chore: daily update 2026-08-25

commitments.md

@@ -123,6 +123,41 @@ Concrete work tasks, follow-ups, approvals, and scheduled items extracted from G
 - Status: open
 - Notes: **First customer-facing template produced from a single deep-read** in Robin's vault — the IRB application checklist is the canonical "Robin 培训客户 IRB 申请" core material for class A tertiary hospitals running organoid IRB submissions. The 7 mandatory points + 5 customer Q&A + China vs International flow table + 10 attachments + 7 citations map directly to the CelVivo ClinoStar clinical-translation sales narrative (Guo 2026 lists CelVivo ClinoStar ⭐⭐⭐⭐ for IRB compliance ammunition). **Three follow-ups needed**: (a) **commit** `templates/类器官IRB申请清单.md` to `main` (currently untracked); (b) add a wiki-side deep-read synthesis page at `/sources/literature-guo-2026-front-cell-dev-biol-organoid-ethics-deepread.md` mirroring the pattern at `/sources/literature-liu-2026-nbme-shape-conformal-organoid-deepread.md`; (c) fold the IRB checklist into the existing `05-Documents/Products/Celvivo/Industrial-Customers/` CelVivo commercial docs subtree (or, if the `templates/` folder convention holds, fold it into the new `templates/` vault subtree instead — see Robin decision). Companion commitment to fold into Chinese customer IRB conversations: the checklist covers China《人类遗传资源管理条例》+ 《涉及人的生命科学和医学研究伦理审查办法》2023 — directly applicable to the next Class A tertiary hospital Robin visits for CelVivo or 3Brain training.
 
+### celvivo-app-note-2026-clinostar-vs-orbital-shaker-positioning: fold the head-to-head shear-stress comparison into ClinoStar sales narrative + CelVivo赋能培训
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `fb017e4f` (CelVivo Application Note 2026 shear stress ClinoStar vs orbital shaker, Wiki.js 1747, sourced from RAGFlow CelVivo KB 1555e5b2..., Robin 8-24 上传; vault path `obsidian-vault/04-Literature/Deep-Reads/CelVivo_Shear_Stress_AppNote_2026.md`, 10,145 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **Canonical ClinoStar vs-orbital-shaker competitive-killer document** — the App Note establishes head-to-head comparison of ClinoStar® vs orbital shaker for 3D 类器官/球体培养 (HepG2/C3A 15-day + embedded Caco-2 肠 + brain organoid) with 3 bio-replicates per condition. Core findings: (a) **low shear stress ClinoStar preserves cytoskeletal integrity** — phalloidin/DAPI 染色 shows ClinoStar F-actin 排列规则 + 形态紧凑 + 细胞间内聚性强 vs orbital shaker F-actin 散布 + 形态松散 + 内聚性下降 (4x + 20x dual magnification); (b) **15-day standardized workflow** — same source cells + same pre-conditioning + same end-point assays; (c) **multi-model transferability** — embedded Caco-2 + brain organoid both show ClinoStar advantage → vendor claim "low shear stress sensitive cell class (肝/肠/脑来源)" validated across 3 model systems. **Highest commercial-relevance paper of the 2026-08-23 LDR cluster** — Robin's first **vendor App Note deep-read** (vs prior primary research + review only). Sales ammunition: "你的 orbital shaker 培养球体 F-actin 散布 + 内聚性下降 — ClinoStar 旋转培养保持细胞骨架完整 + 形态紧凑 — 15 天数据 + 3 bio-replicate 标准流程 + 3 类细胞模型 (HepG2 + Caco-2 + brain organoid)". Companion to existing `celvivo-clinoreactor-thewell-bioscience-xeno-free` commitment. Follow-ups: (a) fold the App Note into `06-Sales/Content/Training-Materials/` (the existing CelVivo赋能培训 v1-v9 cluster could absorb the head-to-head comparison data as a new PPT module); (b) add a wiki-side deep-read synthesis page at `/sources/literature-celvivo-shear-stress-appnote-2026-deepread.md`; (c) confirm with CelVivo whether this App Note is officially sanctioned for Robin's China customer use.
