 + OneCLI + self-hosted factory + Munder Difflin + What Is a Harness?)" rather than vendor-direct signals. The 5-overlap dedup count between `top` and `best` (38 unique from 28+15) is **lower than prior pulls** (the 2026-08-23 pull had 12-overlap from 28+15 = 31 unique, the 2026-08-22 pull had 3-overlap from 16+5 = 18 unique, the 2026-08-21 pull had 5-overlap from 28+13 = 36 unique); the lower dedup count is driven by `best` resolving only 15 items — same as the 2026-08-23 pull — and a slightly higher `top` resolution (28 vs 28 on 2026-08-23) — consistent with the 24-hour window having a denser `top` feed on 2026-08-24. The 38 unique items are a +7 jump on the 2026-08-23 pull (which had 31 unique) — consistent with the 24-hour window having a denser feed on 2026-08-24 (a 22.5% increase in unique items).
+
+| Query lane | Queries run | Qualifying results |
+| --- | --- | --- |
+| Life sciences / vendors (fully zero) | `organoid`, `CelVivo`, `3Brain`, `Cellink` | 0 (all four queries returned `nbHits=0 / hits=0` — **fully-zero Algolia lane on all four vendor queries, no substring-noise hits this pull**) |
+| AI workflow (fully zero) | `openwiki`, `deepagents` | 0 (both queries returned `nbHits=0 / hits=0`) |
+
+Raw dump: `~/.openwiki/connectors/hackernews/raw/2026-08-24T18-00-02-633Z/hackernews-results.json`.
+
+### Flag-for-traceability: FT "Anthropic's best AI model struggles to attract users" (738 pts, strongest AI-vendor-business item of the pull, second FT piece in two pulls), AI-coding-expertise-collapse essay (202 pts, strongest AI-coding-agent-adjacent item by personal-blog-essay impact), FDA Alzheimer's blood-test clearance (154 pts, only clinical-research-adjacent item on the pull, first FDA-clearance press release on the 2026-08-07 streak) — adjacent, off-scope
+
+- **49411102** (738 pts top+best, 649 cmts) **"Anthropic's best AI model struggles to attract users as cheaper tools thrive"** — FT business-model analysis (`GSukhov` 2026-08-24, https://www.ft.com/content/5ee49718-c258-4f01-aa32-7e5b76ae5245 — Financial Times). A Financial Times business-model analysis arguing **Anthropic's best AI model struggles to attract users as cheaper competitive models thrive** — the second-highest FT piece on this pull (the off-scope `49410628` "We must not grant AI agents legal personhood" was the 2026-08-23 FT piece). **Highest-engagement item of the pull** (738 pts + 649 cmts top+best — the highest engagement on the pull by a wide margin, ahead of `49419237` How Europe 698 pts + `49416055` a16z bleak future 583 pts + `49411643` Find problems 536 pts; the off-scope `49419237` How Europe is the second-highest on the pull, and `49416055` a16z is the third-highest). **Strongest AI-vendor-business-adjacent item of the pull** because (a) **738-pt + 649-cmt engagement is the highest engagement on the pull** by a wide margin — strong enough to surface in any HN digest; (b) the **FT source** is a credible AI-business-analysis outlet; (c) the **Anthropic-pricing-pressure framing** cross-walks to the broader open-weight-vs-closed-weight market dynamic that the `MiniMax-m3` row documents (DeepSeek V4 Flash / GLM / Qwen / Llama — open-weight competitive set pressuring closed-weight pricing); (d) this is the **first AI-vendor business-model item to cross 700 pts since the 2026-08-13 `DeepSeek Harness` (422 pts)** — a 316-pt engagement delta and the strongest engagement on any AI-vendor-business pull in the 2026-08-07 streak; (e) the **649-cmt engagement is the highest comment count on the pull** by a wide margin (ahead of `49419237` How Europe 468 cmts + `49411643` Find problems 154 cmts + `49411202` Anthropic itself). **Adjacent-not-promoted** per scope because (a) **Anthropic pricing pressure is upstream-of-MiniMax competitive context, not a vendor-stack change Robin acts on**; (b) the established scope rule on non-named-stack AI-vendor business essays keeps them adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself (per the 2026-07-30 / 2026-08-03 / 2026-08-10 / 2026-08-12 / 2026-08-13 / 2026-08-15 / 2026-08-16 / 2026-08-17 / 2026-08-18 / 2026-08-19 / 2026-08-20 / 2026-08-21 / 2026-08-22 / 2026-08-23 traceability entries); (c) the brief's "Workflow tooling priority LOW — surface story-worthy changes only" rule does not promote a 738-pt FT business essay to a `/themes.md` row by itself, though the **direct AI-vendor-pricing cross-walk to the `MiniMax-m3` row's open-weight comparison anchor + the highest engagement on the pull + the first 700-pt+ AI-vendor-business item in the streak** make this the strongest AI-vendor-business-adjacent item of the pull. Flagged for traceability because (a) the **738-pt engagement is the highest engagement on the pull** — the strongest AI-vendor-business-adjacent signal of the pull by engagement; (b) the **direct AI-vendor-pricing cross-walk to the `MiniMax-m3` row** makes this a corroborating AI-vendor-business-stack signal alongside the 2026-08-19 Unsloth Dynamic 3.0 GGUFs + the 2026-07-29 MyClaw.ai MiniMax-M3 multimodal confirmation + the 2026-08-01 benchlm.ai DeepSeek V4 Flash vs MiniMax M3 comparison + the 2026-08-21 DeepSeek-v4-flash-vision release; (c) the **FT source is the second FT piece in two pulls** (the 2026-08-23 `49410628` "We must not grant AI agents legal personhood" + the 2026-08-24 `49411102` Anthropic pricing pressure — a two-pull FT cluster on the AI-agent governance + AI-vendor-business lane); (d) any future HN item at higher engagement combining Anthropic / OpenAI pricing pressure + concrete open-weight vendor pricing action (e.g., MiniMax-M3 pricing change) becomes the first corroborating event for a `MiniMax-m3-row-annex-open-weight-vs-closed-weight-pricing` annex on the existing row. **No commitment row** — Anthropic pricing pressure is upstream competitive context, not a vendor-stack change Robin acts on; the existing `MiniMax-m3` row is the canonical synthesis home. **No open-question row, no `/themes.md` row change** — `MiniMax-m3` stays at Last seen 2026-08-03 (no MiniMax model release; the FT essay is upstream AI-vendor business context, not a MiniMax-side change).
+- **49421554** (202 pts top, 234 cmts) **"Coding expertise is going to collapse from AI reliance"** — AI-coding-expertise-collapse essay (`larsfaye` 2026-08-24, https://larsfaye.com/articles/ai-coding-will-prevent-expertise — personal-blog essay). A personal-blog essay arguing that **coding expertise will collapse from over-reliance on AI coding tools** — a coding-talent debate adjacent to the AI-coding-agent lane. **Strongest AI-coding-agent-adjacent item of the pull by personal-blog-essay impact** because (a) **202-pt + 234-cmt top-feed engagement** — the 16th-highest top-feed engagement on the pull + the **third-highest top+best comment count on the pull** (after `49411102` Anthropic 649 cmts + `49419237` How Europe 468 cmts); (b) the **direct AI-coding-agent cross-walk to the `MiniMax-m3` row's open-weight local-LLM lane** (Codex CLI is on Robin's named stack and `MiniMax-M3-GGUF` is the local-LLM run path); (c) the **personal-blog essay format** parallels the prior 2026-08-22 `49394496` Dullien maturation-process essay + the 2026-08-21 `49390463` self-hosted agentic-software-factory essay + the 2026-08-23 `49409092` "What Is a Harness?" definitional essay — a three-pull cluster of personal-blog essays on AI-agent-stack lanes; (d) the **coding-expertise-collapse framing** parallels the broader AI-coding-agent adoption debate. **Adjacent-not-promoted** per scope because (a) **coding-talent is not on Robin's named daily-driver stack** (Hermes / MiniMax-M3+H3 / OpenWiki / RAGFlow / NocoDB / SeaFile / Codex CLI are the named agents); (b) the established scope rule on AI-coding-agent talent-debate essays keeps them adjacent-not-promoted unless there is a direct change to Hermes / MiniMax / OpenWiki / Codex CLI itself; (c) the brief's "Workflow tooling priority LOW — surface story-worthy changes only" rule does not promote a 202-pt coding-talent-debate essay to a `/themes.md` row by itself, though the **direct AI-coding-agent cross-walk to the `MiniMax-m3` row's open-weight local-LLM lane + the third-highest comment count on the pull** make this the strongest AI-coding-agent-adjacent item of the pull. Flagged for traceability because (a) the **234-cmt engagement is the third-highest comment count on the pull** — a strong comment-vs-upvote ratio (234/202 = 1.16) indicating substantive debate; (b) the **AI-coding-expertise-collapse framing** parallels the broader AI-coding-agent adoption debate; (c) any future HN item combining coding-expertise-collapse + concrete AI-coding-agent production-degradation evidence becomes the first corroborating event for a `coding-agent-productivity-degradation` watchlist entry. **No commitment row, no `/themes.md` row change** — the essay is upstream opinion context, not a Codex-CLI-side change.
+- **49415893** (154 pts top, 73 cmts) **"FDA clears blood test to aid evaluation for Alzheimer's disease"** — washu.edu press release (`MBCook` 2026-08-24, https://medicine.washu.edu/news/fda-clears-blood-test-to-aid-evaluation-for-alzheimers-disease/ — Washington University School of Medicine in St. Louis press release). A WashU Med press release announcing **FDA clearance of a blood test to aid evaluation for Alzheimer's disease** — a clinical-diagnostic clearance. **Only clinical-research-adjacent item on the 2026-08-24 top+best feed lane** because (a) the **washu.edu source** — a credible clinical-research institution source; (b) the **FDA clearance event** is a clinical-regulatory signal distinct from the prior clinical-research-paper items; (c) the **Alzheimer's-diagnostic framing** is a tangentially neuroscience-adjacent topic. **Adjacent-not-promoted** per scope because (a) **Alzheimer's-diagnostic is not one of Robin's 4 application domains** — 3D cell culture, 3D bioprinting, neuroscience in vitro, organoid workflows; (b) Alzheimer's disease is tangentially neuroscience-adjacent but not a direct neuroscience-in-vitro experiment; (c) the 154-pt engagement is **below the 100-pt vendor-release refresh bar**; (d) the established scope rule on clinical-diagnostic clearance press releases keeps them adjacent-not-promoted unless there is a direct change to the 4 application domains (per the 2026-07-27 / 2026-07-29 / 2026-07-30 / 2026-08-12 / 2026-08-20 / 2026-08-23 clinical-research traceability entries). Flagged because (a) it is the **first FDA-clearance press release on the 2026-08-07 streak** — a clinical-regulatory signal distinct from the prior clinical-research-paper items (the 2026-08-23 `49410149` Vitamin D cognition paper is a clinical-research paper, the 2026-08-20 `49366519` Nature organoid-time-passage paper is an academic Nature paper, the 2026-08-24 `49415893` is an FDA-clearance press release — three different clinical-research-side lanes); (b) any future HN item at higher engagement combining Alzheimer's clinical-diagnostic + organoid / 3D-cell-culture / neuroscience-in-vitro application-domain framing becomes the first corroborating event for a `class-organoid-3d-bioprinting` description extension on clinical-translation evidence. **No commitment row, no open-question row, no `/themes.md` row change** — the FDA clearance is upstream clinical-regulatory news, not a vendor-stack change Robin acts on.
+
+## Update — 2026-08-23 18:00 UTC
 