+
+### aiello-2025-rc-cfd-clinostar-positioning: fold the < 20 mPa quantitative CFD number into CelVivo ClinoStar sales narrative
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `f2118a62` (Aiello & Bagni 2025 biorxiv preprint Rotating Chamber brain organoid CFD, Wiki.js 1746, the EMBO Reports submission version `Aiello_Bagni_EMBO R_050325`, Robin 8-24 从 CelVivo KB 找到的 PDF; vault path `obsidian-vault/04-Literature/Deep-Reads/Aiello_Bagni_2025_biorxiv_Brain_Organoid_Fluid.md`, 11,731 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **First CFD-quantified Rotating Chamber (RC) low-shear-stress study** in Robin's deep-read set — the paper quantifies what prior ClinoStar marketing material only described qualitatively. Core finding: **COMSOL Multiphysics 6.2 CFD simulation shows cavity shear stress < 20 mPa** (extremely low vs. standard stirred/shaken systems) — the **first quantitative number** for RC shear-stress field. Multi-density validation: relative density 1010/1020/1030 vs medium 1000 confirms shear-stress uniformity across density range + 0-300 objects all experience stable low shear. Morphological CV < 15% across batches + cell lines vs static culture CV > 30%. Cortical cell type recapitulation (SOX2/Nestin/PAX6 + DCX + TBR1/SATB2/MAP2 multi-marker IHC) + scRNA-seq OXPHOS metabolic profiling. **Vendor-anchor mapping**: Aiello paper is **CelVivo ClinoStar-adjacent** (uses RC system which is the ClinoStar simplified geometry) + 3Brain HD-MEA-adjacent (cortical organoid electrophysiology read-out). Sales ammunition: "你的类器官培养系统剪切力是多少? 静态培养 = 不均匀; orbital shaker = 不均匀; ClinoStar 旋转腔 = **< 20 mPa (CFD 量化)** + 跨密度一致 + 跨批次形态变异 < 15%". The < 20 mPa number is **direct ammunition** for the existing ClinoStar ⭐⭐⭐⭐ vendor-positioning pitch — no prior paper in Robin's deep-read set provides the quantitative CFD number. Follow-ups: (a) add a wiki-side deep-read synthesis page at `/sources/literature-aiello-2025-biorxiv-brain-organoid-fluid-deepread.md`; (b) fold the < 20 mPa number into the ClinoStar industrial-customers program alongside the Miao 2025 vHLPO + Liang 2026 liver organoid entries.
+
+### kim-2025-egain-3d-mea-vs-3brain-positioning: track the next-gen HD-MEA field consolidation (Liu 2026 Rogers Lab + Kim 2025 EGaIn + 3Brain Accura-3D)
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `5f02489a` (Kim E, Jeong E, Hong YM et al. 2025 Nat Commun liquid-metal 3D MEA, DOI 10.1038/s41467-024-55752-3, Wiki.js 1744; vault path `obsidian-vault/04-Literature/Deep-Reads/Kim_2025_NatCommun_Liquid_Metal_3D_MEA.md`, 13,567 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **3 distinct next-gen HD-MEA approaches now in Robin's deep-read set**: (a) **Liu 2026 Nat Biomed Eng** (Rogers Lab flexible-electronics shape-conformal framework, 3000-8000 channels per organoid, >90% surface coverage) — see existing `liu-2026-hd-mea-roadmap-implication` commitment; (b) **Kim 2025 Nat Commun** (EGaIn liquid-metal 3D pillar MEA, 25-310 μm pillar height, 14 μm diameter, parylene-C selective encapsulation, Ni magnetic layer for shape reconfiguration); (c) **3Brain Accura-3D** (rigid CMOS, 4096 electrodes, planar). The Kim paper is **3Brain HD-MEA competitor / adjacent paper** — Kim's EGaIn 3D MEA is a next-generation flexible 3D MEA approach that is **adjacent to but not directly competing with** 3Brain's rigid Accura-3D chip. Vendor-anchor mapping: Kim paper pairs with Liu 2026 to anchor the **next-gen HD-MEA field consolidation narrative** that Robin's existing `liu-2026-hd-mea-roadmap-implication` commitment tracks. Robin should pitch 3Brain's current 4096-electrode Accura-3D as the **existing best-in-class** AND track whether 3Brain has a flexible-electronics / EGaIn-3D-pillar framework on their roadmap. Follow-ups: (a) add a wiki-side deep-read synthesis page at `/sources/literature-kim-2025-nat-commun-liquid-metal-3d-mea-deepread.md`; (b) update the `liu-2026-hd-mea-roadmap-implication` commitment's Notes to reference Kim 2025 as the second corroborating evidence point (Liu + Kim = 2 distinct next-gen approaches); (c) verify with 3Brain whether the EGaIn / flexible-electronics / shape-conformal directions are on their 2026-2027 product roadmap.