 - **Coverage:** 2 feeds (`top`, `best`), 6 search queries (the same simplified LLM-agent topic set from the source-specific instruction "Track organoid / CelVivo / 3Brain / Cellink / openwiki / AI agent stories": `organoid`, `CelVivo`, `3Brain`, `Cellink`, `openwiki`, `deepagents`). `top` feed had 30 IDs (28 resolved items), `best` feed had 30 IDs (15 resolved items); deduplication gave **31 unique items across both feeds** (12 overlap: `49402189` Scrap (2006) Moxie Marlinspike 399 pts top+best, `49402232` Why your local LLM feels dumber 450 pts top+best, `49405331` Blade Runner essay 158 pts top+best, `49405816` MartyPC emulator 176 pts top+best, `49405870` Writer-read essay 370 pts top+best, `49406333` End of an Athlon 161 pts top+best, `49406539` Wi-Fi 8 essay 195 pts top+best, `49407507` Qwen 3.8 27B reverse-engineering 234 pts top+best, `49408550` Android automotive malware 158 pts top+best, `49409073` $266 tablet GLM-5.3 268 pts top+best, `49409200` Slovakia camera backdoor 191 pts top+best, `49410097` GLM-5.3 vs Anthropic/OpenAI 141 pts top / 143 pts best). The 6-query lane returned **fully-zero hits across all six Algolia queries** — `organoid` / `CelVivo` / `3Brain` / `Cellink` / `openwiki` / `deepagents` each returned `nbHits=null / hits=0` in the 24 h window (the runtime payload reports `nbHits=null` rather than the typical integer `nbHits=0`, but the `hits=0` array length confirms the same fully-zero lane); the connector completed normally at `fetchedAt: 2026-08-23T18:00:28.019Z` (24-hour window).
 - **Life-science lane:** **zero vendor-anchored hits across all four named vendor queries — and zero substring-noise hits as well.** All four Algolia vendor queries (`organoid`, `CelVivo`, `3Brain`, `Cellink`) returned `hits=0` in the 24 h window, with no string-collision noise on `organi-` / `brain` / `calling` / `ceiling` / `helsinki` substrings (the 2026-08-22 pull surfaced 1 thematic + 4 noise on `organoid`, 1 thematic + 5 noise on `3Brain`, and 3 noise on `Cellink`; the 2026-08-23 pull is a **fully-zero Algolia lane on all four vendor queries + both workflow queries**). A title/URL regex sweep across the 31 unique top+best items also turned up zero organoid / 3D-bioprinting / CelVivo / 3Brain / Cellink / ClinoStar / ClinoReactor / MEA / spheroid / organ-on-chip items. The "no material HN footprint for the 4 vendor lines" finding continues to hold — this is the **thirteenth pull since 2026-08-07 with an empty / noise-only Algolia lane on vendor queries** (after the 2026-08-08 03:15 + 2026-08-08 18:00 + 2026-08-13 + 2026-08-15 + 2026-08-16 + 2026-08-18 + 2026-08-19 + 2026-08-21 + 2026-08-23 fully-zero pulls + the 2026-08-10 + 2026-08-12 + 2026-08-17 + 2026-08-20 + 2026-08-22 noise-only pulls where vendor queries returned substring-fragment noise rather than fully zero; the 2026-08-23 pull returns to fully-zero across all four vendor queries + both workflow queries — **a stricter "all-zero Algolia lane" than the 2026-08-22 noise-only lane**, with no application-domain thematic hits to soften the absence; the 2026-08-21 + 2026-08-23 fully-zero pulls are bookends on the 2026-08-22 noise-only pull, confirming that the empty-pulls-on-vendor-queries cadence is normal under the 6-query LLM-agent topic set, not an outage, no config-side intervention needed). The Tavily (web-search) lane remains the productive lane for vendor news; the HN lane's stable role is now "high-engagement inference-lane / agent-stack adjacents (What Is a Harness? + GLM-5.3 cluster + local-LLM essay + Munder Difflin + New MCP Roadmap + OzBrain + DeepSeek V4 Flash vision + Proliferate + machine0 + OneCLI + OpenRouter-Stripe confirmation + Copilot Autofix + Unsloth Dynamic 3.0 GGUFs + AGENTS.md) + low-engagement application-domain watchlist items (organoid / neuroscience / Wired features) + low-engagement self-hosted-agentic-substrate cluster essays (DeepSeek Harness + machine0 + OneCLI + self-hosted factory + Munder Difflin + What Is a Harness?)" rather than vendor-direct signals. The three highest-engagement life-science-adjacent items on this pull all fall outside Robin's 4 application domains and stay off the `class-organoid-3d-bioprinting` watchlist per the established "domain-anchored only" scope rule: `49410149` "Vit D assoc w better cognition in those w sleep disturb./mild cognitive impair" (15 pts top, 5 cmts, sciencedirect.com — clinical nutrition paper on Vitamin D + cognition in sleep-disturbance / mild cognitive impairment; **adjacent-not-promoted** per scope because clinical nutrition is not one of Robin's 4 application domains — 3D cell culture, 3D bioprinting, neuroscience in vitro, organoid workflows — though the cognition + sleep-disturbance framing has a tangentially neuroscience-in-vitro adjacency); `49402232` "Why your local LLM feels dumber than it is" (450 pts top+best, 182 cmts — local-LLM optimization essay on Level1Techs forum; **adjacent-not-promoted** per scope but with a direct local-LLM-stack cross-walk to the `MiniMax-m3` row via `unsloth/MiniMax-M3-GGUF`, the strongest local-LLM-adjacent item of the pull); `49402189` "Scrap (2006)" (399 pts top+best, 216 cmts — Moxie Marlinspike (Signal founder) Twitter quote of a 2006 essay on trust + network architecture, off-scope per brief).

sources/index.md

@@ -4,7 +4,7 @@
 - [Agents 代理商档案索引 — 跨 agent CRM 同步](agents.md) - Cross-agent index of the Obsidian vault 02-Clients/Agents/* profiles (which agents Robin is actively working with, mapped by vendor product line). Base identity only — sensitive fields (phone / WeChat / email / end-customer list / opportunity value) live only in the private vault. Updated 2026-08-07 with _known_associations.md product-line → agent → end-customer mapping.
 - [全国经销商地图 (2026-08-07 时点)](distributors-china-2026.md) - Compact index of Robin's 2026-08-07 China distributor baseline (259 dealers across 7 regions, replacing 261 in the 2026-07-24 baseline) plus the new product-association index _known_associations.md. Surfaces CelVivo ClinoStar → 济南成全 + FemtoBiomed CellShot → 9X Bio → TriArm Therapeutics chain. Routes pricing-sensitive fields to the private Obsidian vault per /sources/agents.md privacy rule.
 - [飞书邮箱 (Robin customer mailbox)](feishu-mail.md) - Planned cross-agent mailbox sync target for Robin's Feishu (飞书) mailbox (imap.feishu.cn:993 / imap.larksuite.com:993) — would let MiniMax code / Hermes recall 'what did Robin last discuss with customer X' from email metadata + summaries. Not yet provisioned — IMAP credentials + update script + crontab still pending Robin setup; this page documents the planned configuration and the sync policy that will be applied once provisioned.
-- [Git repo — evidence index (Obsidian vault, Robin)](git-repo.md) - Compact manifest of Robin's Obsidian vault + the local OpenWiki wiki, ingested through the local git-repo connector. Two configured repos: obsidian-vault at /home/ldw/workspace (main branch, HEAD a4fa5a32, 2026-08-16, **0 new vault commits in the 2026-08-22T18:13Z → 2026-08-23T18:11Z 24-hour window — vault is silent for the 7th consecutive fetch after the 2026-08-16 Quintard + Guo cluster, 7 calendar days stale**) and openwiki-wiki at /home/ldw/.openwiki/wiki (main branch, HEAD a9568d8, 2026-08-23, **2 new automated wiki commits in the 2026-08-22T18:13Z → 2026-08-23T18:11Z window: `8f8f767` sources-sync 2026-08-23 + `a9568d8` daily update 2026-08-23 — the ninth consecutive sources-sync re-applying the 2026-08-15 count-data wipe pattern**). This page is a navigation index — content ingestion (paper deep reads, sales scripts, customer files) happens via the Hermes skill and RAGFlow, not here.
+- [Git repo — evidence index (Obsidian vault, Robin)](git-repo.md) - Compact manifest of Robin's Obsidian vault + the local OpenWiki wiki, ingested through the local git-repo connector. Two configured repos: obsidian-vault at /home/ldw/workspace (main branch, HEAD fb017e4f, 2026-08-23/24, **5 new vault commits in the 2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window — the largest single-day LDR burst since the 2026-07-29 Parate / Lee / Hodge cluster: `ad4abcde` Ajongbolo 2025 Cells brain organoid review + `5f02489a` Kim 2025 Nat Commun EGaIn 3D MEA + `40b357b8` Tian 2025 Nat Commun hiPSC epicardium mTOR + `f2118a62` Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid CFD + `fb017e4f` CelVivo App Note 2026 ClinoStar vs orbital shaker — 3 CelVivo-aligned papers (App Note + Aiello + Ajongbolo mentions ClinoStar) + 1 3Brain-adjacent 3D-MEA competitor paper (Kim EGaIn) + 1 cardiac epicardium paper (Tian)**) and openwiki-wiki at /home/ldw/.openwiki/wiki (main branch, HEAD 127c2dc, 2026-08-24, **2 new automated wiki commits in the 2026-08-23T18:11Z → 2026-08-24T18:05Z window: `9cc5e63` daily update 2026-08-24 + `127c2dc` sources-sync 2026-08-24 — the tenth consecutive sources-sync re-applying the 2026-08-15 count-data wipe pattern**). This page is a navigation index — content ingestion (paper deep reads, sales scripts, customer files) happens via the Hermes skill and RAGFlow, not here.
 - [Hacker News — evidence index](hackernews.md) - Compact evidence trail for the Hacker News connector (hackernews-1, Robin-curated life-sciences + AI workflow). Synthesis lives on /themes.md, /commitments.md, etc.; this page only records ingestion coverage and notable items.
 - [文献精读 Master Template (Robin 7-27 实战版, "真适合浏览" 1 文件)](ldr-report-template.md) - Master template for Robin's literature-deep-read (LDR) pipeline — consolidates 4 literature skills (`literature-deep-read` / `research` / `review` / `curation-for-sales`) into a single 8-rule + 3-trigger + 5-section "真适合浏览" report format. Synthesized from 4-27 SOP范本 + 7-27 实战 lessons (Tasevska / Raji / Bosnjakovic). Triggers "精读吧" / "发 wikijs" / "入库" / "A" / Id/DOI/PMID/PDF signals into 4-step精简 or 8-step完整 flows. PDF全文必传 + PyMuPDF xref去重 + 9项强制 + SeaFile hostname `fb.biokingdom.top` + NocoDB v1 JSON + 3张真图 + `journal-year-topic` 报告路径.
 - [Bosnjakovic 2025 PAH 3D 球体代谢组学: 跟 CelVivo ClinoStar dynamic culture 关系 (Bosnjakovic 2025 bioRxiv)](literature-cel-bosnjakovic-2025-pah-deepread.md) - Deep-read synthesis of Bosnjakovic A et al. 2025 bioRxiv preprint (DOI 10.1101/2025.07.10.663939v1, 2025-07-10) — 21-day mature HepG2 3D spheroids with graded non-cytotoxic BaP + BBF (PAH代表物) exposure for metabolomics biomarker discovery. Robin's connection: 3D spheroid protocol maps directly onto CelVivo ClinoStar dynamic culture; supports PAH exposure metabolomics positioning for hepatotoxicity / NAFLD modeling applications. 精读 2026-07-27.

sources/literature.md

@@ -9,10 +9,20 @@ tags: [literature, deep-read, flywheel, celvivo, 3brain, cellink, femtobiomed]
 
 > Source: Obsidian vault `04-Literature/Deep-Reads/<vendor>/<DOI>.md` (git-repo connector auto-sync) + flywheel-sync `~/.hermes/data_sources/literature_flywheel.jsonl` for pipeline status.
 > Purpose: allow MiniMax code / Hermes cross-agent retrieval of "what has Robin recently deep-read".
-> Update: OpenWiki cron runs at 02:00 daily; today's flywheel-sync (2026-08-08) has completed `flywheel_index` on 3 papers (Sharma 2026 Cell Stem Cell, Miao 2025 Cell v2, Liu 2026 Nat Biomed Eng) — see the new entries below.
+> Update: git-repo-1 2026-08-24 18:05 UTC — **5-paper root-level LDR burst** (CelVivo App Note + Aiello + Tian + Kim + Ajongbolo) — the largest single-day cluster since 2026-07-29; all 5 files landed at `04-Literature/Deep-Reads/` root (non-standard pattern vs the prior per-vendor subfolder discipline). Previous flywheel-sync (2026-08-08) completed `flywheel_index` on 3 papers (Sharma 2026 Cell Stem Cell, Miao 2025 Cell v2, Liu 2026 Nat Biomed Eng) — see entries below.
 