+
+### ajongbolo-2025-brain-organoid-review-cluster: pair with Organoids 2026 review to anchor brain-organoid review lane
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `ad4abcde` (Ajongbolo AO, Langhans SA 2025 Cells brain organoid + assembloid review, DOI 10.3390/cells14110842, Wiki.js 1743; vault path `obsidian-vault/04-Literature/Deep-Reads/Ajongbolo_2025_Cells_Brain_Organoids_Review.md`, 11,367 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **Canonical brain organoid + assembloid review** for Robin's deep-read set — the paper establishes a **3-tier taxonomy** (whole-brain → region-specific → complex systems) + **4-class characterization methodology** (morphological + biochemical + functional + omics) + disease-modeling boundaries (neurodevelopmental / neurodegenerative / brain-tumor) + drug-discovery HTS pipeline. **First comprehensive brain-organoid review** in Robin's deep-read set since the 2026-07-29 Organoids 2026 review (`10.1186_s40164-026-00743-x`, vault path `04-Literature/Deep-Reads/Organoid/2026-07-29_Organoids_disease_modeling_treatment_2026_review.md`). Pairs with that review to anchor the **brain-organoid review-paper lane**. Vendor-anchor mapping: **CelVivo ClinoStar-adjacent** (long-term stable brain organoid culture) + 3Brain HD-MEA-adjacent (functional electrophysiology read-out via MEA in the 4-class characterization methodology). Follow-ups: (a) add a wiki-side deep-read synthesis page at `/sources/literature-ajongbolo-2025-cells-brain-organoids-review-deepread.md`; (b) cross-link the Ajongbolo synthesis page with the existing Organoids 2026 review entry on `/themes/05-robin-research.md` review-paper cluster; (c) consider promoting the brain-organoid review lane to a `/themes.md` row if a 2026-08-25 fetch surfaces another brain-organoid paper.
+
+### tian-2025-epicardium-mtor-cardiac-expansion: track cardiac epicardium lane for potential CDMO/regenerative-medicine opportunities
+- Owner: me
+- Source: git-repo-1 2026-08-24 — vault commit `40b357b8` (Tian Y, Lucena-Cacace A, Tani K et al. 2025 Nat Commun hiPSC epicardium mTOR maturation, DOI 10.1038/s41467-025-60934-8, Wiki.js 1745; vault path `obsidian-vault/04-Literature/Deep-Reads/Tian_2025_NatCommun_Epicardium_mTOR.md`, 15,578 bytes, single-shot LDR entry)
+- Due: unscheduled
+- Status: open
+- Notes: **First cardiac-epicardium paper in Robin's deep-read set** — Tian paper establishes **Torin1 (dual mTORC1/2 inhibitor, 200 nM) drives hiPSC epicardium maturation** with mature markers (TJP1/WT1/TBX18/ALDH1A2/CDH18) up + EMT markers down + proliferation markers down + fatty-acid metabolism maturation; 379 DEGs; adult-like profile vs GSE84085; YBX3 + MAFF identified as Torin1-specific TFs; **paracrine TNNI3 upregulation in hiPSC ventricular cardiomyocyte EBs co-cultured for 10 days** (epicardium-to-cardiomyocyte maturation cross-talk). **Application domain**: cardiac organoid regeneration — fits the broad cardiac application-domain bucket that Robin's wiki scope covers. **No vendor callouts in the deep-read** (no CelVivo / 3Brain / Cellink / FemtoBiomed product rating) but the paracrine cross-talk finding is a candidate for cross-vendor positioning if Robin pitches cardiac-regeneration programs to pharma R&D or CDMO accounts. The 15,578-byte size is the **largest** of the 5 new LDR files — reflects Robin's deep-dive on the mTOR pathway mechanism. Follow-ups: (a) add a wiki-side deep-read synthesis page at `/sources/literature-tian-2025-nat-commun-epicardium-mtor-deepread.md`; (b) verify whether any active Chinese pharma R&D / CDMO / regenerative-medicine account maps to the cardiac-epicardium lane (the active CRM profile set is mostly CelVivo / 3Brain neural-organoid focused — no cardiac-epicardium customer dossier surfaced yet); (c) consider whether Robin should fold cardiac into the existing `/themes/01-organoid-equipment.md` theme or open a new `theme-cardiac-regeneration.md` if cardiac papers recur.
+
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