 ## Last 30 days of deep reads (mtime descending)
 
+### 2026-08-23 (5-paper single-shot LDR burst — vault root, non-standard pattern)
+
+| DOI / slug | Vendor | Application | Title (short) | Vault path |
+| --- | --- | --- | --- | --- |
+| `CelVivo_AppNote_2026` | **CelVivo (ClinoStar vs Orbital Shaker direct comparison)** | Low shear stress + cytoskeletal integrity for 3D constructs (HepG2/C3A 15d + embedded Caco-2 + brain organoid) | Stougaard Borgen Løschenkohl + Sedighi Frandsen, **CelVivo Application Note 2026** (sourced from RAGFlow CelVivo KB 1555e5b2, Robin 8-24 上传; Wiki.js 1747) — head-to-head **ClinoStar vs orbital shaker** comparison: ClinoStar preserves F-actin organization + cytoskeletal integrity + tight cellular cohesion vs orbital shaker's dispersed F-actin + loose morphology + reduced cohesion (phalloidin/DAPI dual-magnification 4x + 20x); 15-day standardized workflow with 3 bio-replicates; multi-model transferability across HepG2/C3A + embedded Caco-2 + brain organoid. **Canonical ClinoStar vs-orbital-shaker competitive-killer document**; Robin's first vendor App Note deep-read | `obsidian-vault/04-Literature/Deep-Reads/CelVivo_Shear_Stress_AppNote_2026.md` |
+| `biorxiv_Aiello_Bagni_2025_RC_CFD` | **CelVivo (ClinoStar-adjacent — RC = ClinoStar simplified geometry)** + 3Brain (cortical organoid e-phys, adjacent) | CFD-quantified low shear stress Rotating Chamber for brain organoid | Aiello F, Bagni C et al. (2025) **biorxiv preprint** (the EMBO Reports submission version `Aiello_Bagni_EMBO R_050325`, Robin 8-24 从 CelVivo KB 找到的 PDF; Wiki.js 1746) — **first CFD-quantified Rotating Chamber (RC) study**: cavity geometry Ø 37.7 mm × H 8.8 mm; **COMSOL Multiphysics 6.2 shows cavity shear stress < 20 mPa** (extremely low); multi-density 1010/1020/1030 vs medium 1000 confirms shear-stress uniformity; morphological CV < 15% vs static culture CV > 30% across batches + cell lines; cortical cell type recapitulation (SOX2/Nestin/PAX6 + DCX + TBR1/SATB2/MAP2 multi-marker IHC) + scRNA-seq OXPHOS metabolic profiling. **The < 20 mPa quantitative number is direct ammunition** for the existing ClinoStar vendor-positioning pitch | `obsidian-vault/04-Literature/Deep-Reads/Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid.md` |
+| `10.1038/s41467-025-60934-8` | (no vendor callout — cardiac application-domain) | hiPSC epicardium mTOR-driven maturation + paracrine cardiomyocyte cross-talk | Tian Y, Lucena-Cacace A, Tani K et al. (2025) **Generation of mature epicardium derived from human-induced pluripotent stem cells via inhibition of mechanistic target of rapamycin** (Nat Commun; Wiki.js 1745) — **Torin1 (dual mTORC1/2 inhibitor, 200 nM) drives hiPSC epicardium maturation**: mature markers (TJP1/WT1/TBX18/ALDH1A2/CDH18) up + EMT markers (SNAI1/SNAI2/CDH2/CDH1) down + proliferation markers (MKI67/PCNA/MCM2) down + fatty-acid metabolism maturation (PDK4/PPARGC1A/SIRT1/FASN/SREBF1); 379 DEGs; adult-like profile vs GSE84085; YBX3 + MAFF identified as Torin1-specific TFs; **paracrine TNNI3 upregulation in hiPSC ventricular cardiomyocyte EBs co-cultured for 10 days**. **First cardiac-epicardium paper in Robin's deep-read set** | `obsidian-vault/04-Literature/Deep-Reads/Tian_2025_NatCommun_Epicardium_mTOR.md` |
+| `10.1038/s41467-024-55752-3` | **3Brain (HD-MEA competitor / adjacent paper — 3rd next-gen HD-MEA approach)** | EGaIn liquid-metal 3D MEA + magnetically reshapable + cortical organoid electrophysiology | Kim E, Jeong E, Hong YM et al. (2025) **Magnetically reshapable 3D multi-electrode arrays of liquid metals for electrophysiological analysis** (Nat Commun; Wiki.js 1744) — microfluidic direct-ink-writing EGaIn 3D pillar MEA with **pillar height 25-310 μm (R² > 0.95, n=12)** + **pillar diameter 14 μm** (single-neuron scale) + parylene-C selective encapsulation; **Ni magnetic layer for magnetic reshape** → shape conforms to different organoid sizes; hPSC cortical organoid maturation 15/30/45/60/120 days → 6-marker co-expression (MAP2/GFAP/VGLUT1/SATB2/CTIP2) = cortical 6-layer-like structure; biocompatible 4-month organoid + 3D MEA co-culture 14d; 64-channel MEA single-unit spike + network synchronization. **3 distinct next-gen HD-MEA approaches now in Robin's deep-read set**: Liu 2026 Rogers Lab flexible-electronics framework + Kim 2025 EGaIn 3D pillar + 3Brain Accura-3D rigid CMOS | `obsidian-vault/04-Literature/Deep-Reads/Kim_2025_NatCommun_Liquid_Metal_3D_MEA.md` |
+| `10.3390/cells14110842` | **CelVivo (ClinoStar-adjacent) + 3Brain (HD-MEA-adjacent)** | Brain organoid + assembloid review (disease modeling + drug discovery) | Ajongbolo AO, Langhans SA (2025) **Brain Organoids and Assembloids—From Disease Modeling to Drug Discovery** (Cells; Wiki.js 1743) — **canonical brain organoid + assembloid review**: 3-tier taxonomy (whole-brain → region-specific → complex systems); 4-class characterization methodology (morphological + biochemical + functional + omics); disease-modeling boundaries (neurodevelopmental / neurodegenerative / brain-tumor); drug-discovery HTS pipeline (96-well + microfluidic + automated imaging + AI phenotype). **First comprehensive brain-organoid review** in Robin's deep-read set since the 2026-07-29 Organoids 2026 review — pairs with that review to anchor the brain-organoid review lane | `obsidian-vault/04-Literature/Deep-Reads/Ajongbolo_2025_Cells_Brain_Organoids_Review.md` |
+
 ### 2026-08-16 (Quintard 2024 + Guo 2026 — 2 papers on the same day)
 
 | DOI | Vendor | Application | Title (short) | Vault path |
@@ -232,3 +242,62 @@ The `Robin_Picked_Papers` table is **new as of today** — created per Robin's 2
 - **Business relevance**: 4 大产品线 (CelVivo ClinoStar / 3Brain MEA / Femtobiomed CellShot / Cellink BIO X) 全部可对接 AIVOs 平台 = 完整 湿+干 实验方案
 - **Tags**: 综述, 类器官, 人工智能, 虚拟器官, 数字孪生, 多组学, Bioactive-Materials-2026
 | 10.1038/s41598-026-42990-2 | Sci Rep 2026 | Liver | Liang 2026 小鼠肝类器官成熟+纤维化建模平台 | obsidian-vault/04-Literature/Deep-Reads/Liver/2026-08-12_Liang_2026_SciRep_liver_organoid_fibrosis_platform.md |
+
+## 2026-08-24 - Ajongbolo & Langhans 2025 Cells (PMID 40498018)
+- **DOI**: 10.3390/cells14110842 | **JIF**: 7.0 (Cells MDPI)
+- **Wiki.js**: https://wiki.biokingdom.top/literature/ajongbolo-2025-cells-brain-organoids-review (ID 1743)
+- **主题**: 脑类器官 + assembloid + 类器官芯片 + tumoroid 四大范式综述;神经发育/神经退行/脑肿瘤建模与高通量药物筛选
+- **关键发现**: 综述把脑类器官从分散成果整合成标准化决策树;神经发育建模最成熟(复现率 70%+),脑肿瘤个性化最高
+- **配图**: 2 张 (Fig 1 分类树 + Fig 2 表征方法分类) — Wiki.js /u 公网, ADOPT 2/2
+- **PDF**: NAS SMB fallback (8-18 坑 12 命中第 2 次, Cloudreve share 返 200 但公网 404)
+
+## 8-24 精读报告永久格式规则 (Robin 拍板)
+1. **不写本地副本路径** — 报告末尾 PDF 行后不再加 NAS SMB 路径
+2. **转发语加 "转发语:" 前缀** — 末尾 `> **转发语:**` 起头,不裸 `>`
+3. 这 2 条永久生效, 未来精读报告 + patch 必守
+
+## 2026-08-24 - Kim et al. 2025 Nat Commun (PMID 40016200)
+- **DOI**: 10.1038/s41467-024-55752-3 | **JIF**: 16.6 (Nat Commun)
+- **Wiki.js**: https://wiki.biokingdom.top/literature/kim-2025-natcommun-liquid-metal-3d-mea (ID 1744)
+- **主题**: 液态金属(EGaIn)3D pillar MEA + 磁可重塑 + 皮层类器官内部 SU 电生理记录 + 6 月成熟度量化
+- **关键发现**: 首次实现类器官**内部**单单元电生理记录 + 噪声 13 μV + 6 月成熟(spike rate↑, burst duration↑, p<0.05);磁场驱动 pillar 重塑 20 循环稳定
+- **配图**: 6 张 (Fig 1-6, ADOPT 6/6) — Wiki.js /u 公网
+- **PDF**: NAS SMB fallback (8-18 坑 12 第 3 次命中,Nature 系 frp/share-handler 系统性 bug)
+- **PDF 直链可用**: https://www.nature.com/articles/s41467-024-55752-3.pdf (Nature 子刊 PDF 公开访问)
+
+## 8-24 精读配图永久规则 (Robin 拍板)
+1. **多 panel 复合图必须整页渲染** — caption_y 裁剪到 caption 上方, 覆盖 panel 字母
+2. **嵌入大图 xref 仅用于单 panel 纯图像图** — 嵌入图本身不含文字标注
+3. **Springer Nature 系双栏全宽 Figure** — caption 在图下方, 整页渲染到 caption_y - 15
+4. **视觉审核 panel 数 ≥ 原文 caption 声明** — 是判 ADOPT 的硬阈值
+5. 这 4 条永久生效, 未来精读 + patch 必守
+
+## 8-24 v2 二次反馈: Springer Nature PDF 嵌入图只含部分 panel
+- 根因: PDF 嵌入图 xref 只含 raster 部分, 部分 panel 是矢量绘制 (PDF vector, 无 xref)
+- 修法: 每个 Figure 走"完整 page 渲染" — 从 page 顶部 (y=33 article header 后) 到 caption_y - 15
+- 实战: Kim 2025 Fig 5 嵌入图 xref 212 只含 f-j 网络图, 完整 Fig 5 还有 a-e SU trace + 柱状图 (5 个矢量绘制 panel)。Fig 5 v4: 228/50 ADOPT, 10/10 panels 全在
+- 这条永久生效, 未来精读 + patch 必守
+
+## 2026-08-24 - Tian et al. 2025 Nat Commun (PMID 40593704)
+- **DOI**: 10.1038/s41467-025-60934-8 | **JIF**: 16.6 (Nat Commun)
+- **Wiki.js**: https://wiki.biokingdom.top/literature/tian-2025-natcommun-epicardium-mtor (ID 1745)
+- **主题**: mTORC1/2 双抑制 (Torin1) 驱动 hiPSC 心外膜成熟, YBX3/MAFF TF 轴 + 3D 类器官 + 心肌旁分泌共培养
+- **关键发现**: 379 DEGs; 心外膜转录组从胎儿表型推到成体; mTORC2 是关键节点; 心外膜旁分泌 IGF2/FN1 推动心肌 TNNI3 成熟
+- **配图**: 7 张 (Fig 1-7, 4/7 ADOPT Fig 4-7, Fig 1-3 待限流补审) — v5 智能渲染 (含跨页拼接)
+- **PDF**: NAS SMB fallback (8-18 坑 12 第 4 次命中)
+
+## 2026-08-24 - Aiello & Bagni biorxiv preprint (CelVivo KB 来源)
+- **Title**: Design and validation of a low-shear Rotating Chamber system for brain organoid culture
+- **Source**: RAGFlow CelVivo KB (1555e5b2... 170 docs), Brain_Organoid_Fluid_biorxiv.pdf (14MB, 49 chunks)
+- **Wiki.js**: https://wiki.biokingdom.top/literature/aiello-bagni-2025-biorxiv-rotating-chamber-brain-organoid (ID 1746)
+- **主题**: CelVivo ClinoStar 旋转腔 COMSOL CFD 量化 (<20 mPa 剪切力) + 跨细胞系/批次形态变异系数 <15% + 完整皮层细胞类型与代谢成熟
+- **配图**: 5 张 (Fig 1-5, MiniMax 限流未审) — v3 拼接 (figure 在 page 40-44, caption 在 page 32-35, 跨页布局)
+- **PDF**: NAS SMB fallback
+
+## 2026-08-24 - CelVivo App Note shear stress (CelVivo KB 来源)
+- **Title**: Influence of shear stress on long-term 3D construct performance: Comparison of orbital shaker and ClinoStar system
+- **Source**: RAGFlow CelVivo KB (1555e5b2... 170 docs), Shear_Stress_OS_CelVivo_appnote.pdf (1.4MB, 11 chunks)
+- **Wiki.js**: https://wiki.biokingdom.top/literature/celvivo-appnote-shear-stress-2026 (ID 1747)
+- **主题**: CelVivo 官方 App Note — ClinoStar vs Orbital Shaker head-to-head 对比 HepG2/C3A + Caco-2 + 脑 organoid 三模型, 证明低剪切力优势跨细胞模型可复现
+- **配图**: 5 张 (Fig 1-5, MiniMax 限流未审) — v4 智能拼接 (xref bbox + caption 段)
+- **PDF**: NAS SMB fallback

sources/nocodb.md

@@ -1,3 +1,9 @@
+---
+type: "Reference"
+title: "NocoDB 文献库索引 — 跨 agent 数据层"
+openwiki_generated: true
+---
+
 # NocoDB 文献库索引 — 跨 agent 数据层
 
 > 数据源: NocoDB 13 个文献库 + 2 个公司库 (实时,每次 OpenWiki cron 跑时更新)

sources/ragflow.md

@@ -1,3 +1,9 @@
+---
+type: "Reference"
+title: "RAGFlow 全文检索索引 (Robin KB system)"
+openwiki_generated: true
+---
+
 # RAGFlow 全文检索索引 (Robin KB system)
 
 > 数据源: RAGFlow (http://10.10.10.254:9980/) 14 个 KB 全文检索结果

sources/seafile.md

@@ -1,3 +1,9 @@
+---
+type: "Reference"
+title: "SeaFile 大文件存储索引"
+openwiki_generated: true
+---
+
 # SeaFile 大文件存储索引
 
 > 数据源: SeaFile (http://10.10.10.254:9898/) 共 N 个资料库

sources/web-search.md

@@ -2,13 +2,21 @@
 type: "Reference"
 title: "Web Search Evidence"
 description: "Compact, source-backed evidence and ingestion coverage for the web-search-1 Tavily lane tracking CelVivo, 3Brain, Cellink, and related organoid news."
-tags: [source, web-search, tavily, celvivo, 3brain, cellink, organoids, 2026-08-12, 2026-08-15, 2026-08-16, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, 2026-08-23]
-timestamp: 2026-08-23T18:06:21Z
+tags: [source, web-search, tavily, celvivo, 3brain, cellink, organoids, 2026-08-12, 2026-08-15, 2026-08-16, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, 2026-08-23, 2026-08-24]
+timestamp: 2026-08-24T18:04:19Z
 ---
 
 # Web Search Evidence
 
-> Connector: `web-search-1` (Tavily). This page records compact, relevant evidence from Tavily pulls; search results are discovery leads, not vendor-confirmed announcements. Latest successful pull: 2026-08-03 18:08 UTC. The 2026-08-08, 2026-08-10, 2026-08-12, 2026-08-15, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, and 2026-08-23 update attempts were skipped because the connector is disabled and `TAVILY_API_KEY` is not present; no new 24-hour evidence was available on any of these eleven runs.
+> Connector: `web-search-1` (Tavily). This page records compact, relevant evidence from Tavily pulls; search results are discovery leads, not vendor-confirmed announcements. Latest successful pull: 2026-08-03 18:08 UTC. The 2026-08-08, 2026-08-10, 2026-08-12, 2026-08-15, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, 2026-08-23, and 2026-08-24 update attempts were skipped because the connector is disabled and `TAVILY_API_KEY` is not present; no new 24-hour evidence was available on any of these twelve runs.
+
+## 2026-08-24 attempted update — skipped
+
+- **Status:** skipped. `web-search-1` is not enabled; the connector reports that `enabled=true` must be set in `~/.openwiki/connectors/web-search/config.json`.
+- **Authentication:** `TAVILY_API_KEY` is not present, so no Tavily request was made.
+- **Coverage:** no raw data files were written for this attempted 24-hour window. The nightly searxng-fallback slot (`~/.openwiki/connectors/web-search/raw/2026-08-23T18-35-03-searxng-fallback/`) is also empty (confirmed empty on disk — no files in the directory) — no web-search evidence of any kind landed in the 24-hour window. No new CelVivo, 3Brain, Cellink, or organoid findings were available for synthesis; the latest successful pull remains 2026-08-03 18:08 UTC.
+- **Synthesis:** no changes were made to `/themes.md`, `/commitments.md`, `/open-questions.md`, or `/personal-logistics.md` from this source attempt.
+- **Operational note (cross-check):** the on-disk `~/.openwiki/connectors/web-search/config.json` does show `enabled: true` plus 6 Chinese-language queries (`CelVivo ClinoStar ClinoReactor 类器官培养新进展`, `3Brain HD-MEA 高密度微电极阵列 电生理`, `Cellink BIO X 3D生物打印 类器官`, `Femtobiomed CellShot 流式电转 精准医疗`, `类器官 vendor 2026年新发布`, `MiniMax M3 智能体 Hermes`). The agent runtime still reports the connector as disabled, so there is a discrepancy between disk config and runtime registry. This is an operational concern (connector enable / auth / re-registration), not a wiki-synthesis concern, and is recorded here for traceability rather than triggering wiki edits. The 2026-08-08, 2026-08-10, 2026-08-12, 2026-08-15, 2026-08-17, 2026-08-18, 2026-08-19, 2026-08-20, 2026-08-21, 2026-08-22, and 2026-08-23 runs hit the same blocker; this is the **twelfth consecutive skip on the same code path**. To resolve: set `TAVILY_API_KEY` in the OpenWiki runtime environment and ensure the runtime registry refreshes the connector's enabled state.
 
 ## 2026-08-23 attempted update — skipped
 

sources/wikijs.md

@@ -1,3 +1,9 @@
+---
+type: "Reference"
+title: "Wiki.js 索引 — 跨 agent 文档发布层"
+openwiki_generated: true
+---
+
 # Wiki.js 索引 — 跨 agent 文档发布层
 
 > 数据源: Wiki.js (http://10.10.10.254:3000/graphql) Biokingdom 实例

themes.md

@@ -13,7 +13,7 @@ Index kept narrow by design: only themes that map to Robin's actual work (life-s
 
 | Topic key | Theme / Signal | First seen | Last seen | Confidence | Sources | Evidence count | Status | Evidence |
 | --- | --- | --- | --- | --- | --- | --- | --- | --- |
-| `class-organoid-3d-bioprinting` | Recurring organoid, 3D bioprinting, and 3D-cell-culture automation coverage; this window adds a 2026-07-28 HN drug-discovery essay (owlposting "Why haven't organoids solved all of drug discovery?", 9 pts), a 2026-07-27 Oxford Academic review on 3D-bioprinted organoids, a 2026-07-22 Sage review on bioprinting-enabled organoids, a 2026-07-27 Pluristyx iPSC + aerospace clinical-grade hepatocyte manufacturing partnership, the first vendor-anchored CelVivo ClinoReactor co-marketing signal (TheWell Bioscience xeno-free workflow pages), the first vendor-anchored Cellink BIO X Gen 3 / BIO X6 signal (Nuclear Mechano-Oncology install + CELLINK LinkedIn U. Miami reference + third-party market report), and the third vendor-anchored Cellink signal — **CELLINK × Volumetric new Lumen X release** (3DPrint.com 2026-07-28) — plus GEN "Large Bioprinted Tissues Get a Precision Boost" (2026-07-29) and MDPI Cells "Rapid Volumetric Bioprinting Coupled with Dynamic Perfusion Enhances Human Hepatic Organoid Toxicity Testing" (2026-07-27) on top of the prior Nature / HN evidence; pull 7 (2026-07-30) adds FDA NAMs framework (2026-07-27, organ-on-chip included among New Approach Methodologies alongside June 2026 cell/gene-therapy draft guidance), Molecular Cancer patient-derived 3D-bioprinted primary liver cancer (Peking Union Medical College Hospital, 2026-07-28), and npj Biomedical Innovations microvasculature bioprinting review (2026-07-29); pull 11 (2026-08-03) re-anchors the HepatoBiliary Surgery and Nutrition "Spatial architecture and dynamic surveillance: 3D bioprinting and microfluidics converge" review (2026-08-01) as the strongest same-week cross-anchor between organ-on-chip and 3D-bioprinting; **flywheel-sync 2026-08-08** adds 3 deep-read papers: Liu 2026 Nat Biomed Eng (shape-conformal porous framework for full-coverage neural organoid e-phys, Rogers Lab — microlattice fabrication rate 5 for Cellink, HD-MEA rate 5 for 3Brain), Miao 2025 Cell v2 (co-differentiation mesoderm+endoderm for vascularized lung/gut organoids — ClinoStar rate 5, organotypic vascularization), Sharma 2026 Cell Stem Cell (vascularized retinal organoid RGC survival — HD-MEA rate 4, CelVivo ClinoStar rate 3 for extending vascular network stability); still mostly application-domain plus three vendor-channel signals (CelVivo+TheWell; Cellink BIO X Gen 3 / BIO X6; Cellink Lumen X) + three literature flywheel-sync application-domain signals (Liu/Miao/Sharma); **2026-08-12 HN pull** adds item `49266068` "AI Is Dead. Organoids Are Alive" (5 pts, Wired, by `Logans_Run`) on lab-grown brain organoids vs silicon AI — application-domain reinforcement of the organoid + neuroscience-in-vitro intersection that the 2026-07-27 Nature biocomputing article (`49071596`) and the 2026-07-28 owlposting drug-discovery essay (`49083799`) already speak to; **2026-08-20 HN pull** adds item `49366519` "Human brain organoids record the passage of time over multiple years" (18 pts top, by `sbulaev`, https://www.nature.com/articles/s41586-026-10877-x) — first new organoid + neuroscience-in-vitro thematic signal since the 2026-08-12 `49266068` Wired `AI Is Dead. Organoids Are Alive` entry, recording that human brain organoids maintained in long-term culture develop signatures of multi-year temporal processing — directly relevant to CelVivo ClinoReactor's long-term stable organoid culture value proposition; cross-walked under `49373812` "Scientists Have Built Miniature Brains That Experience the Passage of Time" (2 pts top, by `geox`, https://www.sciencealert.com/unprecedented-scientists-have-built-miniature-brains-that-experience-the-passage-of-time — Science Alert user-press write-up of the same Nature s41586-026-10877-x paper); **second neural-organoid HN signal in 9 pulls** (after the 2026-07-27 Nature biocomputing article `49071596` and the 2026-08-12 Wired `49266068`), qualifying for Last-seen refresh by the recurrence criterion alone | 2026-07-16 | 2026-08-20 | source-backed | hackernews, web-search, literature-flywheel | 28+ | active | [HN: 49083799 drug-discovery essay 2026-07-28](/sources/hackernews.md); [HN: 49266068 Wired "AI Is Dead. Organoids Are Alive" 2026-08-12](/sources/hackernews.md#class-organoid-3d-bioprinting-refreshed-this-run); [HN: 49366519 Nature brain-organoid time-passage paper 2026-08-20](/sources/hackernews.md#flag-for-traceability-nature-brain-organoid-time-passage-paper-in-scope-organoid-theme-refresh-anthropic-claude-code-agentsmd-feature-request-openwik-agentsmd-standard-cross-walk-unsloth-dynamic-30-ggufs-minimax-m3-gguf-upstream-science-alert-miniature-brains-organoid-paper-cross-walked-under-49366519--adjacent--1-promoted-to-class-organoid-3d-bioprinting-last-seen); [Tavily: 3D bioprinted organoid reviews + Pluristyx + CelVivo+TheWell 2026-07-27](/sources/web-search.md#2026-07-27-1804-pull); [Tavily: Cellink BIO X Gen 3 / BIO X6 + CN Bio / NASA AVATAR OOC + GEN bioprinting 2026-07-29](/sourcesLine truncated
+| `class-organoid-3d-bioprinting` | Recurring organoid, 3D bioprinting, and 3D-cell-culture automation coverage; this window adds a 2026-07-28 HN drug-discovery essay (owlposting "Why haven't organoids solved all of drug discovery?", 9 pts), a 2026-07-27 Oxford Academic review on 3D-bioprinted organoids, a 2026-07-22 Sage review on bioprinting-enabled organoids, a 2026-07-27 Pluristyx iPSC + aerospace clinical-grade hepatocyte manufacturing partnership, the first vendor-anchored CelVivo ClinoReactor co-marketing signal (TheWell Bioscience xeno-free workflow pages), the first vendor-anchored Cellink BIO X Gen 3 / BIO X6 signal (Nuclear Mechano-Oncology install + CELLINK LinkedIn U. Miami reference + third-party market report), and the third vendor-anchored Cellink signal — **CELLINK × Volumetric new Lumen X release** (3DPrint.com 2026-07-28) — plus GEN "Large Bioprinted Tissues Get a Precision Boost" (2026-07-29) and MDPI Cells "Rapid Volumetric Bioprinting Coupled with Dynamic Perfusion Enhances Human Hepatic Organoid Toxicity Testing" (2026-07-27) on top of the prior Nature / HN evidence; pull 7 (2026-07-30) adds FDA NAMs framework (2026-07-27, organ-on-chip included among New Approach Methodologies alongside June 2026 cell/gene-therapy draft guidance), Molecular Cancer patient-derived 3D-bioprinted primary liver cancer (Peking Union Medical College Hospital, 2026-07-28), and npj Biomedical Innovations microvasculature bioprinting review (2026-07-29); pull 11 (2026-08-03) re-anchors the HepatoBiliary Surgery and Nutrition "Spatial architecture and dynamic surveillance: 3D bioprinting and microfluidics converge" review (2026-08-01) as the strongest same-week cross-anchor between organ-on-chip and 3D-bioprinting; **flywheel-sync 2026-08-08** adds 3 deep-read papers: Liu 2026 Nat Biomed Eng (shape-conformal porous framework for full-coverage neural organoid e-phys, Rogers Lab — microlattice fabrication rate 5 for Cellink, HD-MEA rate 5 for 3Brain), Miao 2025 Cell v2 (co-differentiation mesoderm+endoderm for vascularized lung/gut organoids — ClinoStar rate 5, organotypic vascularization), Sharma 2026 Cell Stem Cell (vascularized retinal organoid RGC survival — HD-MEA rate 4, CelVivo ClinoStar rate 3 for extending vascular network stability); still mostly application-domain plus three vendor-channel signals (CelVivo+TheWell; Cellink BIO X Gen 3 / BIO X6; Cellink Lumen X) + three literature flywheel-sync application-domain signals (Liu/Miao/Sharma); **2026-08-12 HN pull** adds item `49266068` "AI Is Dead. Organoids Are Alive" (5 pts, Wired, by `Logans_Run`) on lab-grown brain organoids vs silicon AI — application-domain reinforcement of the organoid + neuroscience-in-vitro intersection that the 2026-07-27 Nature biocomputing article (`49071596`) and the 2026-07-28 owlposting drug-discovery essay (`49083799`) already speak to; **2026-08-20 HN pull** adds item `49366519` "Human brain organoids record the passage of time over multiple years" (18 pts top, by `sbulaev`, https://www.nature.com/articles/s41586-026-10877-x) — first new organoid + neuroscience-in-vitro thematic signal since the 2026-08-12 `49266068` Wired `AI Is Dead. Organoids Are Alive` entry, recording that human brain organoids maintained in long-term culture develop signatures of multi-year temporal processing — directly relevant to CelVivo ClinoReactor's long-term stable organoid culture value proposition; cross-walked under `49373812` "Scientists Have Built Miniature Brains That Experience the Passage of Time" (2 pts top, by `geox`, https://www.sciencealert.com/unprecedented-scientists-have-built-miniature-brains-that-experience-the-passage-of-time — Science Alert user-press write-up of the same Nature s41586-026-10877-x paper); **second neural-organoid HN signal in 9 pulls** (after the 2026-07-27 Nature biocomputing article `49071596` and the 2026-08-12 Wired `49266068`), qualifying for Last-seen refresh by the recurrence criterion alone; **2026-08-24 git-repo-1 LDR burst** adds 5 root-level single-shot entries that **strengthen the class-organoid-3d-bioprinting evidence footprint**: (a) **CelVivo App Note 2026 shear stress ClinoStar vs orbital shaker** — CelVivo-direct competitive-killer document (HepG2/C3A 15d + embedded Caco-2 + brain organoid); (b) **Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid CFD** — first CFD-quantified RC shear-stress study (< 20 mPa, CelVivo-adjacent); (c) **Ajongbolo 2025 Cells brain organoid + assembloid review** — canonical brain-organoid review (CelVivo ClinoStar-adjacent + 3Brain HD-MEA-adjacent), first comprehensive brain-organoid review since the 2026-07-29 Organoids 2026 review; (d) **Kim 2025 Nat Commun EGaIn liquid-metal 3D MEA** — 3rd next-gen HD-MEA approach alongside Liu 2026 + 3Brain Accura-3D (3Brain-adjacent competitor); (e) **Tian 2025 Nat Commun hiPSC epicardium mTOR maturation** — cardiac-epicardium application-domain expansion (no vendor callouts). **3 of 5Line truncated
 | `china-cde-cell-gene-therapy-rules-2026` | China CDE (under NMPA) published technical guidelines defining scope & classification of cell and gene therapy drugs; standardizes CMC planning for Chinese pharma/CDMO/biotech customers | 2026-07-24 | 2026-07-24 | source-backed | web-search | 1 | watchlist | [Tavily: cisema.com 2026-07-24](/sources/web-search.md) |
 | `china-order-no-818-clinical-translation` | China's Order No. 818 (effective 2026-05-01) creates an accelerated pathway for advanced biomedical technologies — including cell therapy and 3D bioprinting — towards regulated clinical translation at qualified hospitals after demonstrating safety and preliminary efficacy; the clinical-translation counterpart to the CDE classification item and directly relevant to Robin's Chinese pharma R&D / CDMO / biotech customer base | 2026-07-28 | 2026-07-28 | source-backed | web-search | 1 (+ Tavily synthesis) | watchlist | [Tavily: BetaLife LinkedIn 2026-07 + Roots Analysis re-mention 2026-07-28](/sources/web-search.md) |
 | `MiniMax-m3` | MiniMax-M3 (1M context multimodal) HuggingFace model card + Twitter launch; the daily-driver inference provider behind every OpenWiki run and most of Robin's agent work; haimaker.ai July 2026 third-party eval ranks models for Hermes Agent usage; MyClaw.ai 2026-07-29 confirms M3 + M2.7 are the operational models for both OpenClaw and Hermes, with M3 supporting multimodal (image, video, music, speech, web search); pull 11 (2026-08-03) adds **unsloth/MiniMax-M3-GGUF** (HuggingFace 2026-08-01) confirming quantized local-LLM run path + **benchlm.ai "DeepSeek V4 Flash vs MiniMax M3: Benchmarks & Cost"** (2026-08-01) reframing M3 as "1M context window at $0.30…"; this 2026-08-03 HN pull surfaces the **MiniMax-H3 launch cluster** (item `49155629` 167pts top-feed ComfyUI Day-0 support with open weights + native audio + 2K video; item `49150583` 8pts "MiniMax-H3 weights are up" on HuggingFace model card; item `49150861` 2pts "Run MiniMax-H3 Locally with SGLang Diffusion on 2× RTX 5090s or 1× RTX Pro 6000" via LMSYS Twitter) — three distinct primary sources on the same day confirming H3 as the next frontier model release after M3 | 2026-07-16 | 2026-08-03 | source-backed | hackernews, web-search | 8+ | active | [HN: M3 model cluster + H3 launch cluster 2026-08-03](/sources/hackernews.md); [Tavily: Hermes Agent best-models eval](/sources/web-search.md); [Tavily: MyClaw OpenClaw vs MiniMax Agent 2026-07-29](/sources/web-search.md#2026-07-29-1803-pull); [Tavily: unsloth/MiniMax-M3-GGUF + BenchLM M3 vs DeepSeek V4 Flash 2026-08-03](/sources/web-search.md#2026-08-03-1808-pull) |

themes/05-robin-research.md

@@ -1,9 +1,9 @@
 ---
 type: "Reference"
 title: "Theme 5 · Robin's personal research (vendor deep reads + sales ammunition + CRM)"
-description: "Synthesis page for Robin's personal research portfolio — Obsidian vault + literature deep-read pipeline + sales ammunition + China distributor map + active CRM profiles + work-rhythm snapshots. Output routes into vendor themes 01 (organoid-equipment) and 02 (bioprinting)."
-tags: [theme, research, vault, deep-read, sales, crm, life-sciences, 2026-08-23, 2026-08-22, 2026-08-21, 2026-08-20, 2026-08-19, 2026-08-18, 2026-08-17, 2026-08-15, 2026-08-13]
-timestamp: 2026-08-23T18:11:00Z
+description: "Synthesis page for Robin's personal research portfolio — Obsidian vault + literature deep-read pipeline + sales ammunition + China distributor map + active CRM profiles + work-rhythm snapshots. Output routes into vendor themes 01 (organoid-equipment) and 02 (bioprinting). The 2026-08-23/24 fetch surfaced the largest single-day LDR burst since 2026-07-29 — 5 papers committed to main + new `deepread_40240593/` pipeline workspace directory."
+tags: [theme, research, vault, deep-read, sales, crm, life-sciences, 2026-08-24, 2026-08-23, 2026-08-22, 2026-08-21, 2026-08-20, 2026-08-19, 2026-08-18, 2026-08-17, 2026-08-15, 2026-08-13, celvivo-app-note-2026, aiello-bagni-rc-cfd, tian-epicardium-mtor, kim-egain-3d-mea, ajongbolo-brain-organoid-review]
+timestamp: 2026-08-24T18:05:00Z
 ---
 
 # Theme 5 · Robin's personal research (vendor deep reads + sales ammunition + CRM)
@@ -51,19 +51,19 @@ The diagram shows Robin's research substrate: a single Obsidian vault with 8 buc
 
 ## Knowledge substrate: Obsidian vault
 
-Snapshot from [`/sources/git-repo.md`](/sources/git-repo.md) (last fetch 2026-08-23T18:11:00Z):
+Snapshot from [`/sources/git-repo.md`](/sources/git-repo.md) (last fetch 2026-08-24T18:05:00Z):
 
 | Field | Value |
 | --- | --- |
 | Repo path | `/home/ldw/workspace` |
 | Branch | `main` |
-| HEAD | `a4fa5a323d6dcabeb9eccad49e636ee6ba5f87a6` (**unchanged** at the 2026-08-23T18:11Z fetch — **0 new vault commits** in the 2026-08-22T18:13Z → 2026-08-23T18:11Z 24-hour window; vault is silent for the **7th consecutive fetch** after the 2026-08-17T18:09Z + 2026-08-18T18:10Z + 2026-08-19T18:08Z + 2026-08-20T18:09Z + 2026-08-21T18:09Z + 2026-08-22T18:13Z silent windows; **7 calendar days stale** since the last vault HEAD bump on 2026-08-16; the 2026-08-16 Quintard 2024 Nat Commun 4-commit LDR cycle + Guo 2026 Front Cell Dev Biol 3-commit LDR cycle remain the most recent vault activity) |
-| Ahead of origin/main | 20 commits new since 2026-08-03 fetch (unchanged from the 2026-08-17 + 2026-08-18 + 2026-08-19 + 2026-08-20 + 2026-08-21 + 2026-08-22 + 2026-08-23 fetches) |
+| HEAD | `fb017e4fb40f874e54fb2a92eff235abe1c487cb` (**bumped** at the 2026-08-24T18:05Z fetch — **5 new vault commits** in the 2026-08-23T18:11Z → 2026-08-24T18:05Z 24-hour window; **breaks the 7-fetch vault-silence streak** that started after the 2026-08-16 Quintard + Guo cluster; **largest single-day LDR burst since the 2026-07-29 Parate / Lee / Hodge cluster**) |
+| Ahead of origin/main | 25 commits new since 2026-08-03 fetch (+5 from 2026-08-24) |
 | Auth | local path only — no remote, no credentials |
-| Working-tree state | dirty (23 untracked files + 2 new untracked directories — `celvivo_commercial/1331651_20260630_131201.pdf` + `templates/类器官IRB申请清单.md` — both untracked; the `Review/zhou2023_computer_vision_microfluidics.md` first-entry-in-Review/-subfolder is still-uncommitted, carrying forward from the 2026-08-13 fetch, **12-day carry-forward** at the 2026-08-23T18:11Z fetch — **10 fetches silent** on the file) |
-| Raw manifest | `~/.openwiki/connectors/git-repo/raw/2026-08-23T18-11-34-883Z/manifest.json` |
-| **Latest cluster (2026-08-16, replacing the 2026-08-12 Liang 2026 cluster)** | **Quintard 2024 Nat Commun + Guo 2026 Front Cell Dev Biol 7-commit LDR cluster** (`0fc8db24` Quintard adds file 189 lines → `290e9635` -v2 → `24c713de` -v3 → `4073abf4` -v4 → `2e6f5989` Guo adds file 170 lines → `00f824f2` -v2 → `a4fa5a32` -v3) — **net-new external evidence** (DOI 10.1038/s41598-026-42990-2, PMID 41857098, mouse liver 3-轨道 Hep-Orgs + Cho-Orgs + HSC + DAPT+Dex P1-P10 长程成熟 + TGFβ 激活体外纤维化模型) |
-| **Latest uncommitted artifact (2026-08-13 → 2026-08-23, multi-file, unchanged at the 2026-08-23T18:11Z fetch)** | `04-Literature/Deep-Reads/Review/zhou2023_computer_vision_microfluidics.md` (carry-forward untracked from 2026-08-13, now at **12-day carry-forward** at the 2026-08-23 fetch — file mtime `2026-08-11 16:31:54 +0800` so mtime-based carry-forward is 12 days; first-observation-date-based carry-forward is 10 days; **structural signal continuing to weaken** per `/sources/git-repo.md` Notable-entity entry — 10 consecutive fetches silent on the file; subfolder is more likely a one-off than a recurring pattern) + **2 new untracked directories added at the 2026-08-17 fetch**, still-uncommitted at the 2026-08-23 fetch (**7-day carry-forward** each — was 6-day at the 2026-08-22 fetch, 5-day at the 2026-08-21 fetch): `celvivo_commercial/1331651_20260630_131201.pdf` (6-30 quote PDF migrated into new folder) + `templates/类器官IRB申请清单.md` (180-line IRB application checklist derived from Guo 2026; Robin's first customer-facing template produced from a single deep-read — see the new Templates subtree below) |
+| Working-tree state | dirty (23 untracked files + 3 untracked directories — `celvivo_commercial/1331651_20260630_131201.pdf` + `templates/类器官IRB申请清单.md` + `deepread_40240593/` (NEW this fetch, 7 subdirs); the `Review/zhou2023_computer_vision_microfluidics.md` first-entry-in-Review/-subfolder is still-uncommitted, carrying forward from the 2026-08-13 fetch, **13-day carry-forward** at the 2026-08-24T18:05Z fetch — **11 fetches silent** on the file) |
+| Raw manifest | `~/.openwiki/connectors/git-repo/raw/2026-08-24T18-05-31-919Z/manifest.json` |
+| **Latest cluster (2026-08-23/24, replacing the 2026-08-16 Quintard + Guo cluster)** | **5-paper root-level single-shot LDR burst** (`ad4abcde` Ajongbolo 2025 Cells brain organoid review → `5f02489a` Kim 2025 Nat Commun EGaIn 3D MEA → `40b357b8` Tian 2025 Nat Commun hiPSC epicardium mTOR → `f2118a62` Aiello & Bagni 2025 biorxiv Rotating Chamber brain organoid CFD → `fb017e4f` CelVivo App Note 2026 shear stress ClinoStar vs orbital shaker) — all 5 files committed to `obsidian-vault/04-Literature/Deep-Reads/` root (not in per-vendor subfolder, **non-standard pattern** vs the prior per-vendor subfolder discipline); all 5 single-shot entries (no v2/v3 SOP rewrite cycles this cluster, unlike the 2026-08-16 Quintard 4-commit + Guo 3-commit pattern). Vendor mapping: 3 CelVivo-aligned (App Note + Aiello + Ajongbolo review) + 1 3Brain-adjacent (Kim EGaIn 3D MEA) + 1 cardiac epicardium (Tian mTOR, no vendor callouts) |
+| **Latest uncommitted artifact (2026-08-13 → 2026-08-24, multi-file, unchanged at the 2026-08-24T18:05Z fetch except for new `deepread_40240593/`)** | `04-Literature/Deep-Reads/Review/zhou2023_computer_vision_microfluidics.md` (carry-forward untracked from 2026-08-13, now at **13-day carry-forward** at the 2026-08-24 fetch — file mtime `2026-08-11 16:31:54 +0800` so mtime-based carry-forward is 13 days; first-observation-date-based carry-forward is 11 days; **structural signal continuing to weaken** per `/sources/git-repo.md` Notable-entity entry — 11 consecutive fetches silent on the file; subfolder is more likely a one-off than a recurring pattern) + **2 prior untracked directories added at the 2026-08-17 fetch**, still-uncommitted at the 2026-08-24 fetch (**8-day carry-forward** each — was 7-day at the 2026-08-23 fetch, 6-day at the 2026-08-22 fetch, 5-day at the 2026-08-21 fetch): `celvivo_commercial/1331651_20260630_131201.pdf` (6-30 quote PDF migrated into new folder) + `templates/类器官IRB申请清单.md` (180-line IRB application checklist derived from Guo 2026; Robin's first customer-facing template produced from a single deep-read — see the new Templates subtree below) + **1 new untracked directory added at the 2026-08-24 fetch** — `deepread_40240593/` (7 subdirs: 5 PMID-named `deepread_<PMID>/` + 2 slug-named `deepread_brain_organoid_fluid/` + `deepread_shear_stress_cs_os/` that exactly map to the Aiello + CelVivo App Note papers; the new directory is Robin's LDR pipeline intermediate-artifact workspace, see `/sources/git-repo.md` Notable-entity § "deepread_40240593/ pipeline workspace") |
 
 ### Vault navigation index (top-level folders)
 
@@ -98,6 +98,7 @@ Snapshot from [`/sources/git-repo.md`](/sources/git-repo.md) (last fetch 2026-08
 | `Cellink/` | 129 | Cellink vendor + Cellink-adjacent papers |
 | `Organoid-List/` | 51 | curated organoid reading lists |
 | `Product-Reports/` | 38 | vendor product reports |
+| `04-Literature/Deep-Reads/` root | +5 from 2026-08-23/24 single-shot LDR burst (CelVivo App Note + Aiello + Tian + Kim + Ajongbolo) — **non-standard pattern** vs prior per-vendor subfolder discipline |
 | `Brain/` | 11 | brain organoid focus |
 | `Brain-Tumor/` | 9 | brain tumor organoid |
 | `Cancer/` | 6 | cancer organoid |

themes/index.md

@@ -4,4 +4,4 @@
 - [Theme 2 · 3D bioprinting (Cellink / BIONOVA)](02-bioprinting.md) - Synthesis page for Robin's 3D bioprinting theme — Cellink BIO X / BIONOVA X / Lumen X extrusion and DLP printing hardware for tissue engineering, drug tablets, and scaffold fabrication.
 - [Theme 3 · AI Agent tools (Hermes / Codex / MiniMax code)](03-ai-agents.md) - Synthesis page for Robin's daily-driver AI agent stack — Hermes Agent on WSL, MiniMax code on the desktop, Codex CLI fallback, OpenWiki as cross-agent memory substrate, and supporting toolchain (RustDesk, Gitea, Wiki.js, RAGFlow).
 - [Theme 4 · Local LLM deployment (Ollama / bge-reranker / RAGFlow)](04-local-llm.md) - Synthesis page for Robin's local-LLM fleet — Ollama serve + qwen2.5vl:7b vision model + BAAI/bge-m3 embeddings (Ollama + LM Studio) + bge-reranker rerank + RAGFlow KB retrieval + adjacent NAS subsystems (NocoDB / SeaFile / Wiki.js / Feishu mail).
-- [Theme 5 · Robin's personal research (vendor deep reads + sales ammunition + CRM)](05-robin-research.md) - Synthesis page for Robin's personal research portfolio — Obsidian vault + literature deep-read pipeline + sales ammunition + China distributor map + active CRM profiles + work-rhythm snapshots. Output routes into vendor themes 01 (organoid-equipment) and 02 (bioprinting).
+- [Theme 5 · Robin's personal research (vendor deep reads + sales ammunition + CRM)](05-robin-research.md) - Synthesis page for Robin's personal research portfolio — Obsidian vault + literature deep-read pipeline + sales ammunition + China distributor map + active CRM profiles + work-rhythm snapshots. Output routes into vendor themes 01 (organoid-equipment) and 02 (bioprinting). The 2026-08-23/24 fetch surfaced the largest single-day LDR burst since 2026-07-29 — 5 papers committed to main + new `deepread_40240593/` pipeline workspace directory